Study of novel chitosan‐gelatin artificial skin in vitro

Abstract

A novel absorbable scaffold composed of chitosan and gelatin was fabricated by freezing and lyophilizing methods, resulting in an asymmetric structure. This bilaminar texture is suitable for preparing a bilayer skin substitute. The methods employed to confirm the applicability of this chitosan-gelatin scaffold as an ideal skin substitute were a water uptake ability test, in vitro fibroblast proliferation, and scaffold tests in which fibroblasts were co-cultured with keratinocytes. The chitosan-gelatin scaffolds were more wettable and adsorbed more water than did chitosan alone. In static cell culture the thinner scaffold is better than the thicker one, and because of diffusion limitations in the scaffold, culture time must be within 3 weeks before transplantation to living tissues. Keratinocytes were co-cultured with fibroblasts in chitosan-gelatin scaffolds to construct an artificial bilayer skin in vitro. The artificial skin obtained was flexible and had good mechanical properties. Moreover, there was no contraction observed in the in vitro cell culture tests. The data from this study suggest that chitosan-gelatin scaffolds are suitable for skin tissue engineering goals.

PLLA–collagen and PLLA–gelatin hybrid scaffolds with funnel-like porous structure for skin tissue engineering

“…Gelatin is the partially denatured product of collagen. The bioactivity of gelatin is lower than that of collagen because of the denaturation [19][20][21]. Therefore, the different proliferation rates can be attributed to the different bioactivity of collagen and gelatin.…”

Preparation and characterization of macroporous chitosan–gelatin/β‐tricalcium phosphate composite scaffolds for bone tissue engineering

“…20 With hopes of increased cytocompatibility and elucidation of a desirable cellular response, a porous chitosan/gelatin network scaffold was developed for cartilage tissue engineering and artificial skin. 27,28 Further, biomimetic 3D HA/chitosan-gelatin network composite scaffolds were prepared for bone tissue engineering. 29 In this article, we report on the manufacture of macroporous chitosan-gelatin/␤-tricalcium phosphate (CS-Gel/␤-TCP) composite scaffolds through a freeze-drying technique.…”

Effect of a Three-Dimensional Chitosan Porous Scaffold on the Differentiation of Mesenchymal Stem Cells into Chondrocytes

“…It is also a promising biomaterial for tissue engineering. Although gelatin alone has poor mechanical stability and is rapidly degraded, when it is cross-linked to chitosan its mechanical stability increases and it becomes a potential scaffold for cartilage tissue engineering [Mao et al, 2003a].…”