The enhanced body activities after taking the nutritional addendum by athletes were observed via fluctuation in the clinical biomarkers. There are some previous evidences recorded that the nutritional supplements have a benefit and side influences, hence current paper was aimed to determine the potential influences of nutritional support compounds on biochemical parameters and kidneys damage among bodybuilders people. Two subjects of sportsmen bodybuilders; first group not take the nutritional supplements (control group), whereas the second group take the nutritional supplements (users group) were included within current study and screened for their serological level of creatinine, urea, total protein and albumin with measuring urine total protein and pH. The results showed that the mean titer of serum creatinine, serum albumin and urine total protein were significantly higher among users group compared to control group, whereas the level of serum urea, serum total protein and urine pH revealed non-significant different between both groups. For subjects aware to the risks and side effects of taking the nutritional supplements, the users group exhibited a significant higher frequency % of unaware to the risks in comparison to control group. In denouement, there is prodromal defect in the kidneys of the nutritional supplements consumers, confirmed by serum creatinine, serum albumin and urine total protein increase. Many of nutritional supplements users not have enough information about the consequences and side effects of the decompounds hence exercising related with abuse of it without consulting a dietitian may be hazardous.