Study of genetic diversity in Mycobacterium tuberculosis by using mycobacterial interspersed repetitive unit: Variable number tandem repeat typing in Khuzestan Province, Iran

Vatani, Shideh; Khosravi, Azar Dokht; Feizabadi, Mohammad Mehdi; Jolodar, Abbas

doi:10.5897/ajmr11.206

Cited by 8 publications

(3 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This information showed the most similarity of Sistan-Baluchestan province with isolates of Hormozgan that can explain the possibility of transmission of MTB from other provinces especially this province and vice versa [27]. There was no similarity between the patterns found in this study and those formerly reported from East Azerbaijan province and Khuzestan province [35, 36]. Moreover the MIRU-VNTR profiles did not match any strain in the MIRU-VNTRplus database and were debated unrelated to reference strains.…”

Section: Discussionmentioning

confidence: 51%

Genotyping of Mycobacterium tuberculosis Isolates from Hormozgan Province of Iran Based on 15-Locus MIRU-VNTR and Spoligotyping

Zamani

Haeili

Nasiri

et al. 2016

International Journal of Bacteriology

View full text Add to dashboard Cite

Background. Considering that Hormozgan province in Iran (southern part of Iran on the Persian Gulf) is among the areas with high prevalence of MDR-MTB and attracts so many sailors and tourists, genetic diversity of MTB isolates circulating in this part of Iran was evaluated. Pattern of TB transmission was also examined. Methods and Material. A total of 38 isolates of MTB were cultured from TB patients from Hormozgan province of Iran and standard MIRU-VNTR typing and spoligotyping were applied to genotype these isolates. Drug susceptibility testing was performed using proportion method. Results. There were 28 VNTR profiles comprising 5 clusters and 23 unique isolates compared to 21 spoligotyping profiles, which contained 9 clusters and 12 unique isolates. Latin American-Mediterranean (n = 9, 23.6%) was found to be the most predominant lineage. MIRU-VNTR analysis, with an HGDI of 0.975, was more discriminating than spoligotyping, which had an HGDI of 0.955. The estimated proportion of TB cases due to recent transmission was 26.3% and 44.7% by MIRU-VNTR and spoligotyping, respectively. The rates of monodrug resistance and MDR were 15.8% and 7.9%, respectively. Two of 3 MDR strains were found to be related to MIRU-VNTR and belonged to the same spoligotyping cluster characterized with T1/SIT53 genotype. Conclusions. The high genetic diversity among MTB isolates suggests that transmission occurred from different sources to this area. Reactivation of a priori, latent MTB infection was found to contribute mainly to TB cases in this geographic region.

show abstract

Section: Discussionmentioning

confidence: 51%

Genotyping of Mycobacterium tuberculosis Isolates from Hormozgan Province of Iran Based on 15-Locus MIRU-VNTR and Spoligotyping

Zamani

Haeili

Nasiri

et al. 2016

International Journal of Bacteriology

View full text Add to dashboard Cite

show abstract

“…Among MIRU loci in hospitals of Paris, France, locus 40 (h = 0.74) showed the highest variation (9). In three provinces in Iran, locus 16 (h = 0.64) exhibited the greatest variation (21), whereas in the province of Khuzestan in Iran, locus 31 (h = 0.73) displayed the highest variation (22). In a genotyping study of M. tuberculosis in China, locus C (h = 0.54) had the highest variation among ETR loci (23).…”

Section: Discussionmentioning

confidence: 98%

“…In a study of TB cases in Singapore, Sun et al (25) reported a discriminatory power of 0.994 for 68 cases. Studies of TB cases performed in Khoozestan province in Iran, Taiwan, and Samara in Russia reported a discriminatory power of 0.991 for 61 cases, 0.972 for 502 cases, and 0.625 for 129 cases, respectively (22, 24, 26). As the study in Singapore was based on a small number of isolates, its discriminatory power was slightly lower than in the present study.…”

Section: Discussionmentioning

confidence: 99%

Molecular Diversity of Mycobacterium tuberculosis Strains in Northwestern Iran

Pourostadi

Rashedi

Poor

et al. 2016

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: Years after the development of antituberculosis (TB) drugs, many people continue to suffer from this disease. To control the spread of TB, strains of the Mycobacterium tuberculosis complex need to be determined, and sources of infection must be identified. Such steps should help to prevent transmission of the infection. Objectives: The aim of this study was to perform molecular genotyping of isolates of the M. tuberculosis complex obtained from patients in northwestern Iran. Methods: One hundred ninety-four culture-positive M. tuberculosis isolates obtained from patients in northwestern Iran were analyzed using the mycobacterial interspersed repetitive unit-exact tandem repeats (MIRU-ETR) method. Results: The MIRU-ETR method distinguished 162 different patterns in the 194 isolates, comprising 23 clusters and 139 unique patterns. Its discriminatory power according to the Hunter-Gaston discriminatory index (HGDI) was 0.9978. The largest cluster contained six isolates. Conclusions: This research indicated that various strains of M. tuberculosis were responsible for TB and that the majority of cases were due to reactivation.

show abstract

Genetic diversity of multidrug‐resistant Mycobacterium tuberculosis strains isolated from tuberculosis patients in Iran using MIRU‐VNTR technique

Khosravi

Shahraki

Dezfuli

et al. 2017

The Kaohsiung J of Med Scie

View full text Add to dashboard Cite

Tuberculosis (TB) is considered as one of the most important infectious diseases in the world, and recent rise and spread of multidrug-resistant (MDR) Mycobacterium tuberculosis (MTB) strains, have made the matter worsened. Due to the importance of TB prevalence in Iran, this study was designed to investigate the genetic diversity among MDR strains of MTB by MIRU-VNTR typing scheme. A total of 88 drug resistant M. tuberculosis isolates belong to pulmonary TB cases were collected from several TB reference centers of Iran. Drug susceptibility testing for Isoniazid and Rifampin was performed using the agar proportion method and MDR isolates were underwent genotyping by using 12-locus- based MIRU-VNTR typing. On performing proportion method, 22 isolates were identified as MDR. By typing of MDR isolates using 12-loci MIRU-VNTR technique, high diversity were demonstrated in MDR strains and these were classified into 20 distinct MIRU-VNTR genotypes. MIRU loci 10 and 26 were the most discriminatory loci with 8 and 7 alleles respectively; while MIRU loci 2, 20, 24 and 39 were found to be the least discriminatory with 1-2 alleles each. We noticed a mixed infection in isolate 53, as this isolate comprised simultaneous two alleles in MIRU loci 40, 10, 16 and 39. In conclusion, this result represents MIRU-VNTR typing as a useful tool for studying genetic diversity of MDR-MTB in regional settings, and will help the health sectors to construct a preventive program for MDR-TB. Additionally, it can detect mixed infection which can facilitate management of treatment.

show abstract

Study of genetic diversity in Mycobacterium tuberculosis by using mycobacterial interspersed repetitive unit: Variable number tandem repeat typing in Khuzestan Province, Iran

Cited by 8 publications

References 35 publications

Genotyping of Mycobacterium tuberculosis Isolates from Hormozgan Province of Iran Based on 15-Locus MIRU-VNTR and Spoligotyping

Genotyping of Mycobacterium tuberculosis Isolates from Hormozgan Province of Iran Based on 15-Locus MIRU-VNTR and Spoligotyping

Molecular Diversity of Mycobacterium tuberculosis Strains in Northwestern Iran

Genetic diversity of multidrug‐resistant Mycobacterium tuberculosis strains isolated from tuberculosis patients in Iran using MIRU‐VNTR technique

Contact Info

Product

Resources

About