Study of Serum and Tissues Angiotensin Converting Enzyme (ACE) Activity in Rat with Gentamicin Induced Renal Toxicity

Ziai, Seyed Ali; Salehian, Pirooz; Mahmoudian, Massoud

doi:10.1081/jdi-120026027

Renal Failure

2003

DOI: 10.1081/jdi-120026027

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Study of Serum and Tissues Angiotensin Converting Enzyme (ACE) Activity in Rat with Gentamicin Induced Renal Toxicity

Seyed Ali Ziai

Pirooz Salehian

Massoud Mahmoudian

Abstract: These data suggest that kidney ACE activity decreased significantly just one day after gentamicin administration and prior to kidney NAG decrease.

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Cited by 5 publications

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“…Urinary N-acetyl-β-D-glucosaminidase (NAG) levels have been reported to increase after exposure to various toxic substances, such as lead and cadmium [7], solvents [10], contrast media [8], aminoglycoside antibiotics [11], nephrotoxic drugs [4], puromycin aminonucleoside-induced glomerulonephritis [1], and various human glomerular diseases, including diabetic nephropathy [9]. Serum creatinine has limited value in the detection of early renal damage, and thus urinary enzymes such as NAG are commonly used as markers for tubular injury.…”

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confidence: 99%

Short Term Change of Urinary N-Acetyl-.BETA.-D-Glucosaminidase in Reduced Kidney Mass with Renal Artery Ligation

Ebisawa

Uechi

Hori

et al. 2006

The Journal of Veterinary Medical Science

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ABSTRACT. The aim of the present study was to evaluate short term urinary NAG levels in a model of reduced kidney mass. The half and quarter kidney mass were made from ligation of the renal artery. Both groups decreased in the level of excreted NAG on day 1 and 2 after operation. On day 5 after operation, both groups achieved urinary enzyme levels comparable to that of the sham-operated group. The remaining compensated nephrons held normal range of excreted urinary NAG levels, although reduced number of nephrons resulted in a decline in urinary NAG levels. KEY WORDS: kidney function, renal disorder, urinary enzyme.J. Vet. Med. Sci. 68(12): 1355-1357, 2006 Urinary [9]. Serum creatinine has limited value in the detection of early renal damage, and thus urinary enzymes such as NAG are commonly used as markers for tubular injury. To date, there have been no studies on urinary NAG levels in reduced kidney mass. The aim of the study was to evaluate short term urinary NAG levels in a model of reduced kidney mass with renal artery ligation.The experiments were performed on 32 male SpragueDawley rats (Charles River Japan, Inc.), with a mean weight of 294 ± 11 g. Each rat was housed in a metabolism cage with controlled temperature (23°C) and relative humidity (52 ± 13%), and a synchronized 12-hr light/dark cycle. The rats were fed food and water ad libitum. Dietary intake and body weight were measured once daily.Prior to laparectomy, the rats were anesthetized with pentobarbital 40 mg/kg. The rats were assigned to sham operation (n=9), ligation of the right renal artery and vein (1/2 kidney; n=11), or ligation of the right renal artery and vein plus the left renal branch artery (1/4 kidney; n=12).Urine samples were collected for 4 days before operation as baseline samples and at 1-5, 12, and 19 days after operation. Urine volume was measured during each collection period. One ml of collected urine was centrifuged at 3,000 rpm for 5 min to obtain the supernatant, which was immediately stored at 4°C and used for measurement within 48 hr. At 3 days before operation and at 5, 12, and 19 days after operation, 1 ml of blood was collected from the subclavian vein under light anesthesia. The blood was centrifuged at 3,000 rpm for 5 min to obtain the supernatant, which was frozen and stored.The activity of urinary NAG was determined colorimetrically as the absorbance at 580 nm wavelength of m-cresol purple released by NAG from the substrate, sodio m-cresolsulfonphthaleinyl-N-acetyl-β-D-glucosaminide (MCP-NAG) (Shionogi & Co., Ltd., Tokyo, Japan). Serum and urine creatinine levels were measured with the Jáffe reaction method using a commercially available kit (Chugai Pharmaceutical Co., Ltd., Tokyo, Japan).Enzyme excretion (Eext) was calculated from measures of urinary enzyme activity (Eact) and urine volume (Uvol): Eext (U/kg) = Eact (U/l) × Uvol (ml)/kg body weight.The data collected at the different time points were analyzed for differences in body weight, water intake, food intake, urine volume, creatinine excretion, sodium ex...

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confidence: 99%

Short Term Change of Urinary N-Acetyl-.BETA.-D-Glucosaminidase in Reduced Kidney Mass with Renal Artery Ligation

Ebisawa

Uechi

Hori

et al. 2006

The Journal of Veterinary Medical Science

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Heart Angiotensin-Converting Enzyme Activity Increased After Intraperitoneal Bleomycin Administration in Rat

Ziai

Salehian

Mahmoudian

2004

Experimental Lung Research

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Angiotensin-converting enzyme (ACE) generates angiotensin II and degrades bradykinin. This ectopeptidase acts as a marker in pathologic conditions, showing tissue and vascular damage. In order to evaluate serum and tissue ACE activity in bleomycin-associated lung damage, the authors studied morphological and enzymatic alterations as well as blood pressure in male Sprague-Dawley rats. Animals were treated intraperitoneally with bleomycin (5 mg/kg) 3 times for 1 week. This subacute and therapeutic dose produced morphologically evident pulmonary damage characterized by alveolar septum thickness and collagen deposition. Bleomycin also had significantly lowered lung and aorta N-acetyl-beta-D-glucosaminidase (NAG) activity, whereas heart and serum NAG activity increased significantly. Serum ACE activity was significantly lower in the treated animals, and heart ACE activity increased significantly. Lung ACE activity and blood pressure did not change during the study. The authors conclude that heart ACE activity increased faster than pulmonary ACE and serum ACE is a good indicator of damage even in subacute therapeutic bleomycin doses.

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Differences in acute kidney injury ascertainment for clinical and preclinical studies

Fiorentino

Castellano

Kellum

2017

Nephrology Dialysis Transplantation

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Study of Serum and Tissues Angiotensin Converting Enzyme (ACE) Activity in Rat with Gentamicin Induced Renal Toxicity

Abstract: These data suggest that kidney ACE activity decreased significantly just one day after gentamicin administration and prior to kidney NAG decrease.

Cited by 5 publications

References 34 publications

Short Term Change of Urinary N-Acetyl-.BETA.-D-Glucosaminidase in Reduced Kidney Mass with Renal Artery Ligation

Short Term Change of Urinary N-Acetyl-.BETA.-D-Glucosaminidase in Reduced Kidney Mass with Renal Artery Ligation

Heart Angiotensin-Converting Enzyme Activity Increased After Intraperitoneal Bleomycin Administration in Rat

Differences in acute kidney injury ascertainment for clinical and preclinical studies

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