Study of the cortical representation of whisker directional deflection using voltage-sensitive dye optical imaging

Tsytsarev, Vassiliy; Pope, Daniel L. W.; Pumbo, Elena; Yablonskii, A. L.; Hofmann, Michaël

doi:10.1016/j.neuroimage.2010.06.022

Cited by 32 publications

(33 citation statements)

References 54 publications

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“…Thus, based on the data analysis from these two barrels, our results could be consistent with the presence of an intrabarrel somatotopic directional map (Andermann and Moore 2006;Kerr et al 2007;Kremer et al 2011). However, the results from two barrels with exceptional signal-to-noise ratio are far from conclusive and further studies are needed (Tsytsarev et al 2010a(Tsytsarev et al , 2010b.…”

Section: Discussionsupporting

confidence: 80%

Voltage-sensitive dye imaging reveals shifting spatiotemporal spread of whisker-induced activity in rat barrel cortex

Lustig

Friedman

Winberry

et al. 2013

Journal of Neurophysiology

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Lustig BR, Friedman RM, Winberry JE, Ebner FF, Roe AW. Voltage-sensitive dye imaging reveals shifting spatiotemporal spread of whiskerinduced activity in rat barrel cortex. J Neurophysiol 109: 2382-2392, 2013. First published February 6, 2013 doi:10.1152/jn.00430.2012.-In rats, navigating through an environment requires continuous information about objects near the head. Sensory information such as object location and surface texture are encoded by spike firing patterns of single neurons within rat barrel cortex. Although there are many studies using singleunit electrophysiology, much less is known regarding the spatiotemporal pattern of activity of populations of neurons in barrel cortex in response to whisker stimulation. To examine cortical response at the population level, we used voltage-sensitive dye (VSD) imaging to examine ensemble spatiotemporal dynamics of barrel cortex in response to stimulation of single or two adjacent whiskers in urethaneanesthetized rats. Single whisker stimulation produced a poststimulus fluorescence response peak within 12-16 ms in the barrel corresponding to the stimulated whisker (principal whisker). This fluorescence subsequently propagated throughout the barrel field, spreading anisotropically preferentially along a barrel row. After paired whisker stimulation, the VSD signal showed sublinear summation (less than the sum of 2 single whisker stimulations), consistent with previous electrophysiological and imaging studies. Surprisingly, we observed a spatial shift in the center of activation occurring over a 10-to 20-ms period with shift magnitudes of 1-2 barrels. This shift occurred predominantly in the posteromedial direction within the barrel field. Our data thus reveal previously unreported spatiotemporal patterns of barrel cortex activation. We suggest that this nontopographical shift is consistent with known functional and anatomic asymmetries in barrel cortex and that it may provide an important insight for understanding barrel field activation during whisking behavior. whisker function; optical imaging; somatosensory; barrel cortex; VSD IN RODENTS, THE TOPOGRAPHICAL single whisker-to-single cortical barrel relationship has long been recognized as a principle of cortical functional organization (Woolsey and Van der Loos 1970). Despite this clear one-to-one relationship, neurophysiological studies have revealed that principal whiskers are also modulated by subthreshold influences from distant sites (Brecht and

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Section: Discussionsupporting

confidence: 80%

Voltage-sensitive dye imaging reveals shifting spatiotemporal spread of whisker-induced activity in rat barrel cortex

Lustig

Friedman

Winberry

et al. 2013

Journal of Neurophysiology

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“…The voltage-sensitive dye RH-1691 (Optical Imaging; 1.0 mg/ml in ACSF) was applied to the exposed dural surface for 45 min. After staining, the cortex was washed with dye-free ACSF for 15 min (Grinvald and Hildesheim 2004;Tsytsarev et al 2009Tsytsarev et al , 2010. The cortical surface was covered with high-density silicone oil and then sealed with a 0.1-mm-thick cover glass to suppress the brain pulsation originating from cardiovascular and respiratory movements.…”

Section: Methodsmentioning

confidence: 99%

“…For data analysis, we constructed a pseudocolor map using firstframe analysis and then averaging the data for each session (Ferezou et al 2006;Orbach et al 1985;Petersen et al 2003;Shoham et al 1999;Tsytsarev et al 2009Tsytsarev et al , 2010). Thus we obtained pseudocolor maps of the areas activated by whisker stimulus.…”

Section: Methodsmentioning

confidence: 99%

“…We performed comparative analysis of the areas activated by whisker stimulation. The sizes of the activated areas were calculated and averaged after setting the threshold at half of the maximum signal strength (Tsytsarev et al 2009(Tsytsarev et al , 2010. At maximal size, the activity field covered an area of 2,972 pixels (1.86 mm 2 ) in CxNR1KO and 1,137 pixels (0.71 mm 2 ) in control animals, and the confidences were 591 (0.37 mm 2 ) and 202 (0.13 mm 2 ), respectively (Fig.…”

Section: Reduction Of Tonic Responses To Whisker Deflection Of Barrelmentioning

confidence: 99%

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Functional significance of cortical NMDA receptors in somatosensory information processing

Akkentli

Tsytsarev

et al. 2013

Journal of Neurophysiology

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“…Increasing the penetration depth would broaden the application of FLOT for neuroscience research. Since the optical properties of the living mouse brain are too complicated to mimic using a simple phantom, a 150-μm glass capillary filled with voltage-sensitive dye (commonly used for reporting neural activities [6, [32][33][34][35]) was inserted into a mouse brain in vivo to demonstrate the feasibility of HDR-FLOT to increase penetration depth.…”

Section: Experimental Designmentioning

confidence: 99%

High-dynamic-range fluorescence laminar optical tomography (HDR-FLOT)

Tang¹,

Liu²,

Tsytsarev³

et al. 2017

Biomed. Opt. Express

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Three-dimensional fluorescence laminar optical tomography (FLOT) can achieve resolutions of 100-200 µm and penetration depths of 2-3 mm. FLOT has been used in tissue engineering, neuroscience, as well as oncology. The limited dynamic range of the chargecoupled device-based system makes it difficult to image fluorescent samples with a large concentration difference, limits its penetration depth, and diminishes the quantitative accuracy of 3D reconstruction data. Here, incorporating the high-dynamic-range (HDR) method widely used in digital cameras, we present HDR-FLOT, increasing penetration depth and improving the ability to image fluorescent samples with a large concentration difference. The method was tested using an agar phantom and a B6 mouse for brain imaging in vivo.

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Study of the cortical representation of whisker directional deflection using voltage-sensitive dye optical imaging

Cited by 32 publications

References 54 publications

Voltage-sensitive dye imaging reveals shifting spatiotemporal spread of whisker-induced activity in rat barrel cortex

Voltage-sensitive dye imaging reveals shifting spatiotemporal spread of whisker-induced activity in rat barrel cortex

Functional significance of cortical NMDA receptors in somatosensory information processing

High-dynamic-range fluorescence laminar optical tomography (HDR-FLOT)

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