Study of Antiobesity Effect through Inhibition of Pancreatic Lipase Activity ofDiospyros kakiFruit andCitrus unshiuPeel

Abstract

Pancreatic lipase is the enzyme responsible for digestion and absorption of triglycerides, being its inhibition one of the widest studied methods used to determine the potential activity of natural products to inhibit dietary fat absorption. Decrease of energy intake from dietary fat through inhibition of this enzyme may be an excellent strategy to prevent and treat obesity. The inhibitory activity on pancreatic lipase enzyme of Diospyros kaki fruit and Citrus unshiu peel mixture extract (PCM) was evaluated in vitro and its antiobesity effects were studied based on the serum lipid parameters analysis from high-fat diet- (HFD-) fed mice in vivo. PCM was orally administered at a dose of 50 and 200 mg/kg body weight for 6 weeks. In addition, the activity of pancreatic lipase was assessed using orlistat (positive control). PCM exhibited inhibitory effect on lipase activity with IC50 value of 507.01 μg/mL. Moreover, serum triacylglycerol, total cholesterol levels, and visceral fat weight were significantly reduced compared to HFD control mice in PCM 200 mg/kg-treated mice (p < 0.05). These results suggest that PCM administration may be a novel potential antiobesity agent for reduction of fat absorption via inhibition of pancreatic lipase.

In vitro effects of four polysaccharides containing β-D-Glu p on intestinal function

“…Indeed, decrease energy intake from dietary fat through inhibition of this enzyme is an essential strategy adopted to prevent and treat obesity. [38] In this study, pancreatic lipase activity was analyzed after polysaccharides addition, and all polysaccharides significantly (p < .05) decreased pancreatic lipase activity, with inhibitory effects that were proportional to the concentration of polysaccharides (p < .05) ( Table 6). As we mentioned above, calcium reacts with liberated free fatty acids using ionic complexation, thereby removing them from the surface of the lipid droplets and preventing them from inhibiting the lipase activity, and therefore calcium concentration has a negative dose-dependent inhibitory effect on pancreatic lipase.…”

Inhibitory Activities of Camellia Mistletoe (K. Japonica) Extracts on Pancreatic Lipase, Tyrosinase and Cancer Cell Proliferation

“…Nevertheless, this was weak (IC50 value of 0.39 mg/ml), compared to that for the positive control of hesperidin (IC50 value of 0.032 mg/ml). However, the pancreatic lipase inhibitory effect of Camellia mistletoe was superior to those when various dietary plants were screened by Kim et al [17] and Conforti et al [18].…”

Citrus × Clementina Hort. Juice Enriched with Its By-Products (Peels and Leaves): Chemical Composition, In Vitro Bioactivity, and Impact of Processing

“…Among Citrus phytochemicals, flavonoids are mainly involved in the management of T2DM. These compounds are able to (i) inhibit carbohydrate-hydrolysing enzymes [17]; (ii) inhibit sodium-dependent glucose transporter 1 (SGLT1) [67] (iii) stimulate insulin secretion; (iv) reduce hepatic glucose output; and (v) enhance insulin-dependent glucose uptake [68]. In particular, the main abundant flavonoid of C. × clementina, neohesperidin, inhibited both α-amylase and α-glucosidase in a concentration-dependent manner and was more active than acarbose.…”