Study of the Distribution of Guanidine Polymethacrylate to Create a New Generation of Nanocontainers That Provide Targeted Drug Delivery to Target Cells

Abstract

Investigation of the distribution of guanidine polymethacrylate - as the basis for a future nanocontainer for targeted transport of drugs in living organs and tissues and assessment of its effect on the body as a whole. To assess the effect on the body and the distribution of PMAG in tissues and organs, we used 24 experimental healthy laboratory rats: identical in series, sex, weight and age. A solution of PMAG in 0.9% sodium chloride in three concentrations: 2.5 mg / ml, 5 mg / ml, 10 mg / ml was administered to rats intravenously. Pieces of organs and tissues were fixed in 10% neutral formalin and embedded in paraffin according to the generally accepted technique. Histological sections, 5-7 μm thick, were stained with hematoxylin and eosin. The preparations were examined under an OLYMPUS BX 43 microscope with a digital video camera. Intramuscular injection of nanomaterial causes structural changes in the intestinal wall of rats, which are manifested after the introduction of 1 ml of solution at a dosage of 2.5 mg / ml after two hours. Changes are manifested in circulatory disorders, leukocyte infiltration, degenerative changes and epithelial necrosis. Alterative changes increase as the concentration of the injected solution and the exposure time increase. The results obtained indicate that at this stage it is not yet possible to immediately use this material for targeted drug delivery, but further developments in this direction seem quite promising for the development of new therapeutic anticancer drugs, an innovative strategy for personalized molecular therapy for intestinal cancer.