Study of interactions of anthraquinones with DNA using ethidium bromide as a fluorescence probe

Interactions of an anticancer drug lomustine with single and double stranded DNA at physiological conditions analyzed by electrochemical and spectroscopic methods

“…Drug-DNA interactions have been studied by different techniques such as luminescence [3], UV-visible spectroscopy [4][5][6][7][8], fluorescence spectroscopy [9,10], fourier transform infrared spectroscopy (FTIR) [11,12], nuclear magnetic resonance (NMR) [13] and electrochemical methods [14][15][16][17][18][19]. Electrochemical methods have witnessed wide applications that can be applied not only for fundamental studies but also in the practical applications due to their high selectivity, rapidness and low cost instrumentation [20].…”

“…The changes observed here are characteristic of intercalation. Like EB, if LMT intercalates into the helix of dsDNA it would compete with EB for its intercalation sites in dsDNA and the displacement of EB from the dsDNA-EB complex leads to a significant decrease in the fluorescence intensity of the dsDNA-EB complex [7,9,10,17]. It can be inferred from fluorescence studies that the LMT can intercalate through dsDNA efficiently, thus distorting dsDNA structure and inhibiting its replication ability at blood pH (7.4) as well as at stomach pH (4.7).…”

Interaction of Anthracycline 3′-azido-epirubicin with Calf Thymus DNA via Spectral and Molecular Modeling Techniques

“…The negative value of ΔH shows that AEPI binding to ctDNA is exothermic, which is consistent with the negative correlation between binding constants and temperatures. Generally, the negative ΔH and the positive ΔS signify electrostatic force dominates the reaction, the negative ΔH and ΔS imply hydrogen bonding and van der Waals force play the main role, and the positive ΔH and ΔS point out hydrophobic interaction as the major interaction force [18,23]. Consequently, we conclude that the interaction of AEPI binding to ctDNA was mainly driven by hydrogen bonding and van der Waals force.…”

Neuroprotective Effect of EDR Peptide in Mouse Model of Huntington's Disease

“…Luminescence was measured with a Hitachi-850 fluorescence spectrometer using 1 cm-thick quartz cuvette. Luminescence excitation and emission spectra were corrected for the spectral sensitivity of the instrument [34]. Relative viscosity of solutions ηr=η/η0 (where η is the solution viscosity, and η0 is the solvent viscosity) was measured with the Zimm-Crotherstype low-gradient rotation viscometer at flow gradients g = (0.1 ÷ 1) s -1 at 21°C.…”