Study of 5-Fluorouracil Loaded Chitosan Nanoparticles for Treatment of Skin Cancer

Abstract

Background: The purpose of this study was to formulate, characterize and in-vitro cytotoxicity of 5-Fluorouracil loaded controlled release nanoparticles for the treatment of skin cancer. The patents on nanoparticles (US8414926B1), (US61654404A), (WO2007150075A3) etc helped in the selection polymers and method for the preparation of nanoparticles. Methods: In the present study nanoparticles were prepared by simple ionic gelation method using various concentrations of chitosan and sodium tripolyphosphate. Several process and formulation parameters were screened and optimized using 2^5-2 fractional factorial design.The prepared nanoparticles were evaluated for particle size, shape, charge, entrapment efficiency, crosslinking mechanism and drug release study Results: The optimized 5-Fluorouracil loaded nanoparticle were found with particle size of of 320±2.1 nm, entrapment efficiency of 85.12%± 1.1% and Zeta potential of 29mv±1mv. Scanning electron microscopy and dynamic light scattering technique revealed spherical particles with uniform size. The in-vitro release profile showed controlled release up to 24 hr. Further study was carried using A375 basal cell carcinoma cell-line to elucidate the mechanism of its cytotoxicity by MTT assay. These results demonstrate that the possibility of delivering 5-Fluorouracil to skin with enhanced encapsulation efficiency indicating effectiveness of the formulation for treatment of basal cell carcinoma type of skin cancer.

Amelioration of Cancer Employing Chitosan, Its Derivatives, and Chitosan-Based Nanoparticles: Recent Updates

“…Chitosan ---------5-Fluorouracil Skin cancer Provided improved inhibitory effect on cancer cell lines than drug solutions. [115] Folic acid-coated CSNPs MW = 310,000-375,000, DD = 83% 5-Fluorouracil Colon cancer Provided improved cytotoxicity of the NPs on coating with folic acid than uncoated CSNPs. [27] Chitosan -------------Chlorambucil Breast cancer…”

Dacarbazine-encapsulated solid lipid nanoparticles for skin cancer: physical characterization, stability, in-vivo activity, histopathology, and immunohistochemistry

“…As per Patel Gayatri et al (2020), by ionic gelation method and in the presence of chitosan and sodium tripolyphosphate (TPP), 5-Fluorouracil loaded nanoparticles were prepared. The cytotoxicity studies were carried out by MTT assay using A375 basal cell carcinoma cell-line (15). For topical drug delivery, the solid lipid nanoparticles approach would be the best possible solution to treat metastatic malignant melanoma (16,17).The prepared solid lipid nanoparticles can be stabilized by using suitable surfactant and co-surfactants.…”

Synthesis and performance evaluation of 5-fluorouracil-loaded zwitterionic poly(4-vinylpyridine) nanoparticles

“…32,33 Several studies in the field of 5-FU-loaded polymer NPs can be found in the literature. 28,[34][35][36] For example, Rahmani and coworkers 37 prepared silk fibroin NPs containing 5-FU as a reliable drug delivery system for cancer treatment with high loading capacity and sustained release profile.…”

“…32,33 Several studies in the field of 5-FU-loaded polymer NPs can be found in the literature. 28,34–36 For example, Rahmani and coworkers 37 prepared silk fibroin NPs containing 5-FU as a reliable drug delivery system for cancer treatment with high loading capacity and sustained release profile. Their results displayed that by increasing the amount of drug, a deformation occurred in the structure of the NPs, which reduced the particle size, increased the loading efficiency and revealed slower and more controlled release.…”