Study of Arsenic-Contaminated Soil Bacterial Community Using Biochip Technology

Bunin, Evgeni; Khatisashvili, Gia; Varazi, Tamar; Kartvelishvili, Tamar; Asatiani, Nino; Sapojnikova, Nelly

doi:10.1007/s11270-020-04575-1

Cited by 5 publications

(2 citation statements)

References 34 publications

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“…Even though specific probes for target microorganisms are generated following well-established requirements, this would require an experimental confirmation of their equal hybridization capacity. The experimental cassette approach proves the selected probes equal hybridization capacity [22,26]. Therefore, the variations in the fluorescent intensities on a biochip, coming from probe′s size and nucleotide sequence differences are excluded.…”

Section: Biochip Design: Dna Probes and Biochip Matrixmentioning

confidence: 99%

“…The signals from the probes 1-3 can only confirm or disprove the presence of the target species in the soil samples and can show the comparative relation between the studied samples. Biochip dendrimeric matrix technology and conditions of hybridization on a biochip were the same as described previously [26].…”

Section: Biochip Design: Dna Probes and Biochip Matrixmentioning

confidence: 99%

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Remedial Approaches against Arsenic Pollution

Khatisashvili¹,

Varazi²,

Kurashvili³

et al. 2022

Arsenic Monitoring, Removal and Remediation

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The study is devoted to a very urgent and acute problem for Georgia – remediation/restoration of the arsenic (As) mining and storage sites. The approach of a given work is based on using capabilities of nature itself, which has a great adaptive potential to chemical environmental pollution. The aim of the study is to identify the bacterial strains from the endemic soil microbiota, characteristic to a specific localization of arsenic contaminated sites and able to resist to the toxicant. To determine the level of arsenic contamination, soil samples have been analyzed using Inductively Coupled Plasma - Optical Emission Spectrometry method. The distribution of arsenic in soil samples splits them into categories according to the degree of contamination, ranging from 50 ppm to 13000 ppm. The local bacteria community has been studied using conventional cultivation method along with modern method of bioindication – a biochip. The low density biochip contains the relevant probes for the identification of the bacterial consortium in soil microbiota. Chemical and microbiological analysis was based on the standards and methodologies developed by International Standards Organizations – ISO and Environmental Protection Agency – EPA. It is prospected that bioremediation can become essential part of remediation against arsenic pollution in the context of circular economy.

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Section: Biochip Design: Dna Probes and Biochip Matrixmentioning

confidence: 99%

Section: Biochip Design: Dna Probes and Biochip Matrixmentioning

confidence: 99%