Background: Platelet concentrates are extensively utilized in the medical and dental field to promote tissue regeneration. The profusion of endogenous growth factors in platelets α-granules transmit their use for enhanced wound healing. However, little attention has been given to study their antimicrobial potential. This study was conducted to assess the antibacterial and antifungal property of platelet-rich fibrin (PRF) and PRF matrix (PRFM). Materials and Methodology: Blood samples were obtained from 16 participants, PRF and PRFM were processed as per the protocol prescribed by Choukroun et al . and Lucarelli et al ., respectively. The susceptibility test against microbiota in the root canal and Candida albicans was assessed through minimum inhibition zone by agar diffusion technique. Results: PRF showed an effective antibacterial property, however, did not perform well against C. albicans strains. PRFM did not show any antibacterial or antifungal properties. Conclusions: The antibacterial efficacy of PRF may prove beneficial when used in the revascularization procedure of immature necrotic teeth.

Management of diabetes mellitus is a challenge for clinicians. Uncontrolled hyperglycemia increases the risk of microvascular and macrovascular complications, damaging the body systems.  Although a number of antidiabetic drugs are available for therapeutic intervention, toxicity, loss of efficacy in chronic use and high cost of treatment have necessitated the search for new molecules to manage diabetes. Safety and cost are the main prerequisite for the new antidiabetic molecules. Medicinal plants and their purified phytochemicals have shown promising antidiabetic potential in the past few years. The flavonoids can be widely classified into different categories like anthocyanins, catechins, flavanols, flavones, flavanones etc. Some flavonoids have hypoglycemic properties. They may improve al-tered glucose and oxidative metabolisms of diabetic states. The hypoglycemic effect of some herbal extracts has been confirmed in human and animal models of type 2 diabetes mellitus (T2DM). Some of the important phytoconstituents from the classes of flavonoid have been discussed here. The current review summarizes the  antidiabetic activity of flavonoids, the mechanism-based action of flavonoids that target the various metabolic pathways in humans. Keywords: Diabetes mellitus, Flavonoids, Medicinal plants, mechanisms of action, T2DM

Background: Neuraminidase enzymes are a large family found in a range of organisms. The best-known neuraminidase is viral neuraminidase, a drug target for the prevention of the spread of influenza infection. The viral neuraminidases are frequently used as antigenic determinants found on the surface of the influenza virus. background: Studied on a 28 series compound and came with the most potent drug towards Influenza Objective: Thiazolidine derivatives have been synthesized and explored previously, and further compounds have been designed on the basis of leading compounds. This research aimed to validate those previously synthesized compounds and a new series of compounds. objective: To make the most potent drug with enhanced biological activity. Methodology: A series of 28 compounds of thiazolidine-4-carboxylic acid derivatives were studied and evaluated for their ability to inhibit the neuraminidase (NA) of the influenza A virus. Twenty-eight compounds were differentiated into a training set of 21 compounds and a test set of 07 compounds. method: A series of 28 compounds of thiazolidine-4-carboxylic acid derivatives was studied and evaluated for their ability to inhibit neuraminidase (NA) of influenza A virus. Compound was drawn on ChemSketch and further evaluated. Results: The validated compounds demonstrated moderate inhibitory activity against influenza A neuraminidase. The most potent compound was acetaminophen mercapturate (C13H16N2O5S) (MW: 312.34). S-(5-acetamido-2-hydroxyphenyl)-N-acetyl-L-cysteine is an S-substituted N-acetyl-L-cysteine in which the S-substituent is specified as 5-acetamido-2-hydroxyphenyl. It acts as a drug metabolite, a human urinary metabolite, and a rat metabolite. It is a member of acetamides, an organic sulphide, a member of phenols and an S-substituted N-acetyl-L-cysteine. It derives from “paracetamol”. result: The most potent compound is Acetaminophen mercapturate (C13H16N2O5S) (MW: 312.34) S-(5-acetamido-2-hydroxyphenyl)-N-acetyl-L-cysteine is an S-substituted N-acetyl-L-cysteine in which the S-substituent is specified as 5-acetamido-2-hydroxyphenyl. Conclusion: Validation of inhibitory activity of thiazolidine-4-carboxylic acid derivatives as novel influenza NA shows drug discovery of a more potent and reliable drug for the influenza virus. conclusion: All the studies and evaluation carried out in this paper is only to get the most potent compound or drug with enhanced biological activity. other: As to it, 10 predicted compounds also being proposed in the given paper

In the present study the methanol extract of a novel polyherbal formulation (PHF) was studied for alpha (α)- amylase and alpha (α)-glucosidase inhibition using an in vitro antidiabetic model. The polyherbal extract were also examined for its antioxidant activity by using free radical 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging method. The study revealed that the polyherbal formulation exhibit potent radical scavenging activity using DPPH as substrate. The methanol extract exhibited significant α-amylase and α-glucosidase inhibitory activities with an IC50 value 31.52±0.74µg and 53.13±0.97µg respectively and well compared with standard acarbose drug. Further, antibacterial activity of methanol extract of PHF valuated against standard strains, the tested extract showed more potent inhibitory effects on both Gram (+) bacteria and  Gram (-) bacteria. Thus, it could be concluded that due the presence of antioxidant components the plant extract have well prospective for the management of diabetes and the related condition of oxidative stress. This knowledge will be useful in finding more potent antidiabetic principle from the natural resources for the clinical development of antidiabetic therapeutics.

No abstract