Study of Ca2+, Mn2+ and Cu2+ binding to DNA in solution by means of IR spectroscopy

Hackl, Elene V.; Kornilova, S. V.; Kapinos, Larisa E.; Andrushchenko, Valery; Galkin, V. L.; Grigoriev, D.N.; Blagoı̆, Yu. P.

doi:10.1016/s0022-2860(96)09534-8

Cited by 69 publications

(75 citation statements)

References 3 publications

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“…3 These highly active species may further attack the DNA molecule, usually at thymine (T) or guanine (G) residues. 16,17 Combining the results of the electrochemical characterization of the DNA-Cu-DA system, we may as well explain why the DNA-Cu-DA system showed the fastest reaction rates among these three-component systems. The Cu ion may react with DNA to form a Cu-DNA complex.…”

Section: Discussionmentioning

confidence: 73%

Spectroscopic and Electrochemical Studies of DNA Breakage Induced by Dopamine and Copper Ion

Li¹,

et al. 2003

ANAL. SCI.

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Section: Discussionmentioning

confidence: 73%

Spectroscopic and Electrochemical Studies of DNA Breakage Induced by Dopamine and Copper Ion

Li¹,

et al. 2003

ANAL. SCI.

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“…Figure 6(a) and 6(b) showed the IR spectra of DNA-Co 2þ biomatrix (R ¼ 0:5) under 0% RH and 100% RH conditions, respectively. The absorption band at 1250-1500 cm À1 , which can be attributed to the nucleic acid base, 18 relatively increased with the humidity. This is due to the interaction of nucleic acid base with the hydrated-cobalt ion.…”

Section: Resultsmentioning

confidence: 98%

“…The increase of intensity based on the interaction with DNA and metal ion has been reported at the IR spectra in aqueous solution. 13,15,18 Additionally, the conformational change of DNA structure with the RH has been reported. [19][20][21] In this case, DNA structure changes from A-form to B-form with the increase of RH.…”

Section: Resultsmentioning

confidence: 99%

Utilization of DNA-metal Ion Biomatrix as a Relative Humidity Sensor

Yamada

Sugiyama

2008

Polym J

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Recently, we prepared the novel biomatrix by the mixing of DNA and metal ion. These biomatrices have a metal ion property, such as oxidative and reducible effects, catalytic action, or the change of coordination number. So, we proposed the humidity sensor for the novel utilization of DNA-metal ion biomatrix. The biomatrix with the humidity property was prepared by the mixing of DNA and metal ion, such as cobalt (II) or nickel (II) ions. These matrices indicated the different color with the change of relative humidity (RH). Especially, the color of Co 2þ -containing biomatrix changed from blue to red with the increase of RH. These biomatrices may have a potential for the novel RH sensor with flexibility, low cost, nonhazardous, and environmentally benign.KEY WORDS: DNA / Biopolymers / Matrix / Humidity Sensor / Composites / DNA, one of the most important materials for the genetic process, can easily be purified from either salmon milts or shellfish gonads, which are generally discarded as industrial waste around the world. In addition, double-stranded DNA has highly specific functions, 1,2 such as the accumulation of intercalating compounds, the complementary interaction between nucleic acid bases, and the interaction with metal ions. Therefore, the double stranded DNA has been attracted for novel functional materials, such as adsorbents of harmful compounds, 3,4 electro-and ion-conductive materials, 5,6 and functional materials. [7][8][9] We could also convert the discarded DNA into a novel bio-matrix by the hybridization of DNA and metal ions.10 These DNA-metal ion matrices could be created in various desirable forms, such as a gel, capsule, film, or fiber.10 Additionally, DNA-metal ion biomatrix indicated the oxidative property.10 Metal ions have many functions, such as reductive effect, catalytic action, or the change of coordination number. Therefore, DNA-metal ion biomatrices might be used for various functional materials.Cobalt (II) and nickel (II) ions can indicate the different color by the change of coordination number with water molecules 11,12 and this property has been used for the silica gel with the moisture indicator. However, this material is rigid and non-flexible, as a result, the utilized-fields for the moisture indicator or humidity sensor have been limited. So, we propose the relative humidity (RH) sensor for the novel utilization of DNA-metal ion biomatrix that is flexible, non-hazardous, biodegradable, and environmentally benign. These RH sensors with the DNA are novel engineering material and have not been reported as far as we know.In this paper, we prepared DNA-metal ion biomatrix by the mixing of double-stranded DNA and metal ion, such as cobalt (II) and nickel (II) ions. This matrix had been constructed by the electrostatic interaction between the phosphate group of DNA and metal ion. This DNA-metal ion biomatrix indicated the color change with the RH. Additionally, these color changes is quickly and reversible. Furthermore, the similar phenomena were also obtained at the biomatrix co...

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“…Met 2+ -induced DNA condensation in our experiments included both intra-and inter-molecular interactions. In the compact state DNA remained in the B-conformation limits [1,2]. A further study allowed us to suggest that the mechanism of DNA condensation under Met 2+ ion action is not completely electrostatic but involves a partial destabilisation of DNA due to Met 2+ ion interaction with DNA bases [3,4].…”

mentioning

confidence: 99%

DNA interaction with biologically active divalent metal ions: binding constants calculation

Hackl

Galkin

Blagoı̆

2004

International Journal of Biological Macromolecules

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Study of Ca2+, Mn2+ and Cu2+ binding to DNA in solution by means of IR spectroscopy

Cited by 69 publications

References 3 publications

Spectroscopic and Electrochemical Studies of DNA Breakage Induced by Dopamine and Copper Ion

Spectroscopic and Electrochemical Studies of DNA Breakage Induced by Dopamine and Copper Ion

Utilization of DNA-metal Ion Biomatrix as a Relative Humidity Sensor

DNA interaction with biologically active divalent metal ions: binding constants calculation

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