Study of infection process of five species of Colletotrichum comparing symptoms of glomerella leaf spot and bitter rot in two apple cultivars

Moreira, Rafaele Regina; Zielinski, Erica Camila; Castellar, Camilla; Filho, Armando Bergamin; Mio, Louise Larissa May De

doi:10.1007/s10658-020-02138-y

Cited by 20 publications

(19 citation statements)

References 44 publications

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“…Nevertheless, apple production is diminished via many kinds of fungal diseases (He et al 2018). Apple anthracnose, caused by Colletotrichum spp., causes signi cant yield losses to the growers (Moreira et al 2021). Therefore, analysis of the apple response mechanism is of great signi cance for increasing apple yield.…”

Section: Discussionmentioning

confidence: 99%

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Overexpression of MdIPT8 Encodes an Isopentenyl Transferase Enzyme Enhances Resistance to Colletotrichum Gloeosporioides in apple (Malus Domestica)

Shi

Zhang

Jiang

et al. 2021

Preprint

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Apple (Malus domestica) is a delicious fruit and have high economic value. However, numerous destructive fungi affect apple tree and limit the development of apple production. Many researches showed that salicylic acid and jasmonic acid improved plant resistance to fungal disease, but the potential mechanisms between cytokinin and fungal disease were hardly reported. The IPT gene family encodes the proteins of synthetizing cytokinin and plays an indispensable role in plant biological stress. In our previous study, we found the disease resistance in autotetraploid apple was much better than ‘Hanfu’ and we analyze the gene expression in ‘Hanfu’ and autotetraploid apple. We found the MdIPT8 gene was significantly different expressed. Therefore, we focused on MdIPT genes. We uncovered ten IPT genes in the ‘Hanfu’ whole genome, that are unevenly distributed across five chromosomes. Phylogenetic analysis indicated this family falling into two groups. Moreover, the transcriptional analysis of ten IPT genes indicated MdIPT8 can be induced under Colletotrichum gloeosporioides stress stimulating in apple leaves. The MdIPT8 protein coalesced to green fluorescent protein pinpointed to the chloroplast. Finally, we confirmed that the overexpression of MdIPT8 increased resistance to C. gloeosporioides. Additionally, the endogenous cytokinin content of ‘Hanfu’ leaves increased under the stress of C. gloeosporioides and exogenous cytokinin improved the disease resistance of leaves. Our findings supply an overview of the identification of apple IPT gene family and increases the understanding of cytokinin in apple anthracnose resistance study.

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Section: Discussionmentioning

confidence: 99%

“…When fungi attack apple trees, pathogens seriously hinder their growing and developing (He et al 2018). C. gloeosporioides is a fungal pathogen that constitutes a serious threat to orchards occurring in tropical and subtropical (Moreira et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of MdIPT8 Encodes an Isopentenyl Transferase Enzyme Enhances Resistance to Colletotrichum Gloeosporioides in apple (Malus Domestica)

Shi

Zhang

Jiang

et al. 2021

Preprint

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“…El ciclo de vida de las especies de Colletotrichum incluye etapas reproductivas tanto sexual (teleomorfo) como asexual (anamorfo), la cuales pueden ocurrir en la planta huésped o en los restos de la planta (Da Silva et al, 2020). El tejido vegetal infectado desarrolla acérvulos, que produce masas de conidios en conidióforos (Moreira et al, 2021). Para invadir las células del huésped las esporas del hongo germinan, forman un germen corto y un apresorio que incluye una capa de melanina en la pared celular del apresorio (Fukada et al, 2019).…”

Section: Biología De Colletotrichum Sppunclassified

“…La enfermedad se presenta en el campo cuando coinciden varios factores, entre estos se destacan, la susceptibilidad de la planta y el aumento de la población del patógeno, la humedad relativa, las precipitaciones, temperaturas, vientos fuertes; así como la alta intensidad de luz (Campo, 2011). La temperatura óptima para el crecimiento y la esporulación del patógeno varían entre 26°C y 32°C, con una humedad relativa del 100% (Moreira et al, 2021).…”

Section: Epidemiología De La Antracnosisunclassified

La antracnosis del ñame y estrategias de manejo: una revisión

Arana

Barroso

2020

Temas Agrarios

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El ñame (Dioscorea spp.) es un tubérculo comestible de importancia económica en regiones tropicales, siendo un alimento básico de más de 155 millones de personas. Sin embargo, el rendimiento del cultivo se puede ver afectado por enfermedades devastadoras como la antracnosis (Colletotrichum spp.), la cual puede causar pérdidas de rendimientos hasta un 80% en zonas endémicas, si no se aplican medidas eficaces de manejo. En este sentido, se presenta el siguiente artículo de revisión, el cual tiene como objetivo buscar literatura que permita conocer las investigaciones que se están realizando sobre la antracnosis del ñame, su biología y epidemiología, la sintomatología, etiología y las diferentes estrategias para su manejo. Se describen los síntomas de la enfermedad, la biología y la epidemiología de su principal agente causal Colletotrichum gloeosporioides. Finalmente se presenta las diferentes estrategias de manejo empleadas como son el control genético, cultural, biológico y químico. Se concluye que en el manejo de la enfermedad debe emplearse el método integrado, partiendo de la selección de campos con baja cantidad de inóculo primario, siembra de genotipos resistentes o tolerantes, empleando semilla asexual sana protegida con fungicidas.

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“…GLS is an epidemic disease leading to early defoliation and small sunken lesions (1 to 3mm) that rarely develop into a rot on fruit [1,4]. Apple cultivars descending from the Golden Delicious group, especially the cultivar Gala, are susceptible to GLS, and the disease may cause more than 80% defoliation and diseased fruit before harvest, reducing productivity in subsequent seasons [1,5]. Nevertheless, apple cultivars from Red Delicious, such as the cultivar Fuji, are highly resistant to GLS [6].…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis reveals significant differences in gene expression between pathogens of apple Glomerella leaf spot and apple bitter rot

Jiang

Cai

Yang

et al. 2021

Preprint

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Background: Apple Glomerella leaf spot (GLS) and apple bitter rot (ABR) are two devastating foliar and fruit diseases on apple. The different symptoms of GLS and ABR could be related to different transcriptome patterns. Thus, the objectives of this study were to compare the transcriptome profiles of Colletotrichum gloeosporioides, the common pathogen of GLS and ABR, and to evaluate the genes involvement on pathogenicity.Results: A relatively large difference was discovered between the GLS- and ABR-isolate, and quite a number of differential expression genes associated with pathogenicity were revealed. The DEGs between the GLS- and ABR-isolate were significantly enriched in GO terms of secondary metabolites, however the categories of degradation of various cell wall components did not. A number of genes associate with secondary metabolism were revealed. A total of 17 Cytochrome P450s (CYP), 11 of which were up-regulated while six were down-regulated, and five up-regulated methyltransferase genes were discovered. The genes associated with secretion of extracellular enzymes and melanin accumulation were up-regulated. Four genes associated with degradation of host cell wall, three genes involved in degradation of cellulose, and one gene involved in degradation of xylan were revealed and all up-regulated. In addition, genes involved in melanin synthesis, such as tyrosinase and glucosyltransferase, were highly up-regulated.Conclusions: The penetration ability, pathogenicity of GLS-isolate was greater than that ABR-isolate, which might be indicate that GLS-isolate originated from ABR-isolates by mutation. These results contributed to highlight the importance to investigate such DEGs between GLS- and ABR-isolate in depth.

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Study of infection process of five species of Colletotrichum comparing symptoms of glomerella leaf spot and bitter rot in two apple cultivars

Cited by 20 publications

References 44 publications

Overexpression of MdIPT8 Encodes an Isopentenyl Transferase Enzyme Enhances Resistance to Colletotrichum Gloeosporioides in apple (Malus Domestica)

Overexpression of MdIPT8 Encodes an Isopentenyl Transferase Enzyme Enhances Resistance to Colletotrichum Gloeosporioides in apple (Malus Domestica)

La antracnosis del ñame y estrategias de manejo: una revisión

Comparative transcriptome analysis reveals significant differences in gene expression between pathogens of apple Glomerella leaf spot and apple bitter rot

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