Study of Metabolic Profiling Changes in Colorectal Cancer Tissues Using 1D¹H HR-MAS NMR Spectroscopy

Abstract

Metabolomics is a field that studies systematic dynamics and secretion of metabolites from cells to understand biological pathways based on metabolite changes. The metabolic profiling of intact human colorectal tissues was performed using high-resolution magic angle spinning (HR-MAS) NMR spectroscopy, which was unnecessary to extract metabolites from tissues. We used two different groups of samples, which were defined as normal and cancer, from 9 patients with colorectal cancer and investigated the samples in NMR experiments with a water suppression pulse sequence. We applied target profiling and multivariative statistical analysis to the analyzed 1D NMR spectra to identify the metabolites and discriminate between normal and cancer tissues. Cancer tissue showed higher levels of arginine, betaine, glutamate, lysine, taurine and lower levels of glutamine, hypoxanthine, isoleucine, lactate, methionine, pyruvate, tyrosine relative to normal tissue. In the OPLS-DA (orthogonal partial least square discriminant analysis), the score plot showed good separation between the normal and cancer groups. These results suggest that metabolic profiling of colorectal cancer could provide new biomarkers.

Metabolomics Biomarkers for Breast Cancer

“…This has considerable clinical potential, especially as the HR-MAS NMR analysis is fast, relatively inexpensive and nondestructive. A similar approach is also being pursued for other types of cancer [33,34,35,36,37]. …”

Applications of NMR spectroscopy based metabolomics: a review

“…To extract useful information from complicated NMR data, pattern recognition methods such as multivariate statistical analysis and target profiling The following metabolites were identified using the Chenomx 500 MHz library. 43 identification has been routinely performed for many years in NMR-based metabolomics, it was largely done on compound-selective basis. Additionally, several companies (Agilent Chenomx and Bruker Biospin) have produced commercial products that implement these concepts in convenient, easy-to-use software programs.…”

“…18 samples were classified clearly into two groups. ▲: cancer •: normal 43 products for the anti-bacterial material. However, AgNPs have been reported as toxic to the mammalian cell, lung, liver, brain and other organs.…”

“…43 Fig.3 is HR-MAS 1 H NMR spectrum of colorectal normal tissue which was extracted from the patient by biopsy. The spectrum shows the metabolites which were identified by the comparison with a library.…”

A systems biology approach towards child obesity and obesity-related diseases: integration of clinical, genetic, hormonal and NMR metabonomic factors

“…Recently, full resolution analysis is preferred in contemporary metabonomics analysis (Agnolet et al, 2010, Kim et al, 2013. However, given the statistical weakness of our data at a resolution of 0.04 ppm bucketing (which usually yields statistically relatively robust results when compared to higher resolution bucketing due to the lower number of variables being considered), it is unlikely that our data would provide further discriminatory information at higher resolutions.…”