Study of Antibacterial Efficacy of Hybrid Chitosan-Silver Nanoparticles for Prevention of Specific Biofilm and Water Purification

Abstract

Antibacterial efficacy of silver nanoparticles (Ag NPs) deposited alternatively layer by layer (LBL) on chitosan polymer in the form of a thin film over a quartz plate and stainless steel strip has been studied. An eight-bilayer chitosan/silver (Cs/Ag)8 hybrid was prepared having a known concentration of silver. Techniques such as UV-visible spectroscopy, inductively coupled plasma optical emission spectroscopy (ICP-OES), and atomic force microscopy (AFM) were carried out to understand and elucidate the physical nature of the film. Gram-negative bacteria, Escherichia coli (E. coli), were used as a test sample in saline solution for antibacterial studies. The growth inhibition at different intervals of contact time and, more importantly, the antibacterial properties of the hybrid film on repeated cycling in saline solution have been demonstrated. AFM studies are carried out for the first time on the microbe to know the morphological changes affected by the hybrid film. The hybrid films on aging (3 months) are found to be as bioactive as before. Cytotoxicity experiments indicated good biocompatibility. The hybrid can be a promising bioactive material for the prevention of biofilms specific to E. coli and in purification of water for safe drinking.

Applications of chitosan powder with in situ synthesized nano ZnO particles as an antimicrobial agent

“…4 the three composites show similar absorbance and their value of λ max is 5 almost close to each other supporting the XRD results. 16 The band gap energy of chitosan-nanoZnO composites powder was measured from 17 absorption spectra using the following equation:…”

“…All the chemicals were of analytical grade reagents and used 14 without further purification. 15 16 Chitosan solution was prepared by dissolving 0.5 g of chitosan in 2 % acetic acid 17 solution in room temperature. The solution was then filtered to remove undissolved particles 18 and added 30 mL of 15 % zinc acetate dihydrate solution and shaken well.…”

“…The FTIR spectra of the samples were recorded in KBr pellets using Fourier 16 The antimicrobial activity of chitosan and chitosan-based nanocomposite powders 17 were evaluated by Agar well diffusion method. Gram-negative bacteria E.coli (MTCC 1687) and S.aureus) was evaluated by disc diffusion method.…”

A Review on Antimicrobial Chitosan-Silver Nanocomposites: A Roadmap Toward Pathogen Targeted Synthesis

“…A chitosan=silver (CS=Ag) hybrid thin coating was deposited over a quartz and stainless steel strip via a layer-bylayer process [50]. AgNPs of a size of 50 nm were prepared by chemical reduction of silver nitrate, capped with sodium citrate, and aqueous acetic acid solution of chitosan was utilized for chitosan coatings.…”

Preparation, Structure, and Properties of Hybrid Polymer Composites Containing Silver Clusters and Nanoparticles

“…The OH and CO 2 H groups of polysaccharides may participate both in the reduction of Ag + ions and stabilization of SN. Upon the deposition of chitosan and SN acting as physical cross-linking sites, whose presence enhances the elasticity and decreases the hydrophilicity of these systems [146], biocompatible multilayer coatings with enhanced mechanical strength and marked bactericidal activity for specific SN concentration <2·10 -5 g/cm 2 are obtained on an inert base [147]. Composites with enhanced mechanical properties and thermal stability may be prepared using a mixed polymer matrix derived from the polysaccharide, pullulan, along with PVA and SN [148].…”