Study of enzyme sensors with wide, adjustable measurement ranges for in-situ monitoring of biotechnological processes

Mross, Stefan; Zimmermann, Tom; Zenzes, Sebastian; Kraft, Michaël; Vogt, H.

doi:10.1016/j.snb.2016.10.054

Cited by 7 publications

(5 citation statements)

References 22 publications

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“…S6, ESI†). 44 Satisfyingly, a positive linear relationship was obtained between the fluorescence ratio (( F − F 0 )/ F 0 ) and the ATP concentration value (Fig. 4d).…”

Section: Resultsmentioning

confidence: 60%

Nitrogen-doped graphene quantum dot-based portable fluorescent sensors for the sensitive detection of Fe³⁺ and ATP with logic gate operation

et al. 2023

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“…S6, ESI†). 44 Satisfyingly, a positive linear relationship was obtained between the fluorescence ratio (( F − F 0 )/ F 0 ) and the ATP concentration value (Fig. 4d).…”

Section: Resultsmentioning

confidence: 60%

Nitrogen-doped graphene quantum dot-based portable fluorescent sensors for the sensitive detection of Fe³⁺ and ATP with logic gate operation

et al. 2023

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“…There were no significant differences (p> 0.05) observed at glucose concentrations of 200 mg/dL and 400 mg/dL, as shown in Figure 7a. Previous studies involving enzymatic glucose and lactate sensors sterilized with the same method exhibited functional sensors but with sensitivity reductions up to 60% after radiation 28,29 . Dang et al observed that when the optical enzymatic sensors were stored in a 5 mM TRIS buffer during radiation, protective effects on the proteins were observed, resulting in an 80% retention of response when exposed to 15kGy 30 .…”

Section: Sterilization and Reproducibilitymentioning

confidence: 97%

Continuous Monitoring of Glucose and Oxygen using an Insertable Biomaterial-based Multianalyte Barcode Sensor

Pradhan,

Chimene,

et al. 2024

Preprint

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Chronic diseases including diabetes, cardiovascular diseases, and microvascular complications contribute significantly to global morbidity and mortality. Multiplexing technologies offer a promising approach for the simultaneous detection and management of comorbidities, providing comprehensive disease insights. In this work, we describe a miniaturized optical barcode sensor with high biocompatibility for continuous monitoring of glucose and oxygen. This enzymatic sensor relies on oxygen consumption in proportion to local glucose levels and the phosphorescence reporting of tissue oxygen with a lifetime-based probe. The sensor was designed to operate in a tissue environment with low levels of dissolved oxygen. The barcode sensor consists of a poly(ethylene) glycol diacrylate (PEGDA) hydrogel with four discrete compartments separately filled with glucose or oxygen-sensing phosphorescent microparticles. We evaluated the response of the barcode hydrogels to fluctuating glucose levels over the physiological range under low oxygen conditions, demonstrating controlled tuning of dynamic range and sensitivity. Moreover, the barcode sensor exhibited remarkable storage stability over 12 weeks, along with full reversibility and excellent reproducibility (~6% variability in phosphorescence lifetime). Electron beam sterilization had a negligible impact on the glucose response of the barcode sensors. Furthermore, our investigation revealed minimal phosphorescence lifetime changes in oxygen compartments while exhibiting increased lifetime in glucose-responsive compartments when subjected to alternating glucose concentrations (0 and 200 mg/dL), showcasing the sensor's multianalyte sensing capabilities without crosstalk between compartments. Additionally, evaluation of tissue response to sensors inserted in pigs revealed appropriate biocompatibility of the barcodes.

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“…glucose and lactate), a three-electrode configuration with a working electrode (WE), counter electrode (CE), and reference electrode (RE) was adopted. These electrodes were arranged in concentric circles to ensure even distribution of the current density between the WE and CE, and independence of the direction flow in stirred solution [22]. Furthermore, this circular shape makes it easier to dispense the enzyme solution onto the WE area (see Fig.…”

Section: A Enzyme Sensorsmentioning

confidence: 99%

“…Amperometry is an electrochemical technique of analysis in which the potential applied to the measuring cell is kept constant while the response current depends on the concentration of the electroactive particles in the cell. The operation of these amperometric biosensors is based on selective enzymatic conversion of glucose or lactate molecules (in presence of oxygen -a cosubstrate of the enzymatic reaction) by an enzyme which results in generation of electrochemically active hydrogen peroxide (H2O2), subsequent oxidation of the generated H2O2 yields two electrons at positive potential, and detection of these electrons can be registered as an oxidation current proportional to substrate concentration by a potentiostat [1], [22], [23]. The corresponding reactions for the enzymes glucose oxidase and lactate oxidase are given in (1) and (2), respectively.…”

Section: A Enzyme Sensorsmentioning

confidence: 99%

Multi-Sensor Chip for Monitoring Key Parameters in Bioprocesses

et al. 2021

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This paper presents a feasibility study on the integration of multiple biosensors based on amperometric, potentiometric, and impedimetric detection techniques, as well as physical sensors on a single chip. The sensor chip comprises six sensors for in-situ monitoring of important process parameters in a bioprocess: glucose, lactate, pH, cell density, dissolved oxygen concentration, and temperature. The waferlevel parallel fabrication process of multiple microsensors on a single substrate involves four relatively simple processes. The fabricated enzyme sensors were tested with a linear working range up to a concentration of 10 mM for the glucose and lactate sensors and show a fast response time of 20 s. The integrated pH sensor was governed by a near-Nernstian equation with a sensitivity of 60.8 ± 2.4 mV/pH. Cell density, dissolved oxygen, and temperature sensor were also electrically tested. Results obtained from experimental measurements can be considered a promising step towards the realization of a real-time and parallel in-situ measurement platform for bioprocesses.

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Study of enzyme sensors with wide, adjustable measurement ranges for in-situ monitoring of biotechnological processes

Cited by 7 publications

References 22 publications

Nitrogen-doped graphene quantum dot-based portable fluorescent sensors for the sensitive detection of Fe³⁺ and ATP with logic gate operation

Nitrogen-doped graphene quantum dot-based portable fluorescent sensors for the sensitive detection of Fe³⁺ and ATP with logic gate operation

Continuous Monitoring of Glucose and Oxygen using an Insertable Biomaterial-based Multianalyte Barcode Sensor

Multi-Sensor Chip for Monitoring Key Parameters in Bioprocesses

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Study of enzyme sensors with wide, adjustable measurement ranges for in-situ monitoring of biotechnological processes

Cited by 7 publications

References 22 publications

Nitrogen-doped graphene quantum dot-based portable fluorescent sensors for the sensitive detection of Fe3+ and ATP with logic gate operation

Nitrogen-doped graphene quantum dot-based portable fluorescent sensors for the sensitive detection of Fe3+ and ATP with logic gate operation

Continuous Monitoring of Glucose and Oxygen using an Insertable Biomaterial-based Multianalyte Barcode Sensor

Multi-Sensor Chip for Monitoring Key Parameters in Bioprocesses

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Product

Resources

About

Nitrogen-doped graphene quantum dot-based portable fluorescent sensors for the sensitive detection of Fe³⁺ and ATP with logic gate operation

Nitrogen-doped graphene quantum dot-based portable fluorescent sensors for the sensitive detection of Fe³⁺ and ATP with logic gate operation