Study of near-infrared light-induced excitation of upconversion nanoparticles as a vector for non-viral DNA delivery

Abstract

Clinical requirements have necessitated the development of biomedical nanomaterials that can be implanted into tissues or bodies.

DNA-functionalized metal or metal-containing nanoparticles for biological applications

“…One strategy is the simple mixing of DNA with ligand-free UCNPs (treated with hydrochloric acid). 65 In this strategy, the negatively charged phosphate backbone of DNA molecules can attach to the surface of the positively charged UCNPs, resulting in strong absorption with an uncontrolled conformation. Despite the strong electrostatic interaction between DNA molecules and UCNPs, their further bioapplications is significantly limited by the following issues: first, the nonspecific interaction can induce a serious aggregation of the UCNPs, and second, the attached DNA cannot hybridize to cDNA or exert normal functions because of the strong interactions between DNA and the UCNP surface.…”

Bioimaging with Upconversion Nanoparticles

“…An ideal nanoparticle‐based bioimaging probe can be modified to target and enable observation of specific intracellular organelles, proteins, DNA strands, or other areas of interest. Briefly, UCNPs have been coated with DNA strands, [ 76 ] antibodies, [ 187 ] amine groups, [ 188 ] cell membranes, [ 73 ] and ligands/polymers/surface charges [ 30,181,189–191 ] among others, and have been engineered to be activatable for imaging or release of loaded molecules (Table 1). [ 180 ] For example, using Nile red dye derivative‐modified UCNPs, iron ions were detected in cells at concentrations of 89.6 nM.…”

“…[ 20 ] Similarly, UCNPs with a mesoporous silica and amine shell were loaded with DNA to examine transfection and DNA delivery to primary rat heart cell lines. [ 188 ]…”

Perovskite Quantum Dots Modulating Upconversion Nanomaterials for Cancer Early Detections

“…The typical high-temperature coprecipitation method was used to prepare UCNPs luminescent nanomaterials [39,40]. The TEM image of UCNPs is shown in Fig.…”