Study of oxidation by chemiluminescence. IV. Detection of low levels of lipid hydroperoxides by chemiluminescence

Yamamoto, Yorihiro; Niki, Etsuo; Tanimura, Ritsuko; Kamiya, Yoshio

doi:10.1007/bf02541836

Cited by 29 publications

(11 citation statements)

References 21 publications

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“…25-Hydroxy-24-methylcholesterol, lupeol, gramisterol, citrostadienol, cycloartenol were uniquely found only in CBO. No significant differences in composition and amounts of phytosterols (1,5) Campesterol 232,5 (13,2) 97,5 (1,8) 109,6 (9,3) 35,3 (4,6) 82,6 (4,8) -198,0 (24,8) Stigmasterol 2,4 (0,3) 31,4 (1,2) 9,4 (0,1) 6,5 (1,1) -18,5 (3,0) b-Sitosterol 324,7 (2,8) 162,5 (6,9) 361,3 (32,5) 645,8 (0,5) 80,7 (12,6) -559,5 ( were found in the rest of the analyzed oil samples. b-Sitosterol and campesterol were the dominant phytosterols found in these oils.…”

Section: Resultsmentioning

confidence: 99%

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Chemical Composition and Oxidative Stability of Selected Plant Oils

Szterk

Roszko

Sosińska

et al. 2010

J Americ Oil Chem Soc

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Scientific data on the oxidative stability of borage oil, Camelina sativa oil, linseed oil, evening primrose oil and pumpkin seed oil are scarce. Chemiluminescence (CL) methods most commonly used to determine the oxidative stability of oils include measurement of hydroperoxide, intensity of light emitted during the accelerated oxidation process performed at high ([100°C) temperatures or assisted by forced flow of air/oxygen through the sample. The aim of this study was to investigate the chemical composition and oxidative stability of selected vegetable oils available on the Polish market. Oxidative stability was determined using a fast, novel chemiluminescence-based method, in which light emitted during oxidation process conducted at 70°C in the presence of some catalyzing Fe 2? ions is measured. A reaction of the applied type has not been reported so far. High contents of tocopherols and phytosterols were found in the analyzed oil samples. Oxidative stability of the samples was in most cases higher than the stability of refined rapeseed oil, a relatively stable substance from the oxidation point of view.

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Section: Resultsmentioning

confidence: 99%

“…A 2-ml sample of 30% H 2 O 2 water solution titrated iodometrically [32] was dissolved in 100 ml of distilled water. Then, 90 ml of the mixture was diluted to 1,000 ml with distilled water.…”

Section: Calibration Curvementioning

confidence: 99%

Chemical Composition and Oxidative Stability of Selected Plant Oils

Szterk

Roszko

Sosińska

et al. 2010

J Americ Oil Chem Soc

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“…Several CL methods for detection of LOOH are reported in the literature; however, they provide either rapid qualitative data (3)(4)(5) or slower quantitative data (6)(7)(8). The use of HPLC combined with postcolumn CL detection has allowed the quantification of various hydroperoxides at the picomole level using a microperoxidase/luminol assay (9,10). Similar reports can be found in the literature for methyl linoleate using a heme/ luminol assay (8,11) and a cytochrome c/luminol assay (7).…”

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confidence: 74%

Development of a flow injection chemiluminescent assay for the quantification of lipid hydroperoxides

Bunting

Gray

2003

J Americ Oil Chem Soc

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An automated flow injection chemiluminescence (FICL) system for measuring lipid hydroperoxide (LOOH) concentrations in oils was developed. Initially, a crude oil-in-water emulsion (formed by mixing solvent-diluted oil with the aqueous-based CL compound, luminol, and the catalyst for the reaction, cytochrome c) was tested. The assay was rapid (60 samples per hour), reproducible (CV no greater than 10%, n = 3) and had a low sample requirement (1 mg of oil) because of its high sensitivity (0.5 nmol LOOH). CL intensity was influenced by the amount and type of oil under analysis. Owing to these factors, quantitative data were attainable only with a uniform oil concentration and with a calibrant derived from an oil equivalent to that under analysis. This method yielded quantitative data in good agreement with an iodometric titration assay for LOOH (r = 0.9204). A refinement of the first method consisted of replacing the luminol and cytochrome c CL compounds with lucigenin, resulting in an assay insensitive to α-tocopherol. A monophasic reaction solution was devised to remove the effect of turbidity; however, the CL signal was still influenced by oil type. Therefore, quantitative data were still attainable only when the same type of oil was used for calibration.Paper no. J10460 in JAOCS 80, 951-955 (October 2003).

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“…ROOH-derived chemiluminescence has been measured from plant leaves with a sensitive camera [446]. ROOH-derived chemiluminescence resulting from sodium hypochlorite-induced degradation of ROOH was proposed as a sensitive assay for ROOH [457]. In plant material, addition of acridine in reaction medium significantly improved the linear correlation between luminescence intensity and amount of ROOHs [458].…”

Section: Chemiluminescence and Thermoluminescencementioning

confidence: 99%