Study of the composition of volatile organic compounds emitted by the filamentous fungus Fusarium culmorum by gas chromatography-mass spectrometry combined with solid phase microextraction

Savel’eva, E. I.; Gavrilova, Olga; Gagkaeva, Tatiana

doi:10.1134/s1070363214130180

Cited by 7 publications

(5 citation statements)

References 15 publications

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“…The RI s of phenanthrene and chrysene, reported in , are higher as compared to the respective values in the official NIST database . Note that corrected RI of trichodiene is 1533, which well agrees with published data . Thus, our estimates for the RI s of components eluted from the chromatographic column earlier than trichodiene can be considered as correct values, whereas those for later eluted components may involve certain errors.…”

Section: Resultssupporting

confidence: 89%

Study of the Vapor Phase Over Fusarium Fungi Cultured on Various Substrates

Savelieva

Gustyleva

Kessenikh

et al. 2016

Chemistry & Biodiversity

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The compositions of volatile organic compounds (VOCs) emitted by Fusarium fungi (F. langsethiae, F. sibiricum, F. poae, and F. sporotrichioides) grown on two nutritive substrates: potato sucrose agar (PSA) and autoclaved wheat kernels (WK) were investigated. The culturing of fungi and study of their VOC emissions were performed in chromatographic vials at room temperature (23 - 24 °C) and the VOCs were sampled by a solid-phase microextraction on a 85 μm carboxen/polydimethylsiloxane fiber. GC/MS was performed using a 60-m HP-5 capillary column. Components of the VOC mixture were identified by electron impact mass spectra and chromatographic retention indices (RIs). The most abundant components of the VOC mixture emitted by Fusarium fungi are EtOH, AcOH, (i) BuOH, 3-methylbutan-1-ol, 2-methylbutan-1-ol, ethyl 3-methylbutanoate, terpenes with M 136, sesquiterpenes with M 204 (a total of about 25), and trichodiene. It was found that the strains grown on PSA emit a wider spectrum and larger amount of VOCs compared with those grown on wheat kernels. F. langsethiae strain is the most active VOC producer on both substrates. The use of SPME and GC/MS also offers the potential for differentiation of fungal species and strains.

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Section: Resultssupporting

confidence: 89%

Study of the Vapor Phase Over Fusarium Fungi Cultured on Various Substrates

Savelieva

Gustyleva

Kessenikh

et al. 2016

Chemistry & Biodiversity

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“…In all growth conditions combined, we have detected the specific compounds known to be emitted by the two fungal species, validating our sampling method: germacrene A, (+)-sativene, and isoprene for C. sativus [30] and epi-bicyclosesquiphellandrene, βphellandrene [33], and p-xylene for F. culmorum [35].…”

Section: Discussionsupporting

confidence: 67%

“…a Chemical family b CAS number (Chemical Abstracts Service) c Compound known in the literature to be emitted by 1 = fungi, 2 = Fusarium sp., 3 = F. culmorum, 4 = Cochliobolus sp., 5 = C. sativus (https://bioinformatics.charite.de/mvoc/index.php, accessed on 2 May 2022,[33][34][35]) d Kovats indices calculated on a VF-Wax polar capillary column with a homologous series of n-alkanes (C7-C30) e…”

mentioning

confidence: 99%

Weapons against Themselves: Identification and Use of Quorum Sensing Volatile Molecules to Control Plant Pathogenic Fungi Growth

et al. 2022

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Quorum sensing (QS) is often defined as a mechanism of microbial communication that can regulate microbial behaviors in accordance with population density. Much is known about QS mechanisms in bacteria, but fungal QS research is still in its infancy. In this study, the molecules constituting the volatolomes of the plant pathogenic fungi Fusarium culmorum and Cochliobolus sativus have been identified during culture conditions involving low and high spore concentrations, with the high concentration imitating overpopulation conditions (for QS stimulation). We determined that volatolomes emitted by these species in conditions of overpopulation have a negative impact on their mycelial growth, with some of the emitted molecules possibly acting as QSM. Candidate VOCs related to QS have then been identified by testing the effect of individual volatile organic compounds (VOCs) on mycelial growth of their emitting species. The antifungal effect observed for the volatolome of F. culmorum in the overpopulation condition could be attributed to ethyl acetate, 2-methylpropan-1-ol, 3-methylbutyl ethanoate, 3-methylbutan-1-ol, and pentan-1-ol, while it could be attributed to longifolene, 3-methylbutan-1-ol, 2-methylpropan-1-ol, and ethyl acetate for C. sativus in the overpopulation condition. This work could pave the way to a sustainable alternative to chemical fungicides.

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“…Solid-phase extraction (SPE) is one of the main used methods for extraction. In the SPE method, analytes are adsorbed and prevented from disintegrating and are stored for a certain time without any variation in its concentration or nature [8][9][10][11]47]. The SPE method can extract a wide range of organic compounds from food, environmental, and biological samples: wastewater, pharmaceuticals [12,13], personal care products [14], and pesticides [15].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of chitosan functionalized magnetic carbon nanotubes for dispersive solid‐phase extraction of bromocresol green

Abniki

Moghimi

2021

Micro & Nano Letters

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This study presents a new and rapid method for preconcentration, measurement, and removal of trace amounts of coloured wastewater using chitosan functionalized magnetic carbon nanotubes in aqueous samples. This method involves a two-phase system in which the donor phase contains an aqueous phase with bromocresol green, and the acceptor phase contains functionalized magnetic carbon nanotubes with chitosan. Experiments were carried out in two stages of extraction of bromocresol green from aqueous solution and desorption of bromocresol green using ethanol solvent. The samples were analysed by UV-Vis spectrophotometer. Extraction parameters were investigated such as the effect of organic solvent, pH of the donor and acceptor phases, extraction time, interference species, and volume of elution solvent. The isotherm of bromocresol green adsorption was fine described by the Langmuir model, and the adsorption capacity in the maximum was 28.49 mg g −1 . The preconcentration factor and limit of detection for bromocresol green were 14.3 and 7.5 μg L −1 , respectively. A linear range is observed between 0.1 and 20 mg L −1 . Finally, the chitosan functionalized magnetic carbon nanotubes (Fe 3 O 4 @MWCNT-CN) exhibited a high potential for removing the bromocresol green from aqueous samples.This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

show abstract

Study of the composition of volatile organic compounds emitted by the filamentous fungus Fusarium culmorum by gas chromatography-mass spectrometry combined with solid phase microextraction

Cited by 7 publications

References 15 publications

Study of the Vapor Phase Over Fusarium Fungi Cultured on Various Substrates

Study of the Vapor Phase Over Fusarium Fungi Cultured on Various Substrates

Weapons against Themselves: Identification and Use of Quorum Sensing Volatile Molecules to Control Plant Pathogenic Fungi Growth

Synthesis of chitosan functionalized magnetic carbon nanotubes for dispersive solid‐phase extraction of bromocresol green

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