Study of the dopamine effect into cell solutions by impedance analysis

Paivana, Georgia; Apostolou, Theofylaktos; Kaltsas, G.; Kintzios, Spyridon

doi:10.1088/1742-6596/931/1/012010

Cited by 3 publications

(2 citation statements)

References 16 publications

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“…In [2], a normal electric eld was applied to the surface of a N2a culture, the maximum length of differentiated neurites (90µm) was obtained at the optimal electric eld amplitude of 250mV/mm. Studies with N2a also characterize the response to various doses of dopamine, using electrical impedance spectroscopy (EIS) [31]. For osteoblasts, we have also found works that prove the functional improvement of long-term behavior when subjected to biphasic electrical pulses [32], using commercial electrical stimulators on the surfaces of titanium nanotubes.…”

Section: Introductionmentioning

confidence: 77%

DC Electrical Stimulation effects on N2a and MC3T3 cell lines

Martín

Bocio

Scagliusi

et al. 2022

Preprint

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Background: Electrical stimulation is a novel tool to promote the differentiation and proliferation of precursor cells. In this work we have studied the effects of direct current (DC) electrical stimulation on neuroblastoma (N2a) and osteoblast (MC3T3) cell lines as a model for nervous and bone tissue regeneration, respectively. We have developed the electronics and encapsulation of a proposed stimulation system and designed a setup and protocol to stimulate cell cultures. Methods: Cell cultures were subjected to several assays to assess the effects of electrical stimulation on them. N2a cells were analyzed using microscope images and an inmunofluorescence assay, differentiated cells were counted and neurites were measured. MC3T3 cells were subjected to an AlamarBlue assay for viability, ALP activity was measured, and a real time PCR was carried out.Results: Our results show that electrically stimulated cells had more tendency to differentiate in both cell lines when compared to non-stimulated cultures, paired with a promotion of neurite growth and polarization in N2a cells and an increase in proliferation in MC3T3 cell line.Conclusions: These results prove the effectiveness of electrical stimulation as a tool for tissue engineering and regenerative medicine, both for neural and bone injuries. Bone progenitor cells submitted to electrical stimulation have a higher tendency to differentiate and proliferate, filling the gaps present in injuries. On the other hand, neuronal progenitor cells differentiate, and their neurites can be polarized to follow the electric field applied.

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Section: Introductionmentioning

confidence: 77%

DC Electrical Stimulation effects on N2a and MC3T3 cell lines

Martín

Bocio

Scagliusi

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…In [2], a normal electric field was applied to the surface of a N2a culture, the maximum length of differentiated neurites (90 μm) was obtained at the optimal electric field amplitude of 250 mV/mm. Studies with N2a also characterize the response to various doses of dopamine, using electrical impedance spectroscopy (EIS) [31].…”

Section: Introductionmentioning

confidence: 99%

DC electrical stimulation enhances proliferation and differentiation on N2a and MC3T3 cell lines

et al. 2022

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Background Electrical stimulation is a novel tool to promote the differentiation and proliferation of precursor cells. In this work we have studied the effects of direct current (DC) electrical stimulation on neuroblastoma (N2a) and osteoblast (MC3T3) cell lines as a model for nervous and bone tissue regeneration, respectively. We have developed the electronics and encapsulation of a proposed stimulation system and designed a setup and protocol to stimulate cell cultures. Methods Cell cultures were subjected to several assays to assess the effects of electrical stimulation on them. N2a cells were analyzed using microscope images and an inmunofluorescence assay, differentiated cells were counted and neurites were measured. MC3T3 cells were subjected to an AlamarBlue assay for viability, ALP activity was measured, and a real time PCR was carried out. Results Our results show that electrically stimulated cells had more tendency to differentiate in both cell lines when compared to non-stimulated cultures, paired with a promotion of neurite growth and polarization in N2a cells and an increase in proliferation in MC3T3 cell line. Conclusions These results prove the effectiveness of electrical stimulation as a tool for tissue engineering and regenerative medicine, both for neural and bone injuries. Bone progenitor cells submitted to electrical stimulation have a higher tendency to differentiate and proliferate, filling the gaps present in injuries. On the other hand, neuronal progenitor cells differentiate, and their neurites can be polarized to follow the electric field applied.

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Bioelectrical Analysis of Various Cancer Cell Types Immobilized in 3D Matrix and Cultured in 3D-Printed Well

et al. 2019

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Cancer cell lines are important tools for anticancer drug research and assessment. Impedance measurements can provide valuable information about cell viability in real time. This work presents the proof-of-concept development of a bioelectrical, impedance-based analysis technique applied to four adherent mammalian cancer cells lines immobilized in a three-dimensional (3D) calcium alginate hydrogel matrix, thus mimicking in vivo tissue conditions. Cells were treated with cytostatic agent5-fluoruracil (5-FU). The cell lines used in this study were SK-N-SH, HEK293, HeLa, and MCF-7. For each cell culture, three cell population densities were chosen (50,000, 100,000, and 200,000 cells/100 μL). The aim of this study was the extraction of mean impedance values at various frequencies for the assessment of the different behavior of various cancer cells when 5-FU was applied. For comparison purposes, impedance measurements were implemented on untreated immobilized cell lines. The results demonstrated not only the dependence of each cell line impedance value on the frequency, but also the relation of the impedance level to the cell population density for every individual cell line. By establishing a cell line-specific bioelectrical behavior, it is possible to obtain a unique fingerprint for each cancer cell line reaction to a selected anticancer agent.

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Study of the dopamine effect into cell solutions by impedance analysis

Cited by 3 publications

References 16 publications

DC Electrical Stimulation effects on N2a and MC3T3 cell lines

DC Electrical Stimulation effects on N2a and MC3T3 cell lines

DC electrical stimulation enhances proliferation and differentiation on N2a and MC3T3 cell lines

Bioelectrical Analysis of Various Cancer Cell Types Immobilized in 3D Matrix and Cultured in 3D-Printed Well

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