Study of penetration mechanism of labrasol on rabbit cornea by Ussing chamber, RT-PCR assay, Western blot and immunohistochemistry

Guo, Pan; Li, Nan; Fan, Lili; Lü, Jun; Liu, Boying; Zhang, Bing; Wu, Yumei; Liu, Zhidong; Li, Jiawei; Pi, Jiaxin; Qi, Dongli

doi:10.1016/j.ajps.2018.05.005

Cited by 14 publications

(6 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Data analysis was performed using the 2 −ΔΔCT method and normalized with the housekeeping gene ACTB. The sequences of RT-qPCR primers were as follows (Meiji Biotechnology): peroxisome proliferator-activated receptor β (PPARB; Sanchez et al, 2017) The specific experimental method was carried out with reference to Guo et al (2019). The expression of PPARB and CEBPB genes were verified with ACTB as control.…”

Section: Detection Of Animal Sample Indicatorsmentioning

confidence: 99%

Effects of skim milk fermented with Lactobacillus plantarum WW on the constitutions of rats fed a high-fat diet

Cao

et al. 2020

Journal of Dairy Science

View full text Add to dashboard Cite

This study investigated the effects of Lactobacillus plantarum WW-fermented skim milk (FSM) on the physiques of rats fed a high-fat diet and the mechanism of lipid lowering. Sprague-Dawley rats were randomly divided into a normal diet group (A), a high-fat diet group (B), a skim milk diet group (C), and an L. plantarum WW FSM diet group (D). After 12-wk feeding, we found that treatment with L. plantarum WW FSM could significantly alleviate symptoms in the pathological group. Meanwhile, high-throughput sequencing analysis showed that L. plantarum WW FSM also had a certain regulatory effect on the intestinal microorganisms in rats, which can increase the number of lactic acid bacteria and Bacteroides in the intestine. More importantly, real-time quantitative PCR and Western blot analysis showed that the probiotic was also involved in the expression of genes related to fat metabolism, especially the PPARB and CEBPB genes. Our study supports the hypothesis that the WW strain of L. plantarum could be a potential probiotic to be used in functional foods to alter lipid metabolism and reduce cholesterol levels.

show abstract

Section: Detection Of Animal Sample Indicatorsmentioning

confidence: 99%

Effects of skim milk fermented with Lactobacillus plantarum WW on the constitutions of rats fed a high-fat diet

Cao

et al. 2020

Journal of Dairy Science

View full text Add to dashboard Cite

show abstract

“…Such microphysiological systems are highly customisable and can be tailored to the design of the experiment. While it could be argued that diffusion chambers such as Franz cells 76 and Ussing chambers 77 chip and has been used to validate the efficacy of a novel compound. 78 Corneas-on-chips, however, are still in their infancy and a long road lies ahead before they can be used for robust pharmacological testing.…”

Section: Discussionmentioning

confidence: 99%

An Overview of Advanced In Vitro Corneal Models: Implications for Pharmacological Testing

Meenen

Dhubhghaill

Bogerd

et al. 2022

Tissue Engineering Part B: Reviews

View full text Add to dashboard Cite

The cornea is an important barrier to consider when developing ophthalmic formulations, but proper modelling of this multi-layered tissue remains a challenge. This due to the varying properties associated with each layer in addition to the dynamics of the tear film.Hence, the most representative models to date rely on animals. Animal models, however, differ from humans in several aspects and are subject to ethical limitations. Consequently, in vitro approaches are being developed to address these issues. This review focusses on the barrier properties of the cornea and evaluates the most advanced 3D cultures of human corneal equivalents in literature. Their application potential is subsequently assessed and discussed in the context of preclinical testing along with our perspective towards the future.

show abstract

“…Hans Ussing initially developed the Ussing chamber to measure the transport of nutrients and ions across various epithelial membranes . More recently, modified Ussing chambers such as the Navicyte/Harvard Ussing system have been used in corneal drug diffusion studies. − It is designed in such a way that each side of the cornea faces another chamber-half, with the cornea being positioned between the chamber halves using a series of pins surrounding the opening (Figure B). The addition of electrodes allows for the performance of electrophysiological measurements, such as the measurement of corneal transepithelial electrical resistance.…”

Section: Ex Vivo Animal Modelsmentioning

confidence: 99%

In Vitro and Ex Vivo Models for Assessing Drug Permeation across the Cornea

et al. 2023

View full text Add to dashboard Cite

Drug permeation across the cornea remains a major challenge due to its unique and complex anatomy and physiology. Static barriers such as the different layers of the cornea, as well as dynamic aspects such as the constant renewal of the tear film and the presence of the mucin layer together with efflux pumps, all present unique challenges for effective ophthalmic drug delivery. To overcome some of the current ophthalmic drug limitations, the identification and testing of novel drug formulations such as liposomes, nanoemulsions, and nanoparticles began to be considered and widely explored. In the early stages of corneal drug development reliable in vitro and ex vivo alternatives, are required, to be in line with the principles of the 3Rs (Replacement, Reduction, and Refinement), with such methods being in addition faster and more ethical alternatives to in vivo studies. The ocular field remains limited to a handful of predictive models for ophthalmic drug permeation. In vitro cell culture models are increasingly used when it comes to transcorneal permeation studies. Ex vivo models using excised animal tissue such as porcine eyes are the model of choice to study corneal permeation and promising advancements have been reported over the years. Interspecies characteristics must be considered in detail when using such models. This review updates the current knowledge about in vitro and ex vivo corneal permeability models and evaluates their advantages and limitations.

show abstract

Study of penetration mechanism of labrasol on rabbit cornea by Ussing chamber, RT-PCR assay, Western blot and immunohistochemistry

Cited by 14 publications

References 32 publications

Effects of skim milk fermented with Lactobacillus plantarum WW on the constitutions of rats fed a high-fat diet

Effects of skim milk fermented with Lactobacillus plantarum WW on the constitutions of rats fed a high-fat diet

An Overview of Advanced In Vitro Corneal Models: Implications for Pharmacological Testing

In Vitro and Ex Vivo Models for Assessing Drug Permeation across the Cornea

Contact Info

Product

Resources

About