Study of mcr-1 Gene-Mediated Colistin-Resistance in Gram-Negative Isolates in Egypt

Emara, Manar M.; Abd-Elmonsef, Marwa M. E.; Elnasr, Lobna Abo; Elfeky, Aziza

doi:10.21608/ejmm.2019.282890

Cited by 7 publications

(5 citation statements)

References 21 publications

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“…These data revealed high colistin resistance percentage compared to previous studies that showed complete sensitivity to colistin 10 and another study that demonstrated that only 1 isolate from 66 P. aeruginosa isolates was resistant to colistin. 28 In the present study, the homology analysis (using ERIC-PCR) revealed that plasmidmediated carbapenemases and colistin resistance genes could be easily transmitted across the P. aeruginosa isolates. Most of the tested isolates were unrelated to each other; however, they all included one or more carbapenemase gene.…”

mentioning

confidence: 55%

“…Screening for the existence of β-lactamases genes involving bla VIM , bla IMP , bla NDM-1 , bla KPC and bla OXA-48 and colistin resistance gene (mcr-1) was established by PCR using the primer sets listed in Table 1. [28][29][30][31][32] The PCR reactions were established in the volume of 25 μL. Each PCR reaction consisted of 3 μL of template DNA, 1 μL forward primer (10 μM), 1 μL of reverse primer (10 μM), 12.5 μL Dream Taq PCR master mix 2x (Fermentas, USA) and 7.5 μL nuclease free water.…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

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Emergence of Multidrug-resistant Carbapenemases and MCR-1 Producing Pseudomonas aeruginosa in Egypt

Aboelsuod¹,

Sonbol²,

El-Banna³

et al. 2023

J. Pure Appl. Microbiol.

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Pseudomonas aeruginosa is an expedient Gram-negative bacterium, which is characterized by its ability to acquire antimicrobial resistance. In this study, 56 unrepeatable carbapenem-resistant P. aeruginosa isolates were gathered from various clinical sources from hospitals in Cairo and Mansoura universities. The isolates exhibited diminished susceptibility towards carbapenems, quinolones, aminoglycosides and chloramphenicol by using disc diffusion method. Carbapenemase production was confirmed among the isolates, where all the 56 P. aeruginosa isolates harboured carbapenemase genes including blaVIM (43 isolates), blaKPC (38 isolates), blaNDM-1 (17 isolates), blaIMP (16 isolates) and blaOXA-48 (15 isolates). Among the isolates, 13 carried only one carbapenemase gene, while 43 isolates carried multiple carbapenemase genes. MCR-1 production was confirmed in 10 of the tested isolates by detecting the mcr-1 gene encoding for the colistin resistance. Enterobacterial repetitive intergenic consensus-PCR (ERIC-PCR) evaluation showed that the tested isolates were unrelated to each other. Therefore, this study rises the danger of emergence of MDR P. aeruginosa resistant to carbapenems coupled with other antimicrobials including colistin, which is regarded as the last reservoir for the management of infections caused by MDR Gram-negative pathogens. Early inspection of resistance patterns in MDR organisms is an important tool to control and prevent infections via limiting the spread of these pathogens.

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confidence: 55%

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Emergence of Multidrug-resistant Carbapenemases and MCR-1 Producing Pseudomonas aeruginosa in Egypt

Aboelsuod¹,

Sonbol²,

El-Banna³

et al. 2023

J. Pure Appl. Microbiol.

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“…Zafer et al reported 22 (4.9%) colistin-resistant K. pneumoniae isolated over 18 months from cancer patients in Egypt [44] . Colistin resistance was detected in 8 (7.5%) K. pneumoniae in Tanta University Hospitals according to Ezzat et al [45] . Rabie et al found that 17.2% of K. pneumoniae isolates were resistant to colistin at Zagazig University Hospitals [46] .…”

Section: Discussionmentioning

confidence: 99%

Prevalence of the colistin resistance gene MCR-1 in colistin-resistant Klebsiella pneumoniae in Egypt

Abozahra¹,

Gab-Allah²,

Abdelhamid

2023

AIMSMICRO

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<abstract> <italic>Klebsiella pneumoniae</italic> is a nosocomial pathogen with high morbidity and mortality rates in hospitalized patients. The emergence of multidrug-resistant <italic>K. pneumoniae</italic> has become more challenging to treat, with the prevalence of colistin-resistance. Therefore, reliable methods for detecting colistin resistance are required. Many plants' essential oils have antimicrobial activity and have been used to combat multiple antibiotic resistances. This study aimed to investigate the characterization and prevalence of the colistin resistance gene <italic>mcr-1</italic> in <italic>K. pneumoniae</italic> in Egypt, evaluate rapid polymyxin NP test, determine the transferability of <italic>mcr-1</italic> gene, and study the synergistic activity of eugenol combined with colistin against <italic>K. pneumoniae</italic> isolates. Eighty-two <italic>K. pneumonia</italic> isolates were collected from different human samples, followed by antibiotic susceptibility testing, rapid polymyxin NP test, and detection of the <italic>mcr-1</italic> gene and its transfer frequency. Determination of the MICs of colistin alone and in combination with eugenol was performed, then <italic>mcr-1</italic> gene expression was determined in the presence of eugenol. Thirty-two isolates (39%) were colistin-resistant. Rapid polymyxin NP test failed to detect resistant isolates with MICs below 32 µg/mL. Detection of <italic>mcr-1</italic> gene was made in 27 (84%) of colistin resistant isolates. The rest isolates possess alteration in the <italic>mgrB</italic> gene which probably causes colistin resistance. The <italic>mcr-1</italic> gene was transferred by conjugation to other sensitive isolates. MIC of eugenol ranged from 416 to 1664 µg/mL, and FICI ranged from 0.265 to 0.75. Results also revealed suppression of <italic>mcr-1</italic> gene expression in the presence of sub MIC of eugenol. Our results demonstrated a high prevalence of <italic>mcr-1</italic> in Egypt and its ability to transfer to other strains. Difficult determination of colistin-resistant isolates with low values with rapid polymyxin NP test was apparent. Eugenol exerted a synergistic effect with colistin and improved its antimicrobial activity. </abstract>

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“…This is because E. coli isolates that produce MCR -1 have been determined in animal food products [9]. The thoughtless use of colistin in the veterinary sector, particularly in the lack of severe lawmaking has contributed to the global dispersal of the MCR -1 gene in 10% of animal isolates and in 0.1–2% of human isolates [10].…”

Section: Introductionmentioning

confidence: 99%

Colistin resistance genes inEscherichia coliisolated from patients with urinary tract infections

Al Momani,

Ata,

Maslat

2024

Preprint

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Introduction: Antimicrobial resistance is alarmingly high because it happens in humans, environment, and animal sectors from a "One Health" viewpoint. Due to the fact, that Escherichia coli (E. coli) is broadly disseminated in all sectors, the food web and the environment may have a role in carrying colistin resistance genes from animals to humans. The rise of plasmid-mediated mobile colistin resistance (MCR) genes threatens colistin efficacy, which is the last line to remedy Gram-negative infections multidrug resistance (MDR). Objectives: The current study aimed to investigate development of colistin resistance (MCR 1-5) genes between E. coli isolated from patients with urinary tract infections (UTI) in Jordan. Methods: E. coli (n=132) isolated were collected from urine specimens. E. coli isolated from human UTI patients were examined for genes resistance to colistin MCR (1-5). All isolates were investigated against 20 antimicrobials utilizing the standard disk diffusion method. For analysis of colistin resistance, the broth microdilution technique was employed. In addition, the MCR (1-5) genes were detected by multiplex PCR assay. Results: Out of 132 isolates, one isolate was colistin-resistant, having a minimum inhibitory concentration of 8 μg/mL and possessing the MCR -1 gene. A total of 132 E. coli isolates showed high resistance against penicillin, amoxicillin, cephalexin, nalidixic acid, tetracycline, and cefepime in the percentages of 100%, 79.55%, 75.76%, 62.88%, 58.33%, and 53.79%, respectively. However, resistance was lowest towards fosfomycin (6.06%), florfenicol (10.61%), and chloramphenicol (15.91%). Significant differences were observed between E. coli isolated from pediatrics and those isolated from adults. Conclusion: This is the first report on the presence of the plasmid-coded MCR-1 gene recovered from E. coli from a patient with UTIs in Jordan. That is threatening as colistin is the last line used for infections induced by MDR gram-negative bacteria. There is a crucial need for control and harsh utilization of antibiotics to control and prevent the emergence and prevalence of colistin resistance genes.

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Study of mcr-1 Gene-Mediated Colistin-Resistance in Gram-Negative Isolates in Egypt

Cited by 7 publications

References 21 publications

Emergence of Multidrug-resistant Carbapenemases and MCR-1 Producing Pseudomonas aeruginosa in Egypt

Emergence of Multidrug-resistant Carbapenemases and MCR-1 Producing Pseudomonas aeruginosa in Egypt

Prevalence of the colistin resistance gene <i>MCR-1</i> in colistin-resistant <i>Klebsiella pneumoniae</i> in Egypt

Colistin resistance genes inEscherichia coliisolated from patients with urinary tract infections

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