The powder protein is mostly used as the main dietary by bodybuilders for improving exercise training. This research studied the effect of the powder protein intake on serum total protein and creatinine concentrations, and urine pH and protein of three different groups. The serum total protein concentration has no relationship with the amount of powder protein intake and cannot be affected by it. But the serum creatinine concentration has a direct relationship with the amount of powder protein intake. The group A and B serum creatinine concentrations in their blood were abnormal due to lack in the amount of protein intake in their daily life and exercise. The group C used sufficient amounts of powder protein 1.2-1.4 gm/Kg/day, which maintained their creatinine concentration. This quantity of protein intake for bodybuilders is not only safe, but also is improved the training adaptations in their excises training. The urine tests indicated no significant differences (P < 0.01) between their pH and at the same time the trace amount of protein in some of individuals urine was observed.