Study of HIV-1 subtypes in serodiscordant couples attending an integrated counselling and testing centre in Mumbai using heteroduplex mobility analysis and DNA sequencing

Mehta, PR; Shashwati, Nema; Paranjpe, Supriya; Ingole, Nayana; Wanjare, SW; Nataraj, Gita

doi:10.4103/0255-0857.71807

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…In other fields, the HMA is also a reliable method for identifying mutations, insertions and deletions in genes of interest. Indeed, this assay has been successfully used in epidemiological assays and in searches for linkage in infections, as well as in studies of polymorphism, genotyping, HIV-1 subtyping, phylogenetic analysis, natural HIV-1 recombination, viral genetic heterogeneity and diversity (Heyndrickx et al , 2000; White et al , 2000; Agwale et al , 2001; Mehta et al , 2010; Huang et al , 2011; Mendonça et al , 2011; Manigart et al , 2012). …”

Section: Discussionmentioning

confidence: 99%

Use of the heteroduplex mobility assay and cell sorting to select genome sequences of the CCR5 gene in HEK 293T cells edited by transcription activator-like effector nucleases

et al. 2014

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Engineered nucleases such as zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN) are one of the most promising tools for modifying genomes. These site-specific enzymes cause double-strand breaks that allow gene disruption or gene insertion, thereby facilitating genetic manipulation. The major problem associated with this approach is the labor-intensive procedures required to screen and confirm the cellular modification by nucleases. In this work, we produced a TALEN that targets the human CCR5 gene and developed a heteroduplex mobility assay for HEK 293T cells to select positive colonies for sequencing. This approach provides a useful tool for the quick detection and easy assessment of nuclease activity.

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Section: Discussionmentioning

confidence: 99%

Use of the heteroduplex mobility assay and cell sorting to select genome sequences of the CCR5 gene in HEK 293T cells edited by transcription activator-like effector nucleases

et al. 2014

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“…HIV-1-discordant couples are increasingly viewed as a valuable source of participants for HIV vaccine and prevention trials [124]. Curiously HIV-1 subtype C has been found to be the predominant subtype in sero-discordant couples followed by subtypes B and A, respectively [125].…”

Section: Hiv Diversity Transmission and Diseasementioning

confidence: 99%

HIV Diversity and Classification, Role in Transmission

Duri¹,

Stray‐Pedersen²,

Müller³

2013

AID

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The hallmark of HIV-1 is its extensive genetic diversity that emanates mainly from high mutations. Phylogenetically, HIV can be classified into geographically confined groups, types, subtypes and circulating recombinant forms (CRFs) that are however subject to change over time. HIV genetic diversity may partially explain the observed heterogeneity in HIV prevalence and has also been reported to impact on viral transmissibility and differential rates of disease progression. The aim of this review is to present a simple overview of the principles and concepts of HIV diversity and classification. Tracking the presence of new HIV strains is not only important for surveillance purposes but is also critical in facilitating personalized targeted therapy as well as forming the basis for development of the much anticipated effective vaccines against this scourge.

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Study of HIV-1 subtypes in serodiscordant couples attending an integrated counselling and testing centre in Mumbai using heteroduplex mobility analysis and DNA sequencing

Abstract: HIV-1 subtype C was found to be the predominant subtype of HIV-1 in serodiscordant couples attending our ICTC, followed by HIV-1 subtype B and HIV-1 subtype A, respectively. DNA sequencing was found to be the most reliable method for determining the subtypes of HIV-1.

Cited by 2 publications

References 17 publications

Use of the heteroduplex mobility assay and cell sorting to select genome sequences of the CCR5 gene in HEK 293T cells edited by transcription activator-like effector nucleases

Use of the heteroduplex mobility assay and cell sorting to select genome sequences of the CCR5 gene in HEK 293T cells edited by transcription activator-like effector nucleases

HIV Diversity and Classification, Role in Transmission

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