Study of osmoadaptation mechanisms of halophilic Halomonas alkaliphila XH26 under salt stress by transcriptome and ectoine analysis

Zhang, Tiantian; Zhang, Xin; Li, Yongzhen; Qiao, Lei; Miao, Zengqiang; Xing, Jiangwa; Zhu, Derui

doi:10.1007/s00792-022-01256-1

Cited by 10 publications

(11 citation statements)

References 67 publications

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“…To confirm the crucial role of ectoine synthesis in osmoadaptation, the intracellular concentrations of compatible solutes were measured in cells grown at four salinities (4%, 8%, 12%, and 16%) for 48 h (Figure 8). The accumulation of ectoine was dependent on both salinity and cell growth phase, which is consistent with previous studies (Hänelt & Müller, 2013;Salvador et al, 2018;Tao et al, 2016;Zhang et al, 2022). There was a positive correlation between the accumulation of ectoine and cell growth in A. halolimnae.…”

Section: Ectoine and Hydroxyectoine Play A Major Role In Salt Adaptationsupporting

confidence: 92%

“…Taken together, the up-regulation of amino acid and carbohydrate metabolism under salt stress provides energy and precursor molecules for osmoprotectant synthesis and uptake in the salt-stressed A. halolimnae BZ-SZ-XJ29 T . This has already been reported in halophilic bacteria (Chen et al, 2021;Kindzierski et al, 2017;Long et al, 2018;Yaakop et al, 2016;Zhang et al, 2022).…”

Section: Effect Of Salt Stress On Cellular Metabolism and Membrane Tr...supporting

confidence: 75%

“…In agreement with proteomic analysis, ectABC were all up-regulated in the three NaCl comparison groups (Table 2, Figure S3). This result differs from that of Halomonas alkaliphila XH26 (Zhang et al, 2022). In H. alkaliphila XH26, the expression levels of lysC, asd, ectA, and ectC genes were significantly higher in 15% NaCl compared to 6% NaCl, with no significant change in the expression of the ectA gene.…”

Section: Ectoine and Hydroxyectoine Play A Major Role In Salt Adaptationcontrasting

confidence: 71%

“…The intercellular concentration of compatible solute and final cell density (OD 600 ) of Alkalicoccus halolimnae cultured at different NaCl concentrations (4%, 8%, 12%, and 16%) for 48 h. Kindzierski et al, 2017;Zhang et al, 2022). The data indicate that the accumulation of ectoine plays a critical role in the adaptation of A. halolimnae to continuous salt exposure.…”

Section: Ectoine and Hydroxyectoine Play A Major Role In Salt Adaptationmentioning

confidence: 95%

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Tandem mass tag‐based quantitative proteomics reveals osmotic adaptation mechanisms in Alkalicoccus halolimnae BZ‐SZ‐XJ29^T, a halophilic bacterium with a broad salinity range for optimal growth

Qing-hua

Mesbah

Wang

et al. 2023

Environmental Microbiology

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The moderate halophilic bacterium Alkalicoccus halolimnae BZ‐SZ‐XJ29T exhibits optimum growth over a wide range of NaCl concentrations (8.3–12.3%, w/v; 1.42–2.1 mol L−1). However, its adaptive mechanisms to cope with high salt‐induced osmotic stress remain unclear. Using TMT‐based quantitative proteomics, the cellular proteome was assessed under low (4% NaCl, 0.68 mol L−1 NaCl, control (CK) group), moderate (8% NaCl, 1.37 mol L−1 NaCl), high (12% NaCl, 2.05 mol L−1 NaCl), and extremely high (16% NaCl, 2.74 mol L−1 NaCl) salinity conditions. Digital droplet PCR confirmed the transcription of candidate genes related to salinity. A. halolimnae utilized distinct adaptation strategies to cope with different salinity conditions. Mechanisms such as accumulating different amounts and types of compatible solutes (i.e., ectoine, glycine betaine, glutamate, and glutamine) and the uptake of glycine betaine and glutamate were employed to cope with osmotic stress. Ectoine synthesis and accumulation were critical to the salt adaptation of A. halolimnae. The expression of EctA, EctB, and EctC, as well as the intracellular accumulation of ectoine, significantly and consistently increased with increasing salinity. Glycine betaine and glutamate concentrations remained constant under the four NaCl concentrations. The total content of glutamine and glutamate maintained a dynamic balance and, when exposed to different salinities, may play a role in low salinity‐induced osmoadaptation. Moreover, cellular metabolism was severely affected at high salt concentrations, but the synthesis of amino acids, carbohydrate metabolism, and membrane transport related to haloadptation was preserved to maintain cytoplasmic concentration at high salinity. These findings provide insights into the osmoadaptation mechanisms of moderate halophiles and can serve as a theoretical underpinning for industrial production and application of compatible solutes.

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Section: Ectoine and Hydroxyectoine Play A Major Role In Salt Adaptationsupporting

confidence: 92%

Section: Effect Of Salt Stress On Cellular Metabolism and Membrane Tr...supporting

confidence: 75%

Section: Ectoine and Hydroxyectoine Play A Major Role In Salt Adaptationcontrasting

confidence: 71%

Section: Ectoine and Hydroxyectoine Play A Major Role In Salt Adaptationmentioning

confidence: 95%

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Tandem mass tag‐based quantitative proteomics reveals osmotic adaptation mechanisms in Alkalicoccus halolimnae BZ‐SZ‐XJ29^T, a halophilic bacterium with a broad salinity range for optimal growth

Qing-hua

Mesbah

Wang

et al. 2023

Environmental Microbiology

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“…It belongs to Halomonadaceae family [2]. Currently, there are 119 Halomonas species (https://lpsn.dsmz.de/) and interestingly, most of them are isolated from marine or hypersaline habitats [3]. They are slightly to moderately halophilic and oligotrophic organisms [4].…”

Section: Introductionmentioning

confidence: 99%

Genome Analysis Provides Insights into the Osmoadaptation Mechanisms of Halomonas titanicae

Najjari¹

2023

Life in Extreme Environments - Diversity, Adaptability and Valuable Resources of Bioactive Molecules

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Here, we report the osmoadaptation strategies adopted by the halotolerant species Halomonas titanicae BH1(T) inferred from genome sequence analysis. BH strain was isolated in 2010 from a rusticated sample collected in 1991 from the wreck of the Titanic, genome deposited in the database under the accession number (CP059082.1). It showed a high salt tolerance ranging from 0.5 to 25% NaCl (w/v) (optimal growth at 10% NaCl) with no growth in the absence of NaCl. The phylogenomic analysis showed that the BH1 strain is more closely related to the Halomonas sedementi QX-2, a strain isolated from deep-sea sediments. The RAST (Rapid Annotation using Subsystem Technology) annotation revealed divergent mechanisms involved in the primary and secondary response to osmotic stress citing protein implicated in potassium transport, periplasmic glucan synthesis, choline and betaine upake system, biosynthesis of glycine-betaine, ectoine, and proline. These findings provide an overview of the osmoadaptive mechanisms of H. titanicae BH1, and could offer helpful information to future biotechnological applications like osmolyte synthesis and related applications.

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Quantitative evaluation of endogenous reference genes for ddPCR under salt stress using a moderate halophile

et al. 2023

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Study of osmoadaptation mechanisms of halophilic Halomonas alkaliphila XH26 under salt stress by transcriptome and ectoine analysis

Cited by 10 publications

References 67 publications

Tandem mass tag‐based quantitative proteomics reveals osmotic adaptation mechanisms in Alkalicoccus halolimnae BZ‐SZ‐XJ29^T, a halophilic bacterium with a broad salinity range for optimal growth

Tandem mass tag‐based quantitative proteomics reveals osmotic adaptation mechanisms in Alkalicoccus halolimnae BZ‐SZ‐XJ29^T, a halophilic bacterium with a broad salinity range for optimal growth

Genome Analysis Provides Insights into the Osmoadaptation Mechanisms of Halomonas titanicae

Quantitative evaluation of endogenous reference genes for ddPCR under salt stress using a moderate halophile

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Study of osmoadaptation mechanisms of halophilic Halomonas alkaliphila XH26 under salt stress by transcriptome and ectoine analysis

Cited by 10 publications

References 67 publications

Tandem mass tag‐based quantitative proteomics reveals osmotic adaptation mechanisms in Alkalicoccus halolimnae BZ‐SZ‐XJ29T, a halophilic bacterium with a broad salinity range for optimal growth

Tandem mass tag‐based quantitative proteomics reveals osmotic adaptation mechanisms in Alkalicoccus halolimnae BZ‐SZ‐XJ29T, a halophilic bacterium with a broad salinity range for optimal growth

Genome Analysis Provides Insights into the Osmoadaptation Mechanisms of Halomonas titanicae

Quantitative evaluation of endogenous reference genes for ddPCR under salt stress using a moderate halophile

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Tandem mass tag‐based quantitative proteomics reveals osmotic adaptation mechanisms in Alkalicoccus halolimnae BZ‐SZ‐XJ29^T, a halophilic bacterium with a broad salinity range for optimal growth

Tandem mass tag‐based quantitative proteomics reveals osmotic adaptation mechanisms in Alkalicoccus halolimnae BZ‐SZ‐XJ29^T, a halophilic bacterium with a broad salinity range for optimal growth