Study of plasma-induced peripheral blood mononuclear cells survival using Fourier transform infrared microspectroscopy

Abstract

Abstract. Components present in the acellular fraction of blood influence the blood cell survival and function and the response to biotic and abiotic factors. Human plasma and sera have been used as therapeutic agents and are known to increase cell survival. White blood cells in normal blood are exposed to plasma components in vivo, but the effect of such plasma components in vitro on adherent peripheral blood mononuclear cells (PBMCs) that includes monocytes has not been fully investigated. We cultured human PBMCs with autologous plasma and observed structural variation due to plasma addition in PBMCs along with increased cell survival. Light microscopy of the cells showed increased granularity in plasma-treated cells. Fourier transform infrared (FTIR) spectroscopy was used to elucidate the possible mechanism by studying the changes in the biochemical composition of the cells that explained the observations. FTIR spectroscopy of plasma-treated cells show altered spectral pattern in the mid-IR region, indicating increased phospholipid levels. Heat-stable components in the plasma possibly increase the differentiation of PBMCs, as evident by increased phospholipid metabolism. The data suggest that plasma-stimulated membrane biogenesis may contribute to PBMC survival by inducing them to differentiate into antigen presenting cells (APCs) like macrophages and dendritic cells.

Using Infrared Spectroscopy and Multivariate Analysis to Detect Antibiotics’ Resistant Escherichia coli Bacteria

“…In qualitative and quantitative analytical applications, the potential of IR spectroscopy to identify chemical components by analysis of their vibrational spectra fingerprints can be of great value. IR spectroscopy is commonly used in the fields of biochemistry, biomedical science, materials science, and medicine, where its value has been demonstrated. − The mid-infrared spectral region is rich with structural and functional information regarding the cell’s molecular components, ,, which enables the technique to detect molecular changes related to early stages of the development of cancer, for instance refs and . Moreover, earlier studies showed that IR spectroscopy was able to detect cancer cells at very early stages of the cancer, even when the histological appearance of the cells still looks normal. ,, Despite the completely different biochemistry of cancer development in eukaryotic cells vs resistivity to antibiotics in prokaryotes, both transformations are the result of complicated and subtle biochemistry changes, and we submit that successful discrimination by FT-IR of such changes in eukaryotes may give reasonable expectations for a similar success in prokaryotes.…”

Tracing overlapping biological signals in mid-infrared using colonic tissues as a model system

“…The number of representing points ( i.e ., number of different spectra) is denoted by N . In order to find the features characteristic of each category, two methods were considered: principal component analysis (PCA), and linear discriminant analysis (LDA)[12-16]. PCA is was used for dimensionality reduction[12,14,17-20], as well as for a rough classification of the measured data into the five categories.…”