Study of Epilobium angustifolium L. amino acids content by HPLC method

Abstract

The use of plant raw materials is one of the areas of modern pharmaceutical science in the production of herbal drugs. The genus Epilobium counts more than 200 species, many species of which are used in traditional medicine. Among the Epilobium species, Epilobium angustifolium is one of the well-known medicinal plants which have been used worldwide in habitual medicine. There is insufficient information in the literature on the biologically active substances of Epilobium angustifolium L. The presence of three major polyphenol groups: phenolic acids, flavonoids, and ellagitannins were identified in E. angustifolium extracts. Traditionally, the infusion of leaves of this plant could be useful for headaches, cold and gastrointestinal disorder. The Epilobium angustifolium L. as an insufficiently studied plant is a promising object of study, including amino acids composition. To assess the relationship between the production of primary metabolites and their possible therapeutic properties, we analyzed the amino acid profile of the plant Epilobium angustifolium used in traditional medicine. The study of compounds generated by plants as a result of defense mechanisms permits an understanding of the molecular mechanism involved in their medicinal properties.
 The aim. Thus, the aim of the study was to conduct an HPLC analysis of the amino acids of E. angustifolium to establish the prospects for the use of the raw materials in medical and pharmaceutical practice. The results of the current study will be used in further breeding programs aimed to obtain an industrial form of E. angustifolium suitable for pharmaceutical and food applications.
 Materials and methods. The determination of amino acids composition of Epilobium angustifolium was conducted using Agilent 1200 (Agilent Technologies, USA).
 Results. The HPLC method identified sixteen free amino acids and seventeen bound amino acids in the Epilobium angustifolium herb. The studies have shown that Epilobium angustifolium L. herb is mainly composed of free amino acids such as L-phenylalanine (1.65 µg/mg), L-glutamic acid (1.51 µg/mg), L-arginine (1.24 µg/mg), L-alanine (0.98 µg/mg) and L-aspartic acid (0.57 µg/mg), which were presents in the greatest amount. The dominant bound amino acids in the studied raw material were L-glutamic acid, L-aspartic acid, L-leucine, and L-alanine, the content of which was 32.37 µg/mg, 10.59 µg/mg, 8.70 µg/mg, and 6.22 µg/mg respectively.
 Conclusions. Using the HPLC method determined the amino acids in the herb of Epilobium angustifolium L. The concentrations of L-aspartic acid, L-glutamic acid, L-arginine, L-alanine and L-phenylalanine are predominate among free and bound amino acids in the Epilobium angustifolium L. herb. The result shows that Epilobium angustifolium L. is the source of amino acids, so the use of this plant raw material for new remedies is possible in the future

Investigation of amino acids content in the herb and tubers of Stachys sieboldii

“…program and are shown in Table 2. All statistical tests were performed at a confidence level of 95% and k = 2 (Feshchenko et al 2021b). Linearity testing was repeated with the same samples after a complete restart of the system with removement and re-installation of the column.…”

“…The quantitative content of amino acids is calculated from the value of the of the peak area of the amino acids. The content of bound amino acids was determined by subtracting the content of free amino acids from their total content (Feshchenko et al 2021b).…”

Quantitative analysis of fatty acids and monosaccharides composition in Chamerion angustifolium L. by GC/MS method

“…The aboveground part of C angustifolium was collected during flowering period in the Ternopil region (Ukraine) in 2017 (Feshchenko 2021b). The raw material was authenticated by prof. Svitlana Marchyshyn (TNMU, Ternopil, Ukraine).…”

“…C. angustifolium has long been used in folk medicine as a sedative, antimicrobial, adstringent, and analgesic, wound healing, anti-inflammatory, diaphoretic, emollient and antisclerotic agent (Safonov 2008;Feshchenko et al 2021a). Also, it is used in the traditional medicine of various countries around the world (Schepetkin et al 2016;Feshchenko et al 2021b).…”