Study of the Allergizing Properties of Nanocolloid,99mTc–Al2O3 Radiopharmaceutical in Experiment

Valerievna, Varlamova Nataliya; Stasyuk, E. S.; Titskaya, Anna; Синилкин, И. Г.; Larionova, L. A.; Sherstoboyev, E.Y.; Trofimova, E. S.; Ligacheva, A.A.; Скуридин, В. С.

doi:10.17691/stm2015.7.4.09

Cited by 4 publications

(2 citation statements)

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“…[28] Albino rats weighing 200-220 g were kept on an absolute diet for 48 h without limiting drinking water. The preparation was inserted into animals orally at doses of 100 mg/kg.…”

Section: Experimental Partmentioning

confidence: 99%

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Shpychak

2017

AJP

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Introduction: The work presents the data of the literary sources review which suggests that nowadays, treatment of the urinary tract, including prostatitis and prostate adenoma, which are the most common and serious male disorders of and tend to increase their frequency, is the topical issue of the modern medicine. It is proved that the compounds of natural substances, standardized bee products in particular -honey powder (HP), propolis phenolic hydrophobic preparation (PPHP) and bee pollen (BP), are promising for the effective treatment of this disease. Materials and Methods: The results of studying the acute toxicity and safety of a new national medication of natural origin for treating the diseases of the genitourinary system, including prostatitis and prostate adenoma, which contains above named natural substances, are presented in the experimental part. Pharmacological, physiological, instrumental research methods and mathematical statistics methods have been used in the study. Results and Discussion: The analysis of morphological studies of testes and sperm functional state of rats demonstrates no change of morphological and functional indices in the experimental group compared with the control group. Basing on these studies, we can conclude that the mixture of active pharmaceutical ingredients (APIs) in the form of bee products standardized substances composition at a dose of 100 mg/kg demonstrates no toxic effects on spermatogenesis in male rats. The results of the study allergenic activity of the studied composition at the same dose indicate no hypersensitivity of immediate type reactions and no accumulation of homocytotrophic antibodies in the blood, and accordingly the no sensitizing activity, which was also confirmed by the "conjunctival test" on guinea pigs. The research of the local irritating effect of the mixture of the APIs on the mucous membrane demonstrates the lack of such effect after a single application to the eye of rabbits. Conclusions: The study of the specific toxicity of the APIs mix in the form of a bee products standardized substances composition -HP, PPHP, and BP at a dose of 100 mg/kg, demonstrated no capacity to gonadotoxic and allergenic effect. It has been found that the formula of APIs has no effect on the secretory function and the state of the gastric mucosa and no irritating action at contacting with mucous membranes of an eye. The results of the studies of pharmacological activity and specific toxicity of the bee products standard substances composition -HP, PPHP, and BP -allow to conclude that manufacturing such preparation is promising, and further preclinical researchers to obtain a permit to introduce a new preparation into clinical practice and industry are desirable.

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“…[28] Albino rats weighing 200-220 g were kept on an absolute diet for 48 h without limiting drinking water. The preparation was inserted into animals orally at doses of 100 mg/kg.…”

Section: Experimental Partmentioning

confidence: 99%

Untitled

Shpychak

2017

AJP

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“…However, similar AgNP-containing dressings were actually shown to improve the healing of burn wounds in rats over an 18 day period as compared to rats with dressings lacking AgNP, but the study only examined the localized effects (667). The GPMT has also been used to demonstrate that surface-modified FeNP, AlNP, and hydroxyapatite nanoparticles did not induce skin sensitization (668)(669)(670).…”

Section: Skin Sensitization Studiesmentioning

confidence: 99%

Metal Nanomaterials: Immune Effects and Implications of Physicochemical Properties on Sensitization, Elicitation, and Augmentation of Allergic Disease

Roach¹

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The recent surge in incorporation of engineered metallic and metal oxide nanomaterials into consumer products and their corresponding use in occupational settings has raised concerns over their potential to induce material size-specific toxicological effects. Although metal nanomaterials have been shown to induce greater degrees of lung injury and inflammation than their larger metal counterparts, their size-related effects on the immune system and allergic disease remain largely unknown. This knowledge gap is particularly concerning since metals are common inducers of allergic contact dermatitis, occupational asthma, and allergic adjuvancy.Overall, more scientific knowledge exists regarding the potential for metal-containing nanomaterials to exacerbate allergic disease than to their potential to induce allergic disease. Furthermore, effects of metal nanomaterial exposure on respiratory allergy have been more thoroughly-characterized than their potential influence on dermal allergy. Despite the existence of such knowledge, specific correlations between metal nanomaterial physico-chemical properties and their allergic effects have yet to be consistently demonstrated. As the number of emerging nanomaterials continues to increase, the delineation of these relationships has the potential to advance risk assessment efforts by helping to identify specific agents that may present an elevated risk for allergic effects.Two sets of studies were designed to begin addressing some of the knowledge gaps associated with the immunotoxic potential of metal nanomaterials in the context of allergic disease. The hypothesis of these studies was that nanomaterials comprised of metal constituents with known immunomodulatory potential augment, induce, and elicit allergic disease more readily than larger forms of the materials. Moreover, the nature of the immune responses caused by exposure to these metal nanomaterials, as well as the magnitude of the effects, correlates best with the parameter of dose surface area.The first set of studies incorporated NiO particles with different physico-chemical properties into an in vivo time course study and OVA asthma model. Results from the time course study demonstrated that the smaller NiO particles caused more pronounced pulmonary injury and inflammation, a discrepancy that could be mitigated by normalizing the administered particle dose to the surface area of the larger material. In the OVA model, augmentation of the allergic response was largely conserved with respect to NiO surface area, wherein larger doses caused polarization of pulmonary immune reposes towards a Th1/17-dominant state and smaller doses induced Th2-skewed responses. Despite this association, several immune markers, including total IgE production, BAL eosinophil number, and Penh response correlated better with other metrics, such as particle size.The second set of studies employed Au particles and nanoparticles to assess size-specific differences in allergenic potential. Neither particle was associated with potential for skin...

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Diagnosis of sentinel lymph nodes in patients with cancer of the larynx and laryngopharynx using a new radiopharmaceutical based on technetium-99m-labeled gamma aluminum oxide

et al. 2023

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Аim. To study the possibility of using a radiopharmaceutical based on aluminum oxide labeled with 99mТс ([99mТс]-Al2O3) for the diagnosis of sentinel lymph nodes (SLN) in tumors of the larynx and laryngopharynx in comparison with a phytate colloid ([99mTc]-phytate colloid).Materials and methods. The study included patients with cancer of the larynx and laryngopharynx (T2–4N0M0) (n = 54). In the prospective group (n = 30), [99mТс]-Al2O3 was used as a radiopharmaceutical, in the retrospective group (n = 24), [99mТс]-phytate colloid was used. All radiopharmaceuticals were introduced endoscopically into the submucosal space along the periphery of the tumor. After 18 hours, single-photon emission computed tomography (SPECT) and intraoperative SLN detection were performed.Results. In the retrospective group, SLNs were detected in 20 out of 24 patients. A total of 32 lymph nodes were identified in the retrospective group. The median number of detected lymph nodes in one patient was 1.3 [0–3], the intensity of the radiopharmaceutical uptake on scintigrams was 2.2 [0.7–8.1], intraoperatively – 4 [1.6–9.0]. In the prospective group, [99mTc]-Al2O3 uptake in the lymph nodes of the neck was determined in 27 patients (90%); in3 patients, SLNs were not visualized. A total of 57 lymph nodes were identified (in 27 patients). The median number of visualized SLNs was 1.5 [0–5], the intensity of [99mТс]-Al2O3 uptake according to SPECT and intraoperative detection was 4.8 [0.7–19.4] and 6 [1.1–22.0], respectively.Conclusion. The most significant advantage of using [99mТс]-Al2O3 as a radiopharmaceutical is its high uptake in SLNs, which leads to an increase in the sensitivity of the method as a whole up to 90 versus 83% when using [99mTc]-phytate colloid.

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Study of the Allergizing Properties of Nanocolloid,99mTc–Al2O3 Radiopharmaceutical in Experiment

Cited by 4 publications

References 1 publication

Untitled

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Metal Nanomaterials: Immune Effects and Implications of Physicochemical Properties on Sensitization, Elicitation, and Augmentation of Allergic Disease

Diagnosis of sentinel lymph nodes in patients with cancer of the larynx and laryngopharynx using a new radiopharmaceutical based on technetium-99m-labeled gamma aluminum oxide

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