Study of Ethyl Acetate Crude Extract of Lasiodiplodia pseudotheobromae IBRL OS-64, an Endophytic Fungus Isolated from the Leaf of Ocimum sanctum for Anti-Biofilm Agent (Funded Work)

Taufiq, Muhammad; Darah, I.

doi:10.22376/ijpbs.2020.11.2.b7-18

Cited by 3 publications

(7 citation statements)

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“…The inoculum of MRSA ATCC 33591 was prepared according to Taufiq and Darah ( 14 ). The bacterial strain was cultured in Mueller-Hinton agar (MHA) and incubated for 24 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

“…The anti-biofilm activity of MRSA ATCC 33591 was performed using a semi-quantitative adherence assay method by Taufiq and Darah ( 14 ). The plates were air-dried overnight and the OD of each well was measured at 570 nm and recorded.…”

Section: Methodsmentioning

confidence: 99%

“…The inhibition assay of the initial and preformed biofilm was performed according to the method described by Taufiq and Darah ( 14 ) in sterilised flat-bottom 96-well microtitre plates.…”

Section: Methodsmentioning

confidence: 99%

“…The quantification of bacterial biofilm was determined according to the procedures described by Taufiq and Darah ( 14 ). All experiments were carried out in triplicate.…”

Section: Methodsmentioning

confidence: 99%

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Fungal Extract of Lasiodiplodia pseudotheobromae IBRL OS-64 Inhibits the Growth of Skin Pathogenic Bacterium and Attenuates Biofilms of Methicillin-Resistant Staphylococcus aureus

Jalil

Demirtaş

2021

MJMS

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Background: The emergence of multidrug-resistant pathogens associated with biofilm formation can cause life-threatening infections to humans. Therefore, the present study aims to evaluate the effects of the fungal extract of Lasiodiplodia pseudotheobromae (L. pseudotheobromae) Industrial Biotechnology Research Laboratory (IBRL) OS-64 on bacterial cells and the biofilm formation of methicillin-resistant Staphylococcus aureus (MRSA). Methods: Broth microdilution and semi-quantitative adherence assays were conducted to determine the anti-biofilm activity of the fungal extract. Light and scanning electron microscopy (SEM) analyses were performed to observe the effect of the fungal extract on biofilm formation by MRSA. Results: The transmission electron microscopy (TEM) microphotographs showed that the bacterial cells were severely damaged upon 24 h exposure to the extract and displayed several symptoms such as cell shrinkage and breakage. Meanwhile, results from the antibiofilm study indicated the extract attenuated the initial and preformed biofilms of MRSA by 80.82% and 61.39%, respectively. The initial biofilm was more sensitive to the extract compared to the pre-formed biofilm, as evidenced by the light microscopy and SEM observations that demonstrated more severe bacterial cell damage on the initial biofilms compared to pre-formed biofilms. Conclusion: The ethyl acetate extract of L. pseudotheobromae IBRL OS-64 significantly inhibited bacterial cells growth and eliminated biofilm formation by MRSA.

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“…The inoculum of MRSA ATCC 33591 was prepared according to Taufiq and Darah ( 14 ). The bacterial strain was cultured in Mueller-Hinton agar (MHA) and incubated for 24 h at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The inhibition assay of the initial and preformed biofilm was performed according to the method described by Taufiq and Darah ( 14 ) in sterilised flat-bottom 96-well microtitre plates.…”

Section: Methodsmentioning

confidence: 99%

“…The quantification of bacterial biofilm was determined according to the procedures described by Taufiq and Darah ( 14 ). All experiments were carried out in triplicate.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Fungal Extract of Lasiodiplodia pseudotheobromae IBRL OS-64 Inhibits the Growth of Skin Pathogenic Bacterium and Attenuates Biofilms of Methicillin-Resistant Staphylococcus aureus

Jalil

Demirtaş

2021

MJMS

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show abstract

“…The inhibition assay of the initial/pre-formed biofilm and quantification of bacterial biofilm were performed according to the method described by Taufiq and Darah [21]. All experiments were carried out in triplicate.…”

Section: Inhibition Assay Of the Initial And Preformed Biofilmmentioning

confidence: 99%

Antibacterial and Antibiofilm Activities of Crude Extract of Lasiodiplodia pseudotheobromae IBRL OS-64 against Foodborne Bacterium, Yersinia enterocolitica

Taufiq

Demirtaş

2020

JPRI

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The antibacterial and antibiofilm activities of crude extract of Lasiodiplodia pseudotheobromae IBRL OS-64 was studied and tested against a foodborne pathogenic bacterium, Yersinia enterocolitica. The ethyl acetate extract exhibited favorable antibacterial activity with the zone of inhibition was 20.3±0.6 mm compared to dichloromethane (15.0±0.3 mm) and butanol (9.0±0.3 mm) extracts. Minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) values of the extract were 125 and 250 µg/mL, respectively. Structural degeneration studies through scanning electron microscopy (SEM) and transmission electron microscope (TEM) micrographs exhibited major abnormalities that occurred on the bacterial cells after exposure to the extract were complete alterations in their morphology and collapsed of the cells beyond repair. The findings showed that the extract possesses antibiofilm activity against the initial and preformed biofilm of Y. enterocolitica with the highest inhibition value of 69.12% and 58.70%, respectively. The results also revealed the initial biofilm was more susceptible to the extract as compared to pre-formed biofilm. The light microscopy (LM) and SEM photomicrographs proved that the fungal extract significantly eliminates extracellular polysaccharide (EPS) matrices and hinder the attachment of the bacterial cells for biofilm formation. Therefore, the current study suggested the ethyl acetate crude extract from an endophytic fungus, L. pseudotheobromae IBRL OS-64 may be an effective antibacterial and anti-biofilm agent to treat foodborne pathogens.

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Development of Antibiofilm Substances by Endophytic Microorganisms with an Emphasis on Medicine

Rodrigues¹,

Assis²,

Foggi³

et al. 2022

Focus on Bacterial Biofilms

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The growing antimicrobial resistance and persistence of pathogenic microorganisms in infections–particularly in nosocomial infections–have become a major problem for public health worldwide. One of the main causes of these issues is the formation of biofilms, which are microbial communities associated with extracellular polymeric substances (EPS) that form a slimy extracellular matrix, causing the bacteria to become more tolerant to usual drugs in these structures. Thus, the search for new antibiofilm compounds is part of a strategy to deal with this problem. Endophytic microorganisms such as bacteria and fungi, mutualistically associated with plants, are sources of compounds with biological properties, including antimicrobials, and can be important allies in the synthesis of antibiofilm. These secondary metabolites can interfere with cell-to-cell communication and cell adhesion ability, promoting the dispersal of bacterial colonies and affecting biofilm. Since endophytes are cultivable in laboratory conditions, these microorganisms are environmentally friendly, as they do not contribute to pollution, are easy to handle and are produced on a large scale. Furthermore, metabolites from endophytes are of natural origin and may contribute to the reduced use of synthetic drugs. Considering these aspects, this chapter will focus on the characterization of endophytic microorganisms as potential active sources of antibiofilm and antimicrobial compounds with applications in medicine.

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Study of Ethyl Acetate Crude Extract of Lasiodiplodia pseudotheobromae IBRL OS-64, an Endophytic Fungus Isolated from the Leaf of Ocimum sanctum for Anti-Biofilm Agent (Funded Work)

Cited by 3 publications

References 0 publications

Fungal Extract of Lasiodiplodia pseudotheobromae IBRL OS-64 Inhibits the Growth of Skin Pathogenic Bacterium and Attenuates Biofilms of Methicillin-Resistant Staphylococcus aureus

Fungal Extract of Lasiodiplodia pseudotheobromae IBRL OS-64 Inhibits the Growth of Skin Pathogenic Bacterium and Attenuates Biofilms of Methicillin-Resistant Staphylococcus aureus

Antibacterial and Antibiofilm Activities of Crude Extract of Lasiodiplodia pseudotheobromae IBRL OS-64 against Foodborne Bacterium, Yersinia enterocolitica

Development of Antibiofilm Substances by Endophytic Microorganisms with an Emphasis on Medicine

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