Study of biofilms of enterobacteria appeared in animals with alimentary diseases

Lenchenko, Ekaterina; Blumenkrants, Dmitry

doi:10.30896/0042-4846.2020.23.1.25-29

Cited by 2 publications

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“…This pathology is characterized by a polymorphism of clinical manifestations, associated not so much with the epidemic or epizootic situation, but with the age and state of the patient's defenses. Opportunistic enterobacteria, under certain conditions, also play an active role in the etiology of acute intestinal infections in humans and animals [5,6,7,8,12].…”

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confidence: 99%

Persistence factors and antibiotic susceptibility of enterobacteria isolated from various animal species

Ermakov

Titov²

2022

BIO Web Conf.

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During the study, we isolated 575 strains of enterobacteria from various animal species belonging to the genus Escherichia, Shigella, Salmonella, Klebsiella, Proteus, Providencia, Hafnia, Morganella, Enterobacter, Citrobacter, Serratia, Erwinia, Kluyvera, Yersinia. In farm animals, the amount of transient pathogenic enterobacteria Salmonella enteritidis and Yersinia enterocolitica did not exceed 0.22-0.12%. Among wild animals, the proportion of pathogenic Salmonella enteritidis and Yersinia enterocolitica did not exceed 0.24-0.11%, while Shigella dysenteriae and Shigella flexneri were isolated at a low concentration of 0.01%. In the feces of zoo animals, the amount of Salmonella enteritidis and Yersinia enterocolitica did not exceed 0.21-0.10%, while Shigella dysenteriae and Shigella flexneri were detected at the level of 0.01% of the total concentration of enterobacteria. The share of Salmonella enteritidis and Yersinia enterocolitica in domestic animals did not exceed 0.012-0.04%. Persistence factors in Salmonella enteritidis, Shigella dysenteriae and Shigella flexneri, Klebsiella oxytoca, and Yersinia enterocolitica were observed to be the highest among all isolated enterobacteria. Enterobacteria showed high resistance to benzylpenicillin from the group of natural penicillins to streptomycin, cephalothin from the group of cephalosporins of the first generation, polymyxin B, ofloxacin (tarivid), and metronidazole. Carbenicillin from the group of carboxypenicillins and piperacillin from the group of ureidopenicillins, kanamycin, amikacin, and gentamicin, cefepime from the group of IV generation cephalosporins, tetracycline, doxycycline and chloramphenicol, nalidixic acid, trimethoprim showed the highest antimicrobial activity against all cultures of enterobacteria isolated in this study.

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Section: Introductionmentioning

confidence: 99%

Persistence factors and antibiotic susceptibility of enterobacteria isolated from various animal species

Ermakov

Titov²

2022

BIO Web Conf.

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Studies of biofilms and phenotypic characteristics of Candida fungi

Lenchenko

Sachivkina

2020

Veterinariâ segodnâ

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Yeast-like fungi of the genus Candida are causative agents of the infectious pathology of the mucous membrane of the gastrointestinal, respiratory, urogenital tracts and skin of mammals, sepsis, and disseminated infection in birds. The search and testing of multilevel algorithms for biofilm identification when exposed to chemotherapeutic and disinfectant drugs for blocking the synthesis or destruction of the intercellular matrix in the development of superficial, deep and systemic candidiasis of animals are relevant for developing and improving diagnostic and antiepidemic measures. It was established that the formation of biofilm heterogeneous structure comprises multiple stages implementing the processes of intercellular communication due to the synthesis of a polymer matrix composites. Optical microscopy revealed a three-dimensional structure of biofilms in the form of a dense network consisting of yeast cells, hyphal and pseudohyphalic forms surrounded by an intercellular polymer matrix. Candida spp. pathogenicity factors contribute to infection of susceptible species due to adhesion, invasion, secretion of hydrolases, dimorphism. Formation of mono-species or poly-species biofilms of microorganisms, including Candida spp., causes the development of superficial, deep and systemic candidiasis. Detection of a large amount of yeast and micellar phases in C. albicans and C. africana isolates was a differential sign of a significant degree of colonization of the mucous membranes of the larynx, pharynx, and tonsils in case of local and systemic pathologies in pigs. The results of studies of the biofilm heterogeneous structure and phenotypic signs of yeast-like fungi can be used in a comparative study of biological characteristics and the identification of common patterns and differential signs of microorganisms, optimization of mycological diagnostics, and also in the development of antimycotic drugs.

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Study of biofilms of enterobacteria appeared in animals with alimentary diseases

Cited by 2 publications

References 0 publications

Persistence factors and antibiotic susceptibility of enterobacteria isolated from various animal species

Persistence factors and antibiotic susceptibility of enterobacteria isolated from various animal species

Studies of biofilms and phenotypic characteristics of Candida fungi

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