Study of the microbial diversity of a panel of Belgian artisanal cheeses associated with challenge studies for Listeria monocytogenes

Gérard, Amaury; El-Hajjaji, Soundous; Burteau, Sophie; Fall, Papa Abdoulaye; Pirard, Barbara; Taminiau, Bernard; Daube, Georges; Sindic, Marianne

doi:10.1016/j.fm.2021.103861

Cited by 20 publications

(12 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Extracts were stored at −20 °C until used. Libraries and sequencing were performed as previously described [ 27 ]. Briefly, the amplification of V1-V3 regions of the 16S rRNA bacterial gene was performed using the primers 5′-GAGAGTTTGATYMTGGCTCAG-3′ forward and 5′-ACCGCGGCTGCTGGCAC-3′reverse with overhand adapters.…”

Section: Methodsmentioning

confidence: 99%

Membrane Vesicles of Toxigenic Clostridioides difficile Affect the Metabolism of Liver HepG2 Cells

et al. 2023

Self Cite

View full text Add to dashboard Cite

Clostridioides difficile infection (CDI) appears to be associated with different liver diseases. C. difficile secretes membrane vesicles (MVs), which may be involved in the development of nonalcoholic fatty liver disease (NALFD) and drug-induced liver injury (DILI). In this study, we investigated the presence of C. difficile-derived MVs in patients with and without CDI, and analyzed their effects on pathways related to NAFLD and DILI in HepG2 cells. Fecal extracellular vesicles from CDI patients showed an increase of Clostridioides MVs. C. difficile-derived MVs that were internalized by HepG2 cells. Toxigenic C. difficile-derived MVs decreased mitochondrial membrane potential and increased intracellular ROS compared to non-toxigenic C. difficile-derived MVs. In addition, toxigenic C. difficile-derived MVs upregulated the expression of genes related to mitochondrial fission (FIS1 and DRP1), antioxidant status (GPX1), apoptosis (CASP3), glycolysis (HK2, PDK1, LDHA and PKM2) and β-oxidation (CPT1A), as well as anti- and pro-inflammatory genes (IL-6 and IL-10). However, non-toxigenic C. difficile-derived MVs did not produce changes in the expression of these genes, except for CPT1A, which was also increased. In conclusion, the metabolic and mitochondrial changes produced by MVs obtained from toxigenic C. difficile present in CDI feces are common pathophysiological features observed in the NAFLD spectrum and DILI.

show abstract

Section: Methodsmentioning

confidence: 99%

Membrane Vesicles of Toxigenic Clostridioides difficile Affect the Metabolism of Liver HepG2 Cells

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…Data processing was performed using, respectively, the MOTHUR v1.44 package and the VSearch algorithm [ 22 ] for alignment, clustering and chimer detection as previously described by Gérard et al [ 23 ]. After cleaning process, sequences were clustered into operational taxonomic units (OTUs) at 97% of identity.…”

Section: Methodsmentioning

confidence: 99%

Chicory: Understanding the Effects and Effectors of This Functional Food

et al. 2022

Self Cite

View full text Add to dashboard Cite

Industrial chicory has been the subject of numerous studies, most of which provide clinical observations on its health effects. Whether it is the roasted root, the flour obtained from the roots or the different classes of molecules that enter into the composition of this plant, understanding the molecular mechanisms of action on the human organism remains incomplete. In this study, we were interested in three molecules or classes of molecules present in chicory root: fructose, chlorogenic acids, and sesquiterpene lactones. We conducted experiments on the murine model and performed a nutrigenomic analysis, a metabolic hormone assay and a gut microbiota analysis, associated with in vitro observations for different responses. We have highlighted a large number of effects of all these classes of molecules that suggest a pro-apoptotic activity, an anti-inflammatory, antimicrobial, antioxidant, hypolipidemic and hypoglycemic effect and also an important role in appetite regulation. A significant prebiotic activity was also identified. Fructose seems to be the most involved in these activities, contributing to approximately 83% of recorded responses, but the other classes of tested molecules have shown a specific role for these different effects, with an estimated contribution of 23–24%.

show abstract

“…Growth assessment studies, so-called challenge tests, may be carried out in order to investigate the ability of appropriately inoculated microorganisms of concern to grow or survive in the product under different reasonably foreseeable storage conditions and thus, meet the regulation's requirement. FBOs, based on the assessment of results of a challenge test, [21][22][23][24][25][26][27] must adopt the most effective strategies within their production processes and for the subsequent storage of food during the marketing phase in order to produce safe products for consumers [28,29].…”

Section: Introductionmentioning

confidence: 99%

“…In 2021, the Commission has published the "EURL Lm Technical Guidance Document on challenge tests and durability studies for assessing shelf life of ready-to-eat foods related to Lm" [30]. Several studies have been conducted and published regarding challenge testing and monitoring of Lm growth in many different RTE foods [24,31]. As mentioned in a review paper by Gerard et al [32], soft cheeses seem to pose a significant risk to consumers, because the pH and a w of soft cheeses are favorable for the reproduction of Lm.…”

Section: Introductionmentioning

confidence: 99%

Challenge Test for Assessing the Growth Potential of Listeria Monocytogenes in Greek Soft Cheese (Anthotyros)

et al. 2022

View full text Add to dashboard Cite

Foodborne listeriosis is one of the most serious and severe foodborne diseases, with a high mortality rate worldwide. Listeria monocytogenes’ (Lm) ability to survive under a diversity of conditions makes it a threat for food safety. Soft and semisoft cheeses are common RTE foods that support survival and growth of Lm due to their high moisture content and favorable pH. The aim of the present study was to assess, after artificial inoculation, the growth potential (Δ) of Lm in vacuum packed RTE soft Greek Anthotyros cheese. Growth potential of Lm was determined according to the new version of “EURL Lm Technical Guidance Document on challenge tests and durability studies for assessing shelf life of ready-to-eat foods related to Lm”. The results of our study revealed that the growth potential of Lm was (Δ) = 4.93 log10 CFU/g, indicating that the specific soft cheese “anthotyros” is a “Ready to eat food able to support growth of Lm and classified in food safety category 1.2 in the (EC) Regulation 2073/2005”. That means that the food business operator (FBO) must comply with the criteria that define that the bacterium must not be detected in 25 g (n = 5, c = 0) at the time of leaving the production plant. Through this study, we try to highlight the need for FBO to conduct relevant research to ensure that the ready-to-eat products which they produce, during their shelf life, do not support the growth of Lm.

show abstract

Study of the microbial diversity of a panel of Belgian artisanal cheeses associated with challenge studies for Listeria monocytogenes

Cited by 20 publications

References 49 publications

Membrane Vesicles of Toxigenic Clostridioides difficile Affect the Metabolism of Liver HepG2 Cells

Membrane Vesicles of Toxigenic Clostridioides difficile Affect the Metabolism of Liver HepG2 Cells

Chicory: Understanding the Effects and Effectors of This Functional Food

Challenge Test for Assessing the Growth Potential of Listeria Monocytogenes in Greek Soft Cheese (Anthotyros)

Contact Info

Product

Resources

About