Study of the Gastroprotective Effect of Extracts and Semipurified Fractions ofChresta martiiDC. and Identification of Its Principal Compounds

Abstract

Chresta martii (Asteraceae) is a species widely used by the population of the Xingu region of Sergipe, Brazil, in the form of a decoction (aerial parts) for the treatment of gastrointestinal diseases. The study aims to assess the gastroprotective activity of organic extracts and semipurified fractions and identify the principal compounds present in C. martii responsible for such activity. The organic extracts (cyclohexane: ECCm, ethyl acetate: EACm, and ethanol: EECm) were obtained from the dried aerial parts (500 g) of C. martii. For evaluation of the gastroprotective activity of extracts (50, 100, or 200 mg/kg; p.o.), male Swiss Webster mice (25–30 g) were used which had gastric ulcers induced by indomethacin (40 mg/kg, s.c.) or ethanol (0.2 mL/animal; p.o.). Among the extracts evaluated, EACm exhibited significant (P < 0.05) gastroprotective activity in the models used. The fractionation of EACm was performed in a silica gel column 60 eluted with the following compounds: [chloroform—F1 yield (10%)], [chloroform/ethyl acetate (1/1)—F2 yield (6%)], [ethyl acetate—F3 yield (8%)], and [ethyl/methanol acetate (1/1)—F4 yield (5%)]. Of the fractions described above, the F1 (25 mg/kg; p.o.) had greater gastroprotective activity (P < 0.05) than that displayed by ranitidine (80 mg/kg; p.o.) in the ethanol-induced ulcer model. The refractionation of F1 produced 23 subfractions and from these two yellow amorphous compounds were obtained by recrystallization, Rf: 0.46 and 0.31 (ethyl acetate : chloroform 5 : 5). The compounds isolated were characterized by nuclear magnetic resonance spectroscopy (1H-NMR and 13C-NMR) and identified as flavones: chrysoeriol (yield: 0.43%) and 3′,4′-dimethoxyluteolin (yield: 0.58%). Conclusion. Flavone 3′,4′-dimethoxyluteolin is the principal compound present in the species C. martii and is probably responsible for gastroprotective activity observed in this species.

NF-κB and Angiogenesis Inhibitors from the Aerial Parts of Chresta martii

“…The UV-PDA spectra of 1 – 8 and 11 suggested the presence of sesquiterpene lactones, and those of 9 , 10 , and 12 – 14 were characteristic for flavonoids (Figure ). Two flavonoids recently isolated from this plant were dereplicated as chrysoeriol ( 12 , t R 38.5 min, m / z 299.0562 [M – H] − ), a known NF-κB inhibitor previously isolated from Brucea javanica , and luteolin 3′,4′-dimethyl ether ( 14 , t R 42.5 min, m / z 315.0878 [M + H] + ) . The identity of 14 was confirmed by comparison of the retention time with a standard.…”

“…This activity was found to be mediated by alpha-2 adrenoceptor activation, but not by nitric oxide release, opioid receptor activation, or prostaglandin synthesis . Recently, the flavonoids chrysoeriol and 3′,4′-dimethoxyluteolin were reported from this plant …”

Protective effect of Chresta martii extract on the zymosan-induced temporomandibular joint arthritis in rats

“…[18][19][20][21][22] Our group has been investigating the pharmacological properties of this species since 2012 and its mechanisms of action have been gradually elucidated. 26,27 Given the above, we decided to assess whether CEE would improve inflammatory pain.…”