Study of Local Fields of Dendrite Nanostructures in Hot Spots Formed on SERS-Active Substrates Produced via Template-Assisted Synthesis

Kozhina, E. P.; Andreev, S. N.; Тараканов, В. П.; Bedin, S. A.; Долуденко, И. М.; Naumov, Andrei V.

doi:10.3103/s1062873820120205

Cited by 24 publications

(13 citation statements)

References 18 publications

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“…Although flexible SERS-active substrates possess many inherent advantages, the local electromagnetic field enhancement of SERS substrates is still strongly dependent on their geometric structure [ 18 , 19 ]. Among them, some engineering “hot spots” are excited on the ordered micro-nano structure arrays, not only having sensitive SERS signals but also “hot spots” distributed uniformly [ 5 , 20 , 21 , 22 ]. For example, Kozhina et al used a cost-effective template-assisted electrodeposition method to grow metal nanowires (NWs) in polymer trace etching film (TM) holes to prepare SERS substrates [ 21 , 22 ].…”

Section: Introductionmentioning

confidence: 99%

LSPR Tunable Ag@PDMS SERS Substrate for High Sensitivity and Uniformity Detection of Dye Molecules

et al. 2022

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At present, the use of efficient and cost-effective methods to construct plasmonic surface-enhanced Raman scattering (SERS) substrates of high sensitivity, uniformity and reproducibility is still crucial to satisfy the practical application of SERS technology. In this paper, a localized surface plasmonic resonance (LSPR) tunable flexible Ag@PDMS substrate was successfully constructed by the low-cost bio-template-stripping method and magnetron sputtering technology. The theory proves that the local electromagnetic field enhancement and “hot spot” distribution is adjustable by modifying the size of the optical cavity unit in the periodicity nanocavity array structure. Experimentally, using rhodamine 6G (R6G) as the target analyte, the SERS performance of optimal Ag@PDMS substrate (Ag film thickness for 315 nm) was researched in detail, which the minimum detection limit was 10−11 M and the enhancement factor was calculated as 8.03 × 108, indicating its high sensitivity. The relative standard deviation (RSD) was calculated as 10.38%, showing that the prepared substrate had excellent electromagnetic field enhancement uniformity. At last, the trace detection of Crystal violet (CV, LOD = 10−9 M) and the simultaneous detection of three common dyes (R6G, CV and Methylene blue (MB) mixture) were also realized. This result suggests that the SERS substrate has a good application prospect in the quantitative and qualitative detection of dye molecules.

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Section: Introductionmentioning

confidence: 99%

LSPR Tunable Ag@PDMS SERS Substrate for High Sensitivity and Uniformity Detection of Dye Molecules

et al. 2022

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“…The application of iodide-containing electrolyte on tracking membranes (TMs) enables to synthesize dendritic nanostructures on the vertices of silver NWs. Furthermore, they have modelled the electromagnetic field near the patterned nanostructures upon application of visible laser radiation and reported considerable electric filed enhancement [32]. Kozhina et al, also developed a simple method for patterning metal nanowires (NWs) by utilizing polymer track-etched membranes (TM) as template for electrodeposition.…”

Section: Introductionmentioning

confidence: 99%

An Electrochemical and Raman Scattering Dual Detection Biosensor for Rapid Screening and Biomolecular Profiling of Cancer Biomarkers

et al. 2022

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Detecting circulating biomarkers sensitively and quantitatively is paramount for cancer screening, diagnosis, and treatment selection. Particularly, screening of a panel of circulating protein biomarkers followed by mapping of individual biomarkers could assist better diagnosis and understanding of the cancer progression mechanisms. Herein, we present a miniaturized biosensing platform with dual readout schemes (electrochemical and Surface enhanced Raman scattering (SERS)) for rapid cancer screening and specific biomarker expressional profiling to support cancer management. Our approach utilizes a controlled nanomixing phenomena under alternative current electrohydrodynamic condition to improve the isolation of cancer-associated circulating proteins (i.e., Epidermal growth factor receptor (EGFR), BRAF, Programmed death-ligand 1 (PD-L1)) with antibody functionalized sensor surface for rapid and efficient isolation of the targets and subsequent labelling with SERS nanotags. The method employs Differential Pulse Voltammetry (DPV) for rapidly screening for the presence of the circulating proteins on biosensor surface irrespective of their type. Upon positive DPV detection, SERS is applied for sensitive read-out of individual biomarkers biomarker levels. In a proof-of-concept study, we demonstrate the dual detection biosensor for analysing circulating BRAF, EGFR and PDL-1 proteins and successfully screened both ensemble and individual biomarker expressional levels as low as 10 pg (1 ng/mL). Our findings clearly indicate the potential of the proposed method for cancer biomarker analysis which may drive the translation of this dual sensing concept in clinical settings.

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“…As an example, substrates with arrays of nanowires (NWs) [18][19][20] provide SERS EF up to 10 7 . Also, a higher aspect ratio (length to diameter) demonstrates a bigger enhancement factor [21][22][23]. Moreover, SERS substrates with the NWs array may demonstrate bigger EF due to the NWs leaning feature that is caused by capillary force.…”

Section: Introductionmentioning

confidence: 99%

Ag-Nanowire Bundles with Gap Hot Spots Synthesized in Track-Etched Membranes as Effective SERS-Substrates

et al. 2021

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This paper presents a cost-effective approach for the template-assisted electrodeposition fabrication of substrates for surface-enhanced Raman scattering (SERS) with metal nanowires (NWs) grown in pores of polymer track-etched membranes (TM). This technique allows the synthesis of NWs array with its certain surface density and diameter (from dozen to hundreds of nm). NWs length also may be varied (order of μm) by controlling deposition time. Here we grow vertical Ag-NWs which are leaning towards their nearest neighbors, forming self-assembled bundles whose parameters depend on the NW aspect ratio (length to diameter). We show that in such bundles there are “hot spots” in the nm-gaps between NWs tips. Computer simulations have demonstrated a strong enhancement of the electric field within these hot spots; thus, the Raman signal is markedly amplified for analyte molecules placed directly inside the gaps. We have experimentally proved the potential of this SERS-technique on the example of 4-Mercaptophenylboronic acid (4-MPBA). For 4-MPBA the maximal enhancement of Raman signal was found at NWs length of ~1.6 μm and diameter of ~100 nm. The effect is higher (up to twice) if “wet” substrate is used just immediately after the TM polymer removal so that the tips are brought to lean after analyte exposure. We suggest this new type of nanostructured SERS-substrates as a base of effective sensing of extremely low concentration of analytes.

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Study of Local Fields of Dendrite Nanostructures in Hot Spots Formed on SERS-Active Substrates Produced via Template-Assisted Synthesis

Cited by 24 publications

References 18 publications

LSPR Tunable Ag@PDMS SERS Substrate for High Sensitivity and Uniformity Detection of Dye Molecules

LSPR Tunable Ag@PDMS SERS Substrate for High Sensitivity and Uniformity Detection of Dye Molecules

An Electrochemical and Raman Scattering Dual Detection Biosensor for Rapid Screening and Biomolecular Profiling of Cancer Biomarkers

Ag-Nanowire Bundles with Gap Hot Spots Synthesized in Track-Etched Membranes as Effective SERS-Substrates

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