Study of antibacterial and antifungal efficacy of platelet-rich fibrin and platelet-rich fibrin matrix

Abstract

Background: Platelet concentrates are extensively utilized in the medical and dental field to promote tissue regeneration. The profusion of endogenous growth factors in platelets α-granules transmit their use for enhanced wound healing. However, little attention has been given to study their antimicrobial potential. This study was conducted to assess the antibacterial and antifungal property of platelet-rich fibrin (PRF) and PRF matrix (PRFM). Materials and Methodology: Blood samples were obtained from 16 participants, PRF and PRFM were processed as per the protocol prescribed by Choukroun et al . and Lucarelli et al ., respectively. The susceptibility test against microbiota in the root canal and Candida albicans was assessed through minimum inhibition zone by agar diffusion technique. Results: PRF showed an effective antibacterial property, however, did not perform well against C. albicans strains. PRFM did not show any antibacterial or antifungal properties. Conclusions: The antibacterial efficacy of PRF may prove beneficial when used in the revascularization procedure of immature necrotic teeth.

Preliminary Novel Analysis on Antimicrobial Properties of Concentrated Growth Factor against Bacteria-Induced Oral Diseases

“…Existing literatures showed that the other platelet concentrate forms such as the PRF and i-PRF showed antibacterial effects against a wide array of microorganisms including the Candida albicans, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans by showing positive zone of inhibition with no available reports on the CGF action as an antibacterial agent. 21,38,39 Despite the established data on the antibacterial effect, the exact component within the platelet concentrate and mechanism involved for its antibacterial effect are still largely unknown, and the anecdotal and empirical evidence claiming the production of oxygen ion and metabolites, various antibacterial action including aggregation and binding to the microorganisms, the release of host defense antimicrobial peptides, direct interaction of platelets with microorganisms, specific activation of antioxidants, and production of particular proteins such as defensins, lactoferrins, and cathelicidins that are involved in the host-specific immune response were suggested. 21,[39][40][41][42] Bacterial biofilms are serious global health issues and have emerged as key factors in antibiotic resistance due to their abilities to tolerate antibiotics, host defense systems, and other external stresses.…”

Can Our Blood Help Ensure Antimicrobial and Anti-Inflammatory Properties in Oral and Maxillofacial Surgery?

“…Clinical isolates are usually obtained from bacterial samples taken from the oral cavity, such as biofilm-producing Staphylococcus species S. aureus and S. epidermidis [ 68 ]. However, some studies do not specify microorganisms but use the whole spectrum of supragingival plaque and dental root canal microflora specimens without clarification [ 60 , 69 , 70 , 71 ].…”

Otolog Trombosit Konsantrelerinin İmmunolojik ve Antimikrobiyal Etkileri

“…PRF is the secondary origination platelet concentration and contains biologically active proteins that promote curative healing and tissue repair but induce a moderate anti-inflammatory immune response. PRF is a true biomaterial that contains fibrin clots, platelets, leukocytes, and growth factors and it can clear pathogens from the bloodstream via binding and removing microorganisms (Kazemi et al 2014;Nagaraja et al 2019). PRF has recently been used to promote healing, bone recovery, graft stabilization, biofilm inhibition, and catheter hygiene.…”

“…Αgranules accommodate large adhesive and curative proteins and are also host to many different types of big proteins, such as coagulation factors and protease inhibitors. They also take part in antibody-mediated cytotoxicity tasks to eliminate pathogens, and at the end of the immunological process, platelets secrete a kind of potent antimicrobial peptide (Kazemi et al 2014;Nagaraja et al 2019). The inflammatory stage may be accelerated by coagulation and platelet degranulation.…”