Studies towards an amperometric phosphate ion biosensor for urine and water analysis

Gilbert, L.; Browning, Simon; Jenkins, A. Toby A.; Hart, John P.

doi:10.1007/s00604-010-0316-2

Cited by 16 publications

(16 citation statements)

References 27 publications

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“…Detection was conducted at +0.8 V . Pyruvate oxidase immobilized cobalt phthalocyanine screen‐printed carbon electrode based biosensor was operated at +0.3 V and +0.4 V . In another report, pyruvate oxidase was added into working buffer to detect phosphate, and H 2 O 2 electrode was used to monitor H 2 O 2 at +0.7 V .…”

Section: Resultsmentioning

confidence: 99%

Poly(pyrrole‐co‐pyrrole‐2‐carboxylic acid)/Pyruvate Oxidase Based Biosensor for Phosphate: Determination of the Potential, and Application in Streams

2019

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A biosensor based on conductive poly(pyrrole‐co‐pyrrole‐2‐carboxylic acid) [Poly(Py‐co‐PyCOOH)] copolymer film coated gold electrode was developed for the quantitative phosphate determination. Enzyme pyruvate oxidase was immobilized chemically via the functional carboxylated groups of the copolymer. The potential to be applied which is deficiency of phosphate biosensor studies for precise phosphate detection was clarified by using differential pulse voltammetry technique. Performance of the sensing ability of the biosensor was improved by optimizing cofactor/cosubstrate concentrations, polymeric film density and pH. The biosensor showed a linearity up to phosphate concentration of 5 mM, operational stability with a relative standard deviation (RSD) of 0.07 % (n=7) and accuracy of 101 % at −0.15 V (vs. Ag/AgCl). Detection limit (LOD) and sensitivity were calculated to be 13.3 μM and 5.4 μA mM−1 cm−2, respectively by preserving 50 % of its initial response at the end of 30 days. It's performance was tested to determine phosphate concentrations in two streams of Zonguldak City in Turkey. Accuracy of phosphate measurement in stream water was found to be 91 %.

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Section: Resultsmentioning

confidence: 99%

Poly(pyrrole‐co‐pyrrole‐2‐carboxylic acid)/Pyruvate Oxidase Based Biosensor for Phosphate: Determination of the Potential, and Application in Streams

2019

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“…The upper limit of linearity was found to be 5 mM, while the limit of detection (LOD) was determined as 50 mM, equivalent to three times the blank value. Thus, obtained LOD is much lower than the concentration of phosphate found in human blood serum, and also comparatively better than the detection limit of some recently reported amperometric phosphate biosensors (Gilbert et al, 2010;Lawal and Adeloju, 2013).…”

Section: Inhibition Curvementioning

confidence: 95%

Alkaline phosphatase inhibition based conductometric biosensor for phosphate estimation in biological fluids

Upadhyay

Verma

2015

Biosensors and Bioelectronics

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“…Furthermore, cobalt-based materials continue to attract considerable interest, mainly due to their excellent electrocatalytic activity toward various compounds, such as glucose, L-glutathione, hydrogen peroxide, phosphate and methanol [18][19][20][21][22]. Recently, Adekunle et al [23] made a composite electrode integrated with cobalt nanoparticles (Co NPs) and single-walled carbon nanotubes on the edge plane pyrolytic graphite electrode, which showed excellent catalytic characteristics for the electrochemical oxidation of nitrite.…”

Section: Introductionmentioning

confidence: 99%

A nanomaterial composed of cobalt nanoparticles, poly(3,4-ethylenedioxythiophene) and graphene with high electrocatalytic activity for nitrite oxidation

Wang

2012

Microchim Acta

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We have investigated the oxidative electrochemistry of nitrite on glassy carbon electrodes modified with cobalt nanoparticles, poly(3,4-ethylenedioxythiophene) (PEDOT), and graphene. The modified electrode was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, and scanning electron microscopy. The results suggest that this new type of electrode combines the advantages of PEDOT-graphene films and cobalt nanoparticles and exhibits excellent electrocatalytic activity towards the oxidation of nitrite. There is a linear relationship between the peak current and the nitrite concentration in the range from 0.5 μM to 240 μM, and the detection limit is 0.15 μM. The modified electrodes also enable the determination of nitrite at low potentials where the noise level and interferences by other electro-oxidizable compounds are weak.

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Studies towards an amperometric phosphate ion biosensor for urine and water analysis

Cited by 16 publications

References 27 publications

Poly(pyrrole‐co‐pyrrole‐2‐carboxylic acid)/Pyruvate Oxidase Based Biosensor for Phosphate: Determination of the Potential, and Application in Streams

Poly(pyrrole‐co‐pyrrole‐2‐carboxylic acid)/Pyruvate Oxidase Based Biosensor for Phosphate: Determination of the Potential, and Application in Streams

Alkaline phosphatase inhibition based conductometric biosensor for phosphate estimation in biological fluids

A nanomaterial composed of cobalt nanoparticles, poly(3,4-ethylenedioxythiophene) and graphene with high electrocatalytic activity for nitrite oxidation

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