Study of Metabolic Biomarkers in Plasma of Patients with Dyslipidemia Based on Gas Chromatography Mass Spectrometry and Graphical Models

Bai, Dong Hoon; Jian-nan, Song

doi:10.3724/sp.j.1096.2012.20012

Cited by 3 publications

(3 citation statements)

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“… 26 Other notable positive associations were observed for glutamate, which has previously been linked to insulin resistance 27 and glycerol, which is perturbed in conditions of dyslipidaemia. 28 The reduction is oestrogen levels post menopause and the associated physiological changes likely drive the differences in metabolite associations by menopausal status. A recent study suggested that menopause attenuates metabolism, particularly lipids and inflammatory biomarkers, independently of advancing age.…”

Section: Discussionmentioning

confidence: 99%

Investigation of circulating metabolites associated with breast cancer risk by untargeted metabolomics: a case–control study nested within the French E3N cohort

et al. 2021

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Background Perturbations in circulating metabolites prior to a breast cancer diagnosis are not well characterised. We aimed to gain more detailed knowledge to help understand and prevent the disease. Methods Baseline plasma samples from 791 breast cancer cases and 791 matched controls from the E3N (EPIC-France) cohort were profiled by nuclear magnetic resonance (NMR)-based untargeted metabolomics. Partial least-squares discriminant analysis (PLS-DA) models were built from NMR profiles to predict disease outcome, and odds ratios and false discovery rate (FDR)-adjusted CIs were calculated for 43 identified metabolites by conditional logistic regression. Results Breast cancer onset was predicted in the premenopausal subgroup with modest accuracy (AUC 0.61, 95% CI: 0.49–0.73), and 10 metabolites associated with risk, particularly histidine (OR = 1.70 per SD increase, FDR-adjusted CI 1.19–2.41), N-acetyl glycoproteins (OR = 1.53, FDR-adjusted CI 1.18–1.97), glycerol (OR = 1.55, FDR-adjusted CI 1.11–2.18) and ethanol (OR = 1.44, FDR-adjusted CI 1.05–1.97). No predictive capacity or significant metabolites were found overall or for postmenopausal women. Conclusions Perturbed metabolism compared to controls was observed in premenopausal but not postmenopausal cases. Histidine and NAC have known involvement in inflammatory pathways, and the robust association of ethanol with risk suggests the involvement of alcohol intake.

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Section: Discussionmentioning

confidence: 99%

Investigation of circulating metabolites associated with breast cancer risk by untargeted metabolomics: a case–control study nested within the French E3N cohort

et al. 2021

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“…Sample preparation followed our previous method. , A 99:1 volume ratio of BSTFA and TMCS was used as the derivatization reagent. GC–MS analysis was operated on a QP2010 GC–MS (Shimadzu, Japan) with a RTX-5 GC chromatographic column (30 m × 0.32 mm × 0.25 μm, Agilent J&W, U.S.A.).…”

Section: Materials and Methodsmentioning

confidence: 99%

Ganoderic Acid A To Alleviate Neuroinflammation of Alzheimer’s Disease in Mice by Regulating the Imbalance of the Th17/Tregs Axis

Wang

Yang

et al. 2021

J. Agric. Food Chem.

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Ganoderic acid A (GAA) is a kind of lanostane-type triterpenoid isolated from Ganoderma lucidum. Imbalance of the Th17/Tregs axis exists in the progress of neuroinflammation of Alzheimer's disease (AD). In this study, the alleviating neuroinflammatory effect of GAA on D-galactose mice was studied from the aspect of regulating the imbalance of the Th17/Tregs axis. The Morris water maze test was used to evaluate the cognitive ability of AD mice. Flow cytometry was used to detect the percentages of IL-17A, IL-17F, IL-21, IL-22, and CD4 + CD25 + Foxp3 + in peripheral blood. Transmission electron microscopy was used to assess the cerebral mitochondrial ultrastructure. Metabolomic analysis based on gas chromatography−mass spectrometry was used to evaluate the mitochondrial dysfunction metabolism. Western blot analysis was used to detect the protein expressions of cytokines secreted by Th17 cells and Treg cells in the brain. As the results show, GAA has an alleviating neuroinflammatory effect on AD mice via regulating the imbalance of the Th17/Tregs axis. The potential mechanism was related to inhibition of the JAK/STAT signaling pathway induced by Th17 cells and enhancement of the mitochondrial oxidative phosphorylation by regulating Treg cells, thereby improving mitochondrial dysfunction of AD mice.

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“…Referred to the previous method to process the sample (Bai & Song, 2012; Yan Zhang et al, 2018). Methanol (250 μl) was added to 100 μl urine (added with 60 IU/100 μl urease beforehand), liver homogenate, and serum.…”

Section: Methodsmentioning

confidence: 99%

Gentiopicroside prevents alcoholic liver damage by improving mitochondrial dysfunction in the rat model

Yang

Wang

Song

et al. 2020

Phytotherapy Research

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Gentianae Radix et Rhizoma is a medical plant that is widely cultivated in China, North Korea, Japan, and Russia, and gentiopicroside is one of its major active compounds. In this study, the hepatoprotective activity of gentiopicroside on rats with alcoholic liver damage (ALD) was evaluated using the transaminase and blood lipid levels and antioxidant capacity. The potential mechanism of hepatoprotective effect of gentiopicroside was evaluated by mitochondrial function detection, gas chromatography–mass spectrometry (GC–MS) metabolomic analysis, and anti‐apoptosis analysis. Results showed that the gentiopicroside exhibited good hepatoprotective activity on rats with ALD by decreasing the transaminase levels, regulating the blood lipid levels, and increasing the antioxidant capacity. The potential mechanisms were related to regulating mitochondrial dysfunction by recovering mitochondrial membrane potential level, adenosine triphosphate concentration, activities of key enzymes in tricarboxylic acid cycle, and activities of complex I‐V, regulating micromolecular metabolism and anti‐apoptosis. These findings supported the further exploration of Gentianae Radix et Rhizoma as effective phytotherapy to prevent and treat ALD.

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Study of Metabolic Biomarkers in Plasma of Patients with Dyslipidemia Based on Gas Chromatography Mass Spectrometry and Graphical Models

Cited by 3 publications

References 0 publications

Investigation of circulating metabolites associated with breast cancer risk by untargeted metabolomics: a case–control study nested within the French E3N cohort

Investigation of circulating metabolites associated with breast cancer risk by untargeted metabolomics: a case–control study nested within the French E3N cohort

Ganoderic Acid A To Alleviate Neuroinflammation of Alzheimer’s Disease in Mice by Regulating the Imbalance of the Th17/Tregs Axis

Gentiopicroside prevents alcoholic liver damage by improving mitochondrial dysfunction in the rat model

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