Study of beta-glucosidase production by wine-related yeasts during alcoholic fermentation. A new rapid fluorimetric method to determine enzymatic activity

Fia, Giovanna; Giovani, Giovanna; Rosi, Iolanda

doi:10.1111/j.1365-2672.2005.02657.x

Cited by 114 publications

(78 citation statements)

References 20 publications

(33 reference statements)

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“…A few reports are available that show glycosidic activity (b-glucosidase) amongst Brettanomyces/ Dekkera strains (Fugelsang et al, 1993;McMahon et al, 1999;Mansfield et al, 2002;Potgieter, 2004;Fia et al, 2005). In these cases, these yeasts may produce wines with enhanced aroma and complexity.…”

Section: Loss Of Colourmentioning

confidence: 99%

Significance of Brettanomyces and Dekkera during Winemaking: A Synoptic Review

Oelofse

Pretorius

Toit

2016

SAJEV

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Wine comprises a complex microbial ecology of opportunistic microorganisms, some of which could potentially induce spoilage and result in consequent economic losses under uncontrolled conditions. Yeasts of the genus Brettanomyces, or its teleomorph Dekkera, have been indicated to affect the chemical composition of the must and wine by producing various metabolites that are detrimental to the organoleptic properties of the final product. These yeasts can persist throughout the harsh winemaking process and have in recent years become a major oenological concern worldwide. This literature review summarises the main research focus areas on yeasts of the genera Brettanomyces and Dekkera in wine. Specific attention is given to the spoilage compounds produced, the methods of detection and isolation from the winemaking environment and the factors for controlling and managing Brettanomyces spoilage.

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Section: Loss Of Colourmentioning

confidence: 99%

Significance of Brettanomyces and Dekkera during Winemaking: A Synoptic Review

Oelofse

Pretorius

Toit

2016

SAJEV

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“…β-Glycosidases activate β-glycosides, secondary metabolites that perform a wide array of cellular functions including the induction of symbiosis, breakdown of endosperm cell wall during germination, phytohormone activation, lignification, the production of aromatic compounds and defense (Nisius 1988;Poulton 1990;Dharmawardhana et al 1995;Leah et al 1995;Hungria & Stacey 1997;Kristoffersen et al 2000;Mizutani et al 2002;Morant et al 2003;Fia et al 2005;Maicas & Mateo 2005;Escamilla-Trevino et al 2006;Halkier & Gershenzon 2006;Lee et al 2006;Suzuki et al 2006;Naoumkina et al 2007;Stauber et al 2012). Some of the defense β-glycosides include α-hydroxynitrile glucosides, benzoxazinoid glycosides, avenacosides, isoflavonoid glycosides and glucosinolates (Niemeyer 1988;Nisius 1988;Poulton 1990;Cairns et al 2000;Halkier & Gershenzon, 2006).…”

Section: Introductionmentioning

confidence: 99%

The heterologous expression of aGlycine maxhomolog of NONEXPRESSOR OF PR1 (NPR1) and α-hydroxynitrile glucosidase suppresses parasitism by the root pathogenMeloidogyne incognitainGossypium hirsutum

Pant

McNeece

Sharma

et al. 2016

Journal of Plant Interactions

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Experiments in Glycine max (soybean) identified the expression of the salicylic acid signaling and defense gene NONEXPRESSOR OF PR1 (NPR1) in root cells (i.e., syncytium) parasitized by the plant parasitic nematode Heterodera glycines undergoing the process of resistance. Gm-NPR1-2 overexpression in G. max effectively suppresses parasitism by H. glycines. The heterologous expression of Gm-NPR1-2 in Gossypium hirsutum impairs the ability of the parasitic nematode Meloidogyne incognita to form root galls, egg sacs, eggs and second-stage juvenile (J2) nematodes.In related experiments, a G. max β-glycosidase (Gm-βg-4) related to Lotus japonicus secreted defense gene α-hydroxynitrile glucosidase LjBGD7 suppresses M. incognita parasitism. The results identify a cumulative negative effect that the transgenes have on M. incognita parasitism and demonstrate that the G. max-H. glycines pathosystem is a useful tool to identify defense genes that function in other agriculturally relevant plant species to plant parasitic nematodes with different strategies of parasitism.ARTICLE HISTORY

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“…In plants, b-glycosidases serve a number of diverse and important functions, including bioactivation of defense compounds (Nisius, 1988;Poulton, 1990;Morant et al, 2003;Halkier and Gershenzon, 2006;Suzuki et al, 2006), cell wall degradation in endosperm during germination (Leah et al, 1995), activation of phytohormones (Kristoffersen et al, 2000;Lee et al, 2006), and lignification (Dharmawardhana et al, 1995;Escamilla-Trevino et al, 2006). In addition, b-glycosidases play key roles in aroma formation in tea, wine, and fruit juice (Mizutani et al, 2002;Fia et al, 2005;Maicas and Mateo, 2005). Plants produce myriad secondary metabolites involved in defense against pathogens and herbivores.…”

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confidence: 99%

The β-Glucosidases Responsible for Bioactivation of Hydroxynitrile Glucosides in Lotus japonicus

et al. 2008

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Lotus japonicus accumulates the hydroxynitrile glucosides lotaustralin, linamarin, and rhodiocyanosides A and D. Upon tissue disruption, the hydroxynitrile glucosides are bioactivated by hydrolysis by specific b-glucosidases. A mixture of two hydroxynitrile glucoside-cleaving b-glucosidases was isolated from L. japonicus leaves and identified by protein sequencing as LjBGD2 and LjBGD4. The isolated hydroxynitrile glucoside-cleaving b-glucosidases preferentially hydrolyzed rhodiocyanoside A and lotaustralin, whereas linamarin was only slowly hydrolyzed, in agreement with measurements of their rate of degradation upon tissue disruption in L. japonicus leaves. Comparative homology modeling predicted that LjBGD2 and LjBGD4 had nearly identical overall topologies and substrate-binding pockets. Heterologous expression of LjBGD2 and LjBGD4 in Arabidopsis (Arabidopsis thaliana) enabled analysis of their individual substrate specificity profiles and confirmed that both LjBGD2 and LjBGD4 preferentially hydrolyze the hydroxynitrile glucosides present in L. japonicus. Phylogenetic analyses revealed a third L. japonicus putative hydroxynitrile glucoside-cleaving b-glucosidase, LjBGD7. Reverse transcriptionpolymerase chain reaction analysis showed that LjBGD2 and LjBGD4 are expressed in aerial parts of young L. japonicus plants, while LjBGD7 is expressed exclusively in roots. The differential expression pattern of LjBGD2, LjBGD4, and LjBGD7 corresponds to the previously observed expression profile for CYP79D3 and CYP79D4, encoding the two cytochromes P450 that catalyze the first committed step in the biosyntheis of hydroxynitrile glucosides in L. japonicus, with CYP79D3 expression in aerial tissues and CYP79D4 expression in roots.

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Study of beta-glucosidase production by wine-related yeasts during alcoholic fermentation. A new rapid fluorimetric method to determine enzymatic activity

Cited by 114 publications

References 20 publications

Significance of Brettanomyces and Dekkera during Winemaking: A Synoptic Review

Significance of Brettanomyces and Dekkera during Winemaking: A Synoptic Review

The heterologous expression of aGlycine maxhomolog of NONEXPRESSOR OF PR1 (NPR1) and α-hydroxynitrile glucosidase suppresses parasitism by the root pathogenMeloidogyne incognitainGossypium hirsutum

The β-Glucosidases Responsible for Bioactivation of Hydroxynitrile Glucosides in Lotus japonicus

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