Study of patient's injuries by stingrays, lethal activity determination and cardiac effects induced by Himantura gerrardi venom

Dehghani, Hadi; Sajjadi, Mir Masoud; Rajaian, Hamid; Sajedianfard, Javad; Parto, Paria

doi:10.1016/j.toxicon.2009.06.023

Cited by 17 publications

(10 citation statements)

References 22 publications

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“…In Iranian coasts, the rate of stingray envenomation seems to be relatively high, but very few were referred to the clinical centers. It is probably the position of injuries that are often in limbs which do not cause serious symptoms and may be alleviated even without any specific and non specific treatments [24].…”

Section: Discussionmentioning

confidence: 99%

Fibrinogenolytic and anticoagulant activities in the tissue covering the stingers of marine stingrays Dasyatis sephen and Aetobatis narinari

Kumar

Vennila

Kanchana

et al. 2010

J Thromb Thrombolysis

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Stingray envenomation is one of the major problems in the marine and freshwater ecosystem. Accidents in human cause immediate, local and intense pain, erythema, edema, hemorrhage, tissue necrosis and secondary bacterial infection are also common. To determine the effect of two marine stingray species Dasyatis sephen and Aetobatis narinari venom extract on coagulation, fibrin(ogen)olytic, proteolytic activities. Plasma coagulation, Thrombin catalyzed fibrinocoagulation, Fibrin plate assay, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), substrate SDS-PAGE and thrombin like activity by using chromogenic substrate were used to determine the effect of venom on plasma coagulation, its fibrin(ogen)olytic and proteolytic activity. The results show the presence of fibrin(ogen)olytic, anticoagulant and gelatinolytic activity in both stingray venom extracts. D. sephen venom delays coagulation of citrated plasma more significantly than A. narinari upon using increasing concentration of the venom. The same results were obtained in the fibrinocoagulation assays. SDS-PAGE analysis of fibrinogen and fibrin after incubation with D. sephen and A. narinari venom show fibrin(ogen)olytic activity. Through SDS-PAGE analysis it is confirmed that the delaying in coagulation process by stingray venom is due to its fibrin(ogen)olytic activity and fibrinolytic activity also confirmed through fibrin plate assay. Zymogram analysis shows the presence of array of gelatinolytic and fibrinogenolytic enzymes above 43-276 kDa in the D. sephen and A. narinari venom respectively. Protease inhibitor studies show the serine and metallo proteases are responsible for these activities. From the results, fibrinogenolytic, proteolytic activity of the stingray venom is confirmed, but it has no thrombin like activity and these activities may aid in hemorrhages, tissue necrosis and secondary bacterial infections at the site of envenomation.

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Section: Discussionmentioning

confidence: 99%

Fibrinogenolytic and anticoagulant activities in the tissue covering the stingers of marine stingrays Dasyatis sephen and Aetobatis narinari

Kumar

Vennila

Kanchana

et al. 2010

J Thromb Thrombolysis

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“…Stingrays may have one to seven spines on their whip-like tail, which are responsible for numerous injuries worldwide [17][18][19]. Their spines are hard, sharp, bilaterally retroserrated and covered by an integumentary sheath with a ventrolateral glandular groove containing venom glands along either edge [5].…”

Section: Introductionmentioning

confidence: 99%

Studies on pharmacological properties of mucus and sting venom of Potamotrygon cf. henlei

Monteiro-dos-Santos

Conceição

Seibert

et al. 2011

International Immunopharmacology

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Stingrays from the Potamotrygon cf. henlei species are widely distributed in high numbers throughout the rivers of central-west Brazil, being the source of numerous envenomations occurring in the dry season, posing a serious public health problem even if not properly reported. The accidents usually involve fishermen and bathers, and to date there is no effective treatment for the injured. Considering these facts and limitations of studies aiming at understanding the effects induced by P. cf. henlei envenoming, this study aimed to describe the principal pharmacological and certain biochemical properties of the mucus and sting venom. We found that mucus and sting venom is toxic to mice having nociceptive, edematogenic and proteolysis activities. Our results also indicate that the inflammatory cellular influx observed could be triggered by the venom and mucus. Furthermore the venom and mucus were partially purified by solid-phase extraction tested for antimicrobial activity in which only the mucus presented activity. It could be inferred from the present study that P. cf. henlei venom possesses a diverse mixture of peptides, enzymes and pharmacologically active components.

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“…On impalement, the secreting cells are unroofed and the venom diffuses into the wound causing damaging effects locally through vasoconstriction, leading to ischemia and necrosis. 8,9 The most frequent symptoms/signs after an injury are local pain, swelling, erythema, bruising, and inflammation. Generalized symptoms such as nausea, abdominal pain, shortness of breath, and signs of cardiac arrhythmias are rare (< 5%).…”

Section: Discussionmentioning

confidence: 99%

Case Report: Iatrogenic Infection from Traditional Treatment of Stingray Envenomation

Hønge

Patsche

Jensen

et al. 2018

The American Journal of Tropical Medicine and Hygiene

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A 47-year-old man was stung on the left ankle by a stingray while on vacation on the Island of Bubaque, Guinea-Bissau. The affected limb was initially treated with an attempt to suck out the venom and application of chewed plant root. The following 3 days, local pain gradually diminished, but then high fever erupted together with generalized symptoms and intense pain from the ankle. After initiating antibiotic treatment, the patient was evacuated. Because of sustained symptoms and fever, the wound was surgically debrided, and culture revealed infection with oral flora bacteria. Attempts to suck out venom are not recommended.

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Study of patient's injuries by stingrays, lethal activity determination and cardiac effects induced by Himantura gerrardi venom

Cited by 17 publications

References 22 publications

Fibrinogenolytic and anticoagulant activities in the tissue covering the stingers of marine stingrays Dasyatis sephen and Aetobatis narinari

Fibrinogenolytic and anticoagulant activities in the tissue covering the stingers of marine stingrays Dasyatis sephen and Aetobatis narinari

Studies on pharmacological properties of mucus and sting venom of Potamotrygon cf. henlei

Case Report: Iatrogenic Infection from Traditional Treatment of Stingray Envenomation

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