This study explores an approach to detect virus-laden droplets in the ambient air. An air-coupled photoacoustic (PA) technique is investigated for this purpose. The free space PA system is developed using an air-coupled transducer with a center frequency of 350 kHz and a nanosecond pulsed laser operating at wavelength 533 nm. Water droplets containing 80 nm Au nanoparticles were aerosolized using a custom-built spraying system. The size of the droplets generated was in the range of a few hundred nanometers to 100 µm. Au nanoparticles of four concentrations (0, 8x10 -12 , 16x10 -12 , and 32x10 -12 mol/L) were sprayed through the laser, where the average PA signal from the droplets was 3.11±2.35, 1.28±1.26, 0.99±0.97, and 0.92±1.11 mV/mJ, respectively. The study showed, paradoxically, that water droplets without Au nanoparticles had a higher PA signal than droplets containing Au nanoparticles. A numerical analysis using a finite difference time domain method was used to explore the reasons for this unexpected finding. Results demonstrated that the droplets could potentially focus the light, significantly increasing the fluence at the focus. When Au nanoparticles were present, the fluence within the droplet decreased, resulting in a lower PA signal.