Study of Methicillin-resistant Staphylococcus aureus and Species of Coagulase Negative Staphylococci Isolated from Clinical Specimens and Their Antimicrobial Susceptibility Pattern Among Patients Ahwaz, Iran

Abstract

Background: Streptococcus mutans, an organism colonizing the oral cavity, is considered to be the main cause of dental caries. In addition to dental caries and related pyogenic dental infections, S. mutans is also a very important endocarditis agent. The participation of this microorganism in both oral and non-oral diseases has prompted interest in the knowledge of this susceptibility to antimicrobial agents. The aim of this study was to determine the antimicrobial susceptibility of S. mutans biotypes isolated from children dental caries free. Methods: Unstimulated saliva samples were collected from 60 children, diluted and cultured onto mitis salivarius bacitracin (MSB) agar plates for the selective isolation and enumeration of S. mutans. The MSB agar plates were incubated anaerobically (H 2 :CO 2 : N 2 10:10:80) for two days a 37 • C. The biotyping of S. mutans was realized using the api Zym system (bioMérieus; Marcy-lÉtoile, France). The minimal inhibitory concentrations were evaluated against penicillin, amoxycillin, cefazolin, erythromycin, clindamycin, imipenem and vancomycin by using an agar dilution method with concentrations between 0.003 and 32 ug/ml. Results: Twenty biotypes were detected in the 74 strains of S. mutans isolated. The most frequent byotipes were the 2,3,10 and 13 (n = 6). Nineteen out of the 23 children in whom 2 strains of S. mutans were isolated, and 7 children in whom 3 strains of S. mutans were isolated presented different biotypes of S. mutans. All biotypes were highly sensitive to penicillin, amoxicillin, cefazolin, erythromycin, clindamycin, imipenem and vancomycin; 50 and 90% of S. mutans strains were inhibited by using concentrations of less than 0.12 and 0.5 ug/ml, respectively, for all antibiotics studied. Conclusion: S. mutans biotypes were highly sensitive to he antibiotics tested.

Methicillin resistance & inducible clindamycin resistance in Staphylococcus aureus

“…The findings on MRSA in our study was similar to that reported by Mehdinejad et al 8. In a study by Prabhu et al 9, 20 per cent MRSA and six per cent MSSA showed iCR contributing to an overall ten per cent prevalence of iCR.…”

Detection of methicillin resistant Staphylococcus areus isolated from nasal carriage of health care workers by polymerase chain reaction

“…Results of this study are in agreement with the findings of Mahesh et al (2015) those who reported that overall nosocomial infection rates were 42 (41.17%) of the HCWs. In this study, prevalence of MRCoNS rate was lower than other previous reports done in other countries such as France, Germany, Greece, Italy and Turkey (Sader et al, 2007;Mehdinejad et al, 2008). The variations in nasal carriage prevalence of MRCoNS might be due to the variations in geographical distributions of bacterial strains or due to the infection control practices.…”

Prevalence, Antimicrobial Susceptibility Pattern and Risk Factors of Mrsa Isolated From Clinical Specimens Among Military Patients at 48 Medical Compound in Sana'a City-Yemen

“…This result is different from that reported in Asian countries in which the rate of vancomycin resistant was not more than 10% 21 . Also the incidence of VRSA in the Asian countries has been documented by Kaleem et al, 22 in Pakistan to be 3.3%, 6% in India, by Sonavane and Mathur 23 , 7.5% in Iran by Mehdinejad et al, 24 , and 9% in Jordan by AlZoubi et al, 3 .…”