Nanowire ACF is fabricated by template synthesis method based on electrochemical deposition. Due to the high aspect ratio of length to diameter (50:1), the nanowires can act as compliant conductors to electrically connect the bumpless dies with a small bonding force. In this work, an interconnection resistance of 90 mΩ per 40 × 40 µm 2 pad is measured for the Cu NW-ACF bonding between two Au bumpless dies. An increasing leakage current up to 10 -6 A in x-y plane is observed with the elevated voltage till 20 V for a pitch size of 80 µm. A comparison study of NW-ACF to conventional particle based ACF is carried out. The bonding mechanisms of the NW-ACF bonding to bumped and bumpless dies and its difference to particle ACF bonding is revealed by bonding interface analysis.