The objective of the present study was to investigate the relationship between the indices of body size such as BMI, fat-free mass index (FFMI, FFM/height 2 ), fat mass index (FMI, FM/height 2 ), and body fat percentage (%BF), and physical activities assessed by the doublylabelled water (DLW) method and an accelerometer in free-living Japanese adult women. We conducted a cross-sectional study in 100 female subjects ranging in age from 31 to 69 years. Subjects were classified in quartiles of BMI, FFMI, FMI and %BF. Daily walking steps and the duration of light to vigorous physical activity were simultaneously assessed by an accelerometer for the same period as the DLW experiment. Only physical activity-related energy expenditure (PAEE)/FFM and PAEE/body weight (BW) decreased in the highest quartile of BMI. Physical activity level, PAEE/FFM and PAEE/BW decreased in the highest quartile of FMI and %BF, whereas they were not different among quartiles of FFMI. Daily walking steps and the duration of moderate-and vigorous-intensity physical activities decreased or tended to decrease in the highest quartile of FMI and %BF, but did not differ among quartiles of FFMI and BMI. These results clearly showed that Japanese adult women with higher fat deposition obviously had a low level of physical activities assessed by both the DLW method and accelerometry, but those with larger BMI had lower PAEE/FFM and PAEE/BW only. Our data suggest that the relationship between obesity and daily physical activities should be discussed using not only BMI but also FMI or %BF.Key words: Body composition: Physical activity: Doubly-labelled water: Accelerometry: Japanese adult women Obesity is caused by an imbalance between energy intake and energy expenditure. Obese individuals are often considered to be physically less active than normal-weight individuals. However, most cross-sectional studies using the doubly-labelled water (DLW) method, which is known to be the most accurate method of measuring energy expenditure in free-living conditions (1,2) , have reported that physical activity level (PAL; the ratio of total energy expenditure(TEE):BMR) did not differ among BMI categories (3 -6) . The reason for the lack of this association may be partly explained by differences in the distribution of fat-free mass (FFM) and fat mass (FM). PAL appears to be negatively associated with FM (7,8) , but not correlated with FFM (5) . However, these studies have only reported information on the association between PAL and either FM or FFM, which are not adjusted for body size, such as body height. To our knowledge, no information is available from thoroughly examining the relationship between BMI or body composition, i.e. FFM index (FFMI, FFM divided by height squared), FM index (FMI, FM divided by height squared) or body fat percentage (%BF) and physical activity in adult women, particularly in Asian populations.Recently, many cross-sectional studies on adult women in Western countries and Japan reported that BMI and %BF were inversely associate...