Study of Paclitaxel-Treated HeLa Cells by Differential Electrical Impedance Flow Cytometry

Kirkegaard, Julie; Clausen, Chris; Rodriguez-Trujíllo, Romén; Svendsen, Winnie Edith

doi:10.3390/bios4030257

Cited by 24 publications

(25 citation statements)

References 23 publications

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“…Cisplatin is rendered as one of the most effective anticancer drug, especially against several types of tumors. Paclitaxel is also reported to be a potent chemotherapeutic drug which causes torn membranes in HeLa cells 29,30 . It has been seen that cancer cells develop an ability of survival against a wide range of anticancer drugs, including cisplatin, doxorubicin, vinblastine, paclitaxel etc.…”

Section: Introductionmentioning

confidence: 99%

Theranostic combinatorial drug-loaded coated cubosomes for enhanced targeting and efficacy against cancer cells

et al. 2020

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Cubosomes, a product of nanobioengineering, are self-structured lipid nanoparticles that act like drug-loaded theranostic probes. Here, we describe a simple method for the preparation of combinatorial drug-loaded cubosomes with, proof-of-principle, therapeutic effect against cancer cells, along with diagnostic capabilities. Anticancer drugs cisplatin and paclitaxel were loaded in the cubosomes in combination. The cubosomes were coated with a layer of poly-Ɛ-lysine, which helped avoid the initial burst release of drug and allowed for a slow and sustained release for better efficacy. Cubosomes were imaged by transmission electron microscope, and their dispersion analyzed in vitro by differential scanning calorimetric and X-ray diffractogram studies. The microscopic images depicted spherical polyangular structures, which are easily distinguishable. The analyses revealed that the drug is uniformly dispersed all through the cubosomes. Further characterization was carried out by zeta-potential measurement, in vitro release, and entrapment efficiency studies. The in vitro studies established that the coating of cubosomes successfully reduced the burst release of drugs initially and confirmed a slow, sustained release over increased time. Comparative cytotoxicity of coated, uncoated, and blank cubosomes was evaluated, using human hepatoma HepG2 cell line, and the formulations were found to be entirely nontoxic, similar to the blank ones. The therapeutic efficiency of the cubosomes against HeLa cells was confirmed by the impedance measurement and fluorescent imaging. Furthermore, the reduction in impedance in cells treated with coated combinatorial cubosomes proved the impairment of HeLa cells, as confirmed by fluorescence microscopy.

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Section: Introductionmentioning

confidence: 99%

Theranostic combinatorial drug-loaded coated cubosomes for enhanced targeting and efficacy against cancer cells

et al. 2020

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“…They reported the capability of EIS to differentiate erythrocytes and erythrocyte ghost cells, as well as solid particle size separation in continuous flow. Furthermore, the technology has been applied to a broad number of different micro-sized samples; it has been used to distinguish between different yeast cells [ 14 ] and human blood cells of different kinds [ 9 , 15 , 16 , 17 ]. EIS has also been used to measure the effect of electrical lysis on yeast cells [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

Bacteria Detection and Differentiation Using Impedance Flow Cytometry

Clausen¹,

Dimaki

Bertelsen

et al. 2018

Sensors

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Monitoring of bacteria concentrations is of great importance in drinking water management. Continuous real-time monitoring enables better microbiological control of the water and helps prevent contaminated water from reaching the households. We have developed a microfluidic sensor with the potential to accurately assess bacteria levels in drinking water in real-time. Multi frequency electrical impedance spectroscopy is used to monitor a liquid sample, while it is continuously passed through the sensor. We investigate three aspects of this sensor: First we show that the sensor is able to differentiate Escherichia coli (Gram-negative) bacteria from solid particles (polystyrene beads) based on an electrical response in the high frequency phase and individually enumerate the two samples. Next, we demonstrate the sensor’s ability to measure the bacteria concentration by comparing the results to those obtained by the traditional CFU counting method. Last, we show the sensor’s potential to distinguish between different bacteria types by detecting different signatures for S. aureus and E. coli mixed in the same sample. Our investigations show that the sensor has the potential to be extremely effective at detecting sudden bacterial contaminations found in drinking water, and eventually also identify them.

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“…Thus, previous research assumed that cellular responses to frequency changes would be linear and unrelated to the direction of variation. However, many reports have described nonlinear electrodynamic responses as a function of various external stimuli and internal cell signal processes [ 16 , 28 , 29 , 30 , 31 , 32 ]. Therefore, cellular DEP behaviors could vary if the input frequency is successively increased and decreased.…”

Section: Introductionmentioning

confidence: 99%

Non-Linear Cellular Dielectrophoretic Behavior Characterization Using Dielectrophoretic Tweezers-Based Force Spectroscopy inside a Microfluidic Device

Choi

Lim

et al. 2018

Sensors

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Characterization of cellular dielectrophoretic (DEP) behaviors, when cells are exposed to an alternating current (AC) electric field of varying frequency, is fundamentally important to many applications using dielectrophoresis. However, to date, that characterization has been performed with monotonically increasing or decreasing frequency, not with successive increases and decreases, even though cells might behave differently with those frequency modulations due to the nonlinear cellular electrodynamic responses reported in previous works. In this report, we present a method to trace the behaviors of numerous cells simultaneously at the single-cell level in a simple, robust manner using dielectrophoretic tweezers-based force spectroscopy. Using this method, the behaviors of more than 150 cells were traced in a single environment at the same time, while a modulated DEP force acted upon them, resulting in characterization of nonlinear DEP cellular behaviors and generation of different cross-over frequencies in living cells by modulating the DEP force. This study demonstrated that living cells can have non-linear di-polarized responses depending on the modulation direction of the applied frequency as well as providing a simple and reliable platform from which to measure a cellular cross-over frequency and characterize its nonlinear property.

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Study of Paclitaxel-Treated HeLa Cells by Differential Electrical Impedance Flow Cytometry

Cited by 24 publications

References 23 publications

Theranostic combinatorial drug-loaded coated cubosomes for enhanced targeting and efficacy against cancer cells

Theranostic combinatorial drug-loaded coated cubosomes for enhanced targeting and efficacy against cancer cells

Bacteria Detection and Differentiation Using Impedance Flow Cytometry

Non-Linear Cellular Dielectrophoretic Behavior Characterization Using Dielectrophoretic Tweezers-Based Force Spectroscopy inside a Microfluidic Device

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