Study of metabolite variability in Plumbago zeylanica Linn. collected from different localities of the Gangetic plains of India

Shukla, Pushpendra Kumar; Misra, Ankita; Patra, Kamal Kant; Srivastava, Sharad

doi:10.1007/s00764-020-00018-z

Cited by 4 publications

(1 citation statement)

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“…The objective of this work was, hence, to determine a simple, solid, cost effective, and rapid HPTLC method for quantification of embelin in different parts of Embelia ribes, E. tsjeriam-cottam, E. basaal, E. adnata, and E. gardneriana. Marker compounds are being employed for evaluation, standardization, and characterization of medicinal plants [18], and HPTLC is generally accepted applied analytical technique for this [19][20][21]. The developed method was verified as per the International Conference on Harmonization (ICH) guidelines [22].…”

Section: Introductionmentioning

confidence: 99%

Embelin: an HPTLC method for quantitative estimation in five species of genus Embelia Burm. f.

Vijayan

Raghu

2021

Futur J Pharm Sci

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Background The plants belonging to the genus Embelia, a significant tropical genus with many biological activities, are benefiting because of their robust medicinal properties. Embelin is one of the principal bioactive molecules responsible for the medicinal properties of the genus Embelia. The quantification of the embelin compound among different species in this genus has not yet been investigated, so still uncertain which species and which part should be accepted. The present study was intended to establish a speedy and precise high-performance thin-layer chromatographic (HPTLC) method for quantitative study of embelin in various plant parts of Embelia ribes, Embelia tsjeriam-cottam, Embelia basaal, Embelia adnata, and Embelia gardneriana. Result This research confirmed the method as per the International Conference on Harmonization (ICH) guidelines. We achieved separation on silica gel 60 F254 HPTLC plates using propanol: butanol: ammonia (7:3:7 v/v/v) as a mobile phase. Densitometry scanning performed for detection and quantification at 254 nm and 366 nm. Among the species investigated, the highest amount of embelin was found in E. ribes fruits. Conclusion Embelia ribes fruits are the best source of embelin. Embelin was first described in the endemic species, such as E. adnata and E. gardneriana. The method illustrated in this research may be applied for quantification of embelin and fingerprint analysis of other species within Embelia genus or described genera and chemo taxonomic studies of this genus.

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Section: Introductionmentioning

confidence: 99%