Study of the early growth and branching of Streptomyces tendae by means of an image processing system

Reichl, Udo; Buschulte, T. K.; Gilles, Ernst Dieter

doi:10.1111/j.1365-2818.1990.tb02976.x

Cited by 38 publications

(20 citation statements)

References 12 publications

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“…When its length reaches y MA , it comes into the category of a small hypha and grows linearly because the length of the apical compartments that are responsible for hyphal tip extension in these hyphae are constant. The assumption that a hypha first grows exponentially and then grows linearly has been verified by several experimental observations (Trinci & Saunders 1977;Reichl et al 1990), and it is also theoretically rational if only compartment A is responsible for hyphal tip extension. In a small hypha, the length of the apical compartment is constant, and extension of compartment A leads to the net formation of compartment B, i.e.…”

Section: Morphologically Structured Modelmentioning

confidence: 92%

A Population-Based Morphologically Structured Model for Hyphal Growth and Product Formation in Streptomycin Fermentation

Liu

Xing

Han

2005

World J Microbiol Biotechnol

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A population model discriminating the hyphae according to the hyphal length and a morphologically structured model considering the specific function of different morphological forms of a hypha are combined together to describe mycelial growth, substrate consumption and secondary metabolite formation in streptomycin fermentation. In the population model, the growth modes of hyphae with different age or length are considered, while in the morphologically structured model, the morphological forms of hyphae and their functions in growth and metabolism are described. The population model and the morphologically structured model are interrelated by a branching function and a differentiation function. In the model, the growth rate of immature apical compartment is distinguished from those of matured ones, branching is proposed to occur only in the subapical region, and the hyphal compartment is assumed to synthesize secondary metabolites. The model is successfully applied to simulate the batch fermentation process of streptomycin production. The growth characteristics of filamentous microorganisms are also discussed using the model predictions.

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Section: Morphologically Structured Modelmentioning

confidence: 92%

A Population-Based Morphologically Structured Model for Hyphal Growth and Product Formation in Streptomycin Fermentation

Liu

Xing

Han

2005

World J Microbiol Biotechnol

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“…When a quantitative analysis of mycelial trees is needed, a digitising table [82] or an image processing system can be used [83,118,128]. Reichl et al [129] have developed an image analysis for the characterisation of pellets during submerged growth of Streptomyces tendae.…”

Section: Methods Of Measuring the Morphologymentioning

confidence: 99%

“…Photomicroscopic measurement or image analysis of individual mycelia has been employed by several workers to determine the effects of mycelial morphology upon broth rheology and productivity [46,82,83,118]. In particular, the automated image analysis method of Packer and Thomas [83] provides fast and accurate measurement of morphology of freely dispersed mycelia.…”

Section: Mycelial Pellets Formationmentioning

confidence: 99%

Understanding the morphology of fungi

Pazouki

Panda

2000

Bioprocess Engineering

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Filamentous fungi comprise an industrially very important collection of microorganisms, since they are used for the production of a wide variety of products ranging from primary metabolites to secondary metabolites and further on to industrial enzymes (such as proteases, lipases and antibiotics). It is known that fungal morphology is often considered as one of the key parameters in industrial production. For the production of fungal metabolite products, the desired morphology varies from one product to another. Many parameters affect the morphology of fungi during the process of fermentation, among them speed of agitation, speci®c growth rate, dissolved oxygen, number of spores or conidia per liter of fermentation broth are important and should be considered when higher yield is desired in the process. It is, therefore, of considerable importance to understand the mechanism underlying the morphology of the cell, its growth and product formation by ®lamentous fungi. Such knowledge may be used in the optimization of the microbial process. Several literatures with various fungi to study their morphology, relating enzyme or product production to the character of the fungi in the study is reviewed. It is also considered that how the process parameters affects the morphology. The aim of this communication is to review the relevant literature to understand the morphology of ®lamentous fungi. List of symbols bhyphal density, lm/tip D i impeller diameter, m D pellet diameter (in Eqs. 4 and 5), mm D r dilution rate, h )1 k constant, number of section used for branching (in Eq. 7) K c constant K r constant (in Eq. 5) K r linear colony radial growth rate (in Eq. 1), lm/h L e mean length of main hyphae, lm L e *mean dimensionless length, lm L t mean total length of hyphae, lm L hgu mean length of hyphal growth unit, lm M 0 dry weight of mycelia at time t 0 , g M 1 dry weight of mycelia at time t 1 , g n number of disrupted pellets N impeller speed, h )

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“…Several workers have developed image analysis methods to study mycelia of this form. In early work, Reichl et al (1990) used image analysis to examine Streptomyces tendae growing upon cellophane on top of nutritionally complete solid media. Although effective in measuring growth, the method was restricted to examination of small numbers of hyphae.…”

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confidence: 99%