The focus of this paper is to study the feeding preference and impact of woody species on African elephants in Babile Elephant Sanctuary (BES), Eastern Ethiopia. Data were collected through purposive random and systematic sampling techniques. Seven sites from three different habitats in the sanctuary were selected by considering the availability of dung and consumed plant parts. The result showed, among 38 species browsed by elephants, 24(63.15%) were the most preferred elephant diet. Besides, from dissecting 75 dung boli of 24 plant species (yielding 2841seeds); 2009 (70%) of seeds were fed by elephants during the wet season. And, the seeds were analysed using the focal watch and indirect methods. Woody species, about 23(76.7%) in the riverine, 36 (68%) in woodland, and 19(54.3%) in bushland habitats were mainly impacted on parts of primary/secondary branches and main stem. Among these, a small size class of trees was more easily damaged by elephants. Based on research results, the following inference is drawn: identifying and documenting the dietary composition, feeding preference, and impact of elephants on woody species in BES is important to build gaps in knowledge for conservationists to design plans for restoring the species and sustaining elephants existence in the sanctuary.