Study of the Effect of Material of Microfluid Chip on the Polymerase Chain Reaction

Microfluidic delivery of cutting enzymes for fragmentation of surface-adsorbed DNA molecules

“…Flow rates [ 47 ] and enzyme reaction rates (both promotion and inhibition [ 48 ]) have been shown to be influenced as well as concentrations of analytes in assays (affecting their accuracy and sensitivity [ 49 ]). A number of surface modification strategies have been developed, including: 1) surface coatings, by adsorption, either statically (by pre-coating [ 42 , 46 , 50 , 51 ]) or dynamically (added to reaction mixtures [ 51 – 53 ]), 2) grafting of molecules to [ 54 ] or from [ 55 ] the surfaces, 3) addition of surface-active polymers to the curing elastomer mix [ 41 , 43 , 44 , 56 ] and 4) UV/ozone [ 57 ] and oxygen plasma [ 58 , 59 ] treatments. Also, solvent soaks [ 60 , 61 ] or high-temperature post-curing outgassing [ 62 , 63 ] have been used to remove un-crosslinked oligomers and small molecules from the cured elastomers.…”

“…Also, solvent soaks [ 60 , 61 ] or high-temperature post-curing outgassing [ 62 , 63 ] have been used to remove un-crosslinked oligomers and small molecules from the cured elastomers. These treatments have been shown to be effective in reducing protein [ 41 , 43 , 44 ] and fluorescent probe [ 42 , 45 , 49 ] adsorption and to improve PCR yields [ 41 , 46 , 50 – 53 ] Numerous reviews of surface treatments have appeared, among them references [ 52 , 64 – 68 ]. Due to the wide variety of elastomer formulations, temperature ranges, reactants and geometric considerations (such as surface-to-volume ratios) encountered in applications, precise comparisons with previous work are difficult and tests for any new experimental conditions should be made to determine efficacy.…”

“…Also, solvent soaks [60,61] or high-temperature post-curing outgassing [62,63] have been used to remove uncrosslinked oligomers and small molecules from the cured elastomers. These treatments have been shown to be effective in reducing protein [41,43,44] and fluorescent probe [42,45,49] adsorption and to improve PCR yields [41,46,[50][51][52][53] Numerous reviews of surface treatments have appeared, among them references [52,[64][65][66][67][68]. Due to the wide variety of elastomer…”