Study of antioxidant capacity and metabolization of quebracho and chestnut tannins through in vitro gastrointestinal digestion-fermentation

Molino, Silvia; Fernández‐Miyakawa, Mariano E.; Giovando, Samuele; Rufián-Henares, José Ángel

doi:10.1016/j.jff.2018.07.056

Cited by 53 publications

(47 citation statements)

References 37 publications

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“…On the other hand, in vitro digested Ch and Qu50%-Ch50% showed a higher antioxidant activity compared to Qu. Our data are in line with those of Molino et al [31] who reported a higher antioxidant activity for Ch (8.16 mmol Trolox/g) compared with Qu (6.70 mmol Trolox/g) after in vitro digestion. In general, compared to our results, they reported higher values of antioxidant capacity in their samples, which may be due to the different methodology used for antioxidant evaluation (GAR method) and the different in vitro digestion protocol used in their study.…”

Section: Discussionsupporting

confidence: 93%

In Vitro Digestion of Chestnut and Quebracho Tannin Extracts: Antimicrobial Effect, Antioxidant Capacity and Cytomodulatory Activity in Swine Intestinal IPEC-J2 Cells

Reggi

Giromini

Dell’Anno

et al. 2020

Animals

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Quebracho (Qu) and chestnut (Ch) are natural sources of tannins and they are currently used in animal nutrition as feed ingredients. However, to date the bio-accessibility, antimicrobial, antioxidant, and intestinal epithelial cell stimulatory doses of Qu and Ch have not been determined. Our study investigates the antioxidant and E. coli F4+ and F18+ growth inhibitory activity of Qu, Ch, and their combinations after solubilization in water (to evaluate the already bio-accessible molecules) and after simulated gastro-intestinal digestion in vitro. The effect of an in vitro digested Ch and Qu combination was also tested on intestinal epithelial IPEC-J2 cells experimentally stressed with hydrogen peroxide (H2O2) and Dextran Sodium Sulfate (DSS). The results showed that undigested Qu and Ch alone, and in combination, exerted a valuable antioxidant capacity and E. coli F4+ and F18+ growth inhibitory activity. The concentration of 1200 µg/mL exhibited the highest E. coli growth inhibitory activity for all the samples tested. In addition, after in vitro digestion, Qu and Qu50%–Ch50% maintained E. coli growth inhibitory activity and a modest antioxidant capacity. Three hours pre-treatment with in vitro digested Qu50%–Ch50% counteracted the H2O2 and DSS experimentally-induced stress in the intestinal IPEC-J2 cells. Ch and Qu tannin extracts, particularly when combined, may exert E. coli F4+ and F18+ growth inhibitory activity and valuable antioxidant and cell viability modulation activities.

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Section: Discussionsupporting

confidence: 93%

In Vitro Digestion of Chestnut and Quebracho Tannin Extracts: Antimicrobial Effect, Antioxidant Capacity and Cytomodulatory Activity in Swine Intestinal IPEC-J2 Cells

Reggi

Giromini

Dell’Anno

et al. 2020

Animals

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“…We hypothesized that CWE as a source of hydrolysable tannins was well absorbed and enhanced digestive enzyme activities in the gastrointestinal tract of experimental broilers that may had a role in improving crude protein digestibility in this study. Molino et al. (2018) observed that chestnut hydrolysable tannins were easily available for microbial fermentation as it has prebiotic activity that may be beneficial for nutrient utilization.…”

Section: Discussionmentioning

confidence: 99%

Effect of chestnut wood extract on performance, meat quality, antioxidant status, immune function, and cholesterol metabolism in broilers

Liu

Mahfuz

et al. 2020

Poultry Science

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Plant extracts have been proved as natural antioxidants resources as well as alternative feed additives in livestock and poultry species. Chestnut wood extract ( CWE ) as a source of hydrolysable tannic acid was used to evaluate the growth performance, nutrient retention, meat quality, antioxidant status, and immune function of broilers. A total of 168, day-old Arbor Acre male broilers (weight 46.59 ± 0.44 g) were randomly divided to 3 treatments, 7 replicate pens per treatment, 8 broilers per pen. The treatments contain a control diet, CON (corn-soybean meal basal diet); an antibiotic diet, CTC (basal diet + 75 mg/kg chlortetracycline); and chestnut wood extract diet, CWE (basal diet + 1,000 mg/kg chestnut tannins). At the finisher phase, final body weight was higher ( P < 0.05) in CWE supplemented diet than in CON. Average daily body weight gain was higher ( P < 0.05) and feed gain ratio was lower ( P < 0.05) in broilers fed CWE than in those fed CON at the finisher phase. Crude protein digestibility was higher ( P < 0.05) in broilers offered CWE than that in broilers fed CON and CTC diets. Breast muscle pH value at 24 h (pH 24 h ) was higher ( P < 0.05) in broilers fed CWE than that in those fed CON and CTC diets. The bursa weight was higher ( P < 0.05) in broilers offered CWE than that in those fed CON and CTC. Total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) values were higher ( P < 0.05) in both breast muscle and thigh muscle of broilers offered CWE supplemented diet than those in broilers fed CON and CTC diets. Similarly, broilers offered with CWE diets showed higher ( P < 0.05) T-AOC, GSH-PX, and SOD value in serum than those fed CON and CTC diets. Serum concentration of IgG was higher ( P < 0.05) in broilers offered with CWE diets than that in those fed CON and CTC diets. Total cholesterol, low-density lipoprotein cholesterol, and urea-N concentration were lower ( P < 0.05) in broilers offered CWE diet than those in broilers fed CON and CTC diets. It was recommended to supply CWE at the 1,000 mg/kg level for improving antioxidant status, cholesterol metabolism, and growth performance without affecting normal meat quality in broilers.

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“…An interesting note by Diaz Carrasco et al [2] was the sensitivity effect that dietary tannins had on Gram-positive bacteria, as also noted in rat studies with increased Gram-negative bacteria in the gastrointestinal tract [46]. Molino et al [47] have shown preliminary data on the importance of tannins in gut microbial fermentation and the nutritional importance in human application. This study provides additional new information on affected pathways in the intestine with the introduction of ChTs in early broiler growth.…”

Section: Discussionmentioning

confidence: 93%

Chestnut tannins and their relationship in promoting broiler growth by altering the metabolic phenotype in the cecum.

Lee

Pont

Jarvis³

et al. 2020

Preprint

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Background As the demand for alternatives to antibiotic growth promoters (AGPs) increases in food animal production, phytobiotic compounds gain in popularity due to their ability to mimic the desirable bioactive properties of AGPs. Chestnut tannins (ChT) are one of many phytobiotic compounds utilized as feed additives, particularly in South America, for broiler chickens due to its favorable antimicrobial and growth promotion capabilities. While studies have observed the microbiological and immunological effects of ChT, there is a lack of studies evaluating the metabolic function of ChT in the host. Therefore, the objective of this study was to characterize the cecal metabolic changes induced by ChT inclusion and how they relate to growth promotion. Results A total of 500 day-of-hatch Cobb 500 chicks were separated into five feed treatment groups: control, 1% ChT, 0.2% ChT, 0.08% ChT, and 0.03% ChT. The cecas from all the chicks in the treatment groups were collected on days 2, 4, 6, 8, and 10 post-hatch. The cytokine mRNA qRT-PCR was determined using TaqMan gene expression assays for IL-1B, IL-6, IL-8, IL-10, and IFN-γ quantification. The cytokine expression showed highly significant increased expressions of IL-6 and IL-10 on days 2 and 6, while the other pro-inflammatory cytokines did not have significantly increased expression. The results from the kinome array demonstrated that the cecas from birds fed with 1% ChT had significant (p < 0.05) metabolic alterations based on the number of peptides when compared to the control group across all days tested. Conclusions Based on the results, the increased expression of IL-6 appeared to be strongly indicative of altered metabolism while the increased expression of IL-10 indicated the regulatory effect against other pro-inflammatory cytokines other than IL-6. The ChTs initiate a metabolic mechanism during the first ten days in the broiler. For the first time, we show that a phytobiotic product initially modulates metabolism rather than immunity while also potentially supporting growth and feed efficiency downstream. In conclusion, an IL6-mediated immunometabolic phenotypic alteration in the ceca of chickens fed ChT may indicate the importance in the gut of enhanced broiler health.

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Study of antioxidant capacity and metabolization of quebracho and chestnut tannins through in vitro gastrointestinal digestion-fermentation

Cited by 53 publications

References 37 publications

In Vitro Digestion of Chestnut and Quebracho Tannin Extracts: Antimicrobial Effect, Antioxidant Capacity and Cytomodulatory Activity in Swine Intestinal IPEC-J2 Cells

In Vitro Digestion of Chestnut and Quebracho Tannin Extracts: Antimicrobial Effect, Antioxidant Capacity and Cytomodulatory Activity in Swine Intestinal IPEC-J2 Cells

Effect of chestnut wood extract on performance, meat quality, antioxidant status, immune function, and cholesterol metabolism in broilers

Chestnut tannins and their relationship in promoting broiler growth by altering the metabolic phenotype in the cecum.

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