Study of sex determination system in ship sturgeon, Acipenser nudiventris using meiotic gynogenesis

Saber, Mohammad Hassanzadeh; Hallajian, Ali

doi:10.1007/s10499-013-9676-z

Cited by 23 publications

(10 citation statements)

References 16 publications

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“…By measuring the sex ratio of gynogenetic diploids, the sex determination system can be determined (Campos‐Ramos et al., ; Chen et al., ; Hassanzadeh Saber & Hallajian, ; Komen & Thorgaard, ). Therefore, gynogenesis is a useful technique for studies on sex determination mechanism in fish.…”

Section: Discussionmentioning

confidence: 99%

“…Each UV treatment experiment was replicated three times. The entire process was carried out in the dark to prevent genetic photoreactivation (Hassanzadeh Saber & Hallajian, ). The rates of fertilization, hatching and larvae surviving were respectively determined as in the previous description (Ji et al., ; You et al., ).…”

Section: Methodsmentioning

confidence: 99%

“…The techniques are relatively simple with two steps: generally, inactivation of spermatozoa by UV irradiation or chemical treatments and fertilized egg shock treatments (thermal or pressure) for diploidization of maternal chromosome set (Ghigliotti, Bolla, Duc, Ottesen & Babiak, ; Lebeda, Dzyuba, Rodina & Flajshans, ; Nowosad, Kucharczyk, Liszewski, Targońska & Kujawa, ; You, Yu, Tan & Tong, ). Artificial gynogenesis has been widely used in the study of fish genetics and breeding like for the production of monosex fish (Luo et al., ; Purdom, ), establishment of inbred lines (Li, Liang, Luo, Pan & Zou, ) and identification of the sex determination system (Campos‐Ramos, Harvey, Mcandrew & Penman, ; Chen et al., ; Fopp‐Bayat, ; Hassanzadeh Saber & Hallajian, ; Li et al., ; Luo et al., ; Purdom, ).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, sex identification through the cytogenetical method is rarely possible. By scoring sex ratios of successfully induced gynogens, sex determination modes of some fishes have been clarified: in Mozambique tilapia ( Oreochromis mossambicus ) (Campos‐Ramos et al., ), stinging catfish ( Heteropneustes fossilis ) (Christopher, Murugesan & Sukumaran, ) and turbot ( Scophthalmus maximus ) (Piferrer et al., ), sex determination was proven as the XX/XY pattern, while in paddlefish ( Polyodon spathula ) (Shelton & Mims, ), half‐smooth tongue sole ( Cynoglossus semilaevis ) (Chen et al., ), large‐scale loach ( Paramisgurnus dabryanus ) (You et al., ), ship sturgeon ( Acipenser nudiventris ) (Hassanzadeh Saber & Hallajian, ), Siberian sturgeon ( Acipenser baeri ) (Fopp‐Bayat, ), rosy bitterling ( Rhodeus ocellatus ocellatus ) (Kawamura, ) and turbot ( Scophthalmus maximus ) (Meng et al., ),ZZ/ZW was believed to be the sex determination type.…”

Section: Introductionmentioning

confidence: 99%

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Induction of meiotic gynogenesis in bagrid catfish (Pseudobagrus ussuriensis) with homologous sperm and its confirmation for female homogamety

et al. 2017

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The bagrid catfish, Pseudobagrus ussuriensis, exhibits significant sexual dimorphism in growth rate and body size with males growing faster than females. Therefore, an all‐male culture can dramatically increase the output and profitability of fishery products. According to the monosex breeding route, super‐male individuals’ acquirement by XY male sex reversal and artificial gynogenesis is the key step. An effective protocol to induce meiotic gynogenesis using homologous sperms has been developed in this study. The most effective UV irradiation for sperm genetic inactivation was found to be at a distance of 20 cm with 66 μW/cm2 light intensity for 25 min. Optimal treatment for cold shock was 5 min post‐fertilization at 0‐4°C for 30 min, which gave the best survival rate of 13.65 ± 2.87%. The sex ratio in the meiotic gynogens showed a significant female‐biased deviation (p < .05); thirty meiogynogens and their parents were further analysed using a male‐specific AFLP marker, of which only the male parent produced a male‐specific DNA band of 412 bp. These results indicated the female homogametic XX/XY sex determination system in P. ussuriensis. The optimization of a protocol for the successful induction of meiogynogenesis in the bagrid catfish lays the basis for all‐male production and is useful in ascertaining the genetic sex determination system in this promising aquaculture species.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Induction of meiotic gynogenesis in bagrid catfish (Pseudobagrus ussuriensis) with homologous sperm and its confirmation for female homogamety

et al. 2017

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“…High maturation age and time consumed for verification of sturgeon sex reversed progeny may cause prohibition for study on their sex reversal. As sex determination system in the ship sturgeon is female heterogamety (Saber & Hallajian, 2014), it expected that masculinization in the gynogentic progeny could be suitable for all-female production from this species and other sturgeon having long time sexual maturation. The aim of this study was obtaining the optimal dose of MT hormone for successfully sex reversal of produced gynogen progeny into male and then sex controlling and shortening in all-female ship sturgeon production.…”

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confidence: 99%

Masculinization of the gynogenetic juvenile ship sturgeon (Acipenser nudiventrisLovetsky, 1828) using 17α‐methyl testosterone

et al. 2019

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In sturgeons, the induction of gynogenesis and sex reversal could be important for potential production of neomale sturgeon and all‐female progeny for caviar production. The aim of this study was sex reversal of ship sturgeon (Acipenser nudiventris Lovetsky, 1828) gynogen into male sex. Five‐month‐old gynogens were sex reversed into male by including 17α‐methyl testosterone in their food for 7 months. Three treatments were considered as follows: (a) without treated (gynogen control), (b) 10 mg MT/kg diet, and (c) 50 mg MT/kg diet. All treatments (60 individuals) were sampled both the 30 and 36 months old and their sex was determined using classical histology method of gonad. The sex ratio of the progenies in the gynogen control were 73.3% female and 26.7% male. In treatment of 10 mg MT/kg feed, 18 specimens were studied that half of them (50%) showed pseudo‐testicular structure in the female gonad. That is 50% of the specimens were intersex, 27.7% were male and 22.3% were female. All of the fish fed by 50 mg MT/kg feed had been sex reversed to male. Sexual maturation of these fish had been recognized in stage III at 36 months old. In conclusion, 50 mg MT/kg feed found as effective dose for successful sex reversal in gynogenetic ship sturgeon.

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Sex Determination in Sturgeon

Wuertz

Güralp²,

Pšenička³

et al. 2018

Sex Control in Aquaculture

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Study of sex determination system in ship sturgeon, Acipenser nudiventris using meiotic gynogenesis

Cited by 23 publications

References 16 publications

Induction of meiotic gynogenesis in bagrid catfish (Pseudobagrus ussuriensis) with homologous sperm and its confirmation for female homogamety

Induction of meiotic gynogenesis in bagrid catfish (Pseudobagrus ussuriensis) with homologous sperm and its confirmation for female homogamety

Masculinization of the gynogenetic juvenile ship sturgeon (Acipenser nudiventrisLovetsky, 1828) using 17α‐methyl testosterone

Sex Determination in Sturgeon

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