Study of the new cross reactive monoclonal antibody against Neisseria meningitidis using flow cytometry analysis

Oliveira, L.M.C. De; Fazioli, Raquel A.; Gaspari, Elizabeth De

doi:10.1016/s0165-2478(97)86164-0

Cited by 2 publications

(3 citation statements)

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“…Considering the high incidence of meningococcal disease due to certain group B strains in Brazil, the studies of surface structures of class 5 proteins are important. Groups in different Brazilian laboratories have emphasized the importance of class 5 proteins [34,41,42] in Brazil. However, no analysis of the prevalence of class 5 types expressed in serogroup B strains in Brazil has been performed, despite the importance of such an analysis for the identification of prevalent class 5 proteins in our strains.…”

Section: Discussionmentioning

confidence: 99%

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Expression of class 5 antigens by meningococcal strains obtained from patients in Brazil and evaluation of two new monoclonal antibodies

E¹,

Zollinger²

2001

Braz J Infect Dis

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Determining the profile of antigen expression among meningococci is important for epidemiologic surveillance and vaccine development. To this end, two new mouse monoclonal antibodies (MAbs) have been derived against Neisseria meningitidis proteins (class 5). The MAbs were reactive against outer membrane antigens and were bactericidal. Selected anti-class 5 MAbs [(5.1)-3E6-2; (5.3)-3BH4-C7; (5.4)-1BG11-C7; (5.5)-3DH-F5G9 also 5F1F4-T3(5.c)], and the two new monoclonal antibodies C14F10Br2 (5.8) and 7F11B5Br3 (5.9), were then tested against different meningococcal strains, (63 strains of serogroup A, 60 strains of serogroup C (from 1972 to 1974); and 136 strains of serogroup B (from 1992) meningococci). Our results demonstrated that the expression of class 5 proteins in the N. meningitidis B Brazilian strains studied is highly heterogeneous. The serotypes and subtypes of B:4:P1.15, B:4:P1.9, B:4:P1.7, B:4:P1.3, B:4:P1.14, B:4:P1.16, B:4:NT, and B:NT:NT were detected in N. meningitidis B serogroups. The strains C:2a:P1.2 and A:4.21:P1.9 were dominant in the C and A serogroups, respectively. Serogroup B organisms expressed the class 5 epitopes 5.4 (18%), 5.5 (22%), 5.8 (3.6%), 5.9 (8.0%) and 5c (38%). Serogroup C expressed class 5 epitopes 5.1 (81%), 5.4 (35%), 5.5 (33%) and 5.9 (5.0%); and serogroup A showed reactivity directed at the class 5 protein 5c (47%); and reactivity was present with the new monoclonal antibody, 5.9 (5.5%). We conclude that the two new MAbs are useful in detecting important group B, class 5 antigens, and that a broad selection of serogroup B, class 5 proteins would be required for an effective vaccine based on the class 5 proteins.

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Section: Discussionmentioning

confidence: 99%

“…As a control, bacteria were incubated in NS-1% BSA. All samples were prepared in triplicate [34]. Positive control MAbs included meningococcal specific serotyping and subtyping.…”

Section: Reactivity Of the New Mabs With The Surface Of N Meningitidmentioning

confidence: 99%

Expression of class 5 antigens by meningococcal strains obtained from patients in Brazil and evaluation of two new monoclonal antibodies

E¹,

Zollinger²

2001

Braz J Infect Dis

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“…The control consisted of bacteria incubated in 1% BSA in saline. All samples were analyzed in triplicate [15]. The positive controls were meningococcal MAbs specific for immunotypes L8 (1C31B8Br4) and L1 (1B81C3 Br5) [20].…”

Section: Determination Of Mab 5f81a4 Reactivity With the Surface Of Nmentioning

confidence: 99%

Production of monoclonal antibody to subtype 9 of Neisseria meningitidis and the distribution of this subtype in Brazil

Belo¹,

Coutinho²,

Ferraz³

et al. 2004

Braz J Infect Dis

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A new monoclonal antibody (5F81A4P1.9), which is specific for subtype 9 antigen of meningococci, was studied. The antibodies were raised against a previously non-typable (NT) serogroup B strain from Brazilian patients and were found to react with the subtype antigen of prototype reference strains for subtype 9 (M982), as well as with those of homologous strains. The subtype 9 epitope was found in 6.8% of serogroup B strains among 602 strains of Neisseria meningitidis case isolates, including representative isolates from Brazilian states. Subtype P1.9 was predominantly related to serogroup B in Brazil among the isolates collected during the N. meningitidis epidemic in 1992. No significant differences were observed in the occurrence of subtype P1.9 among strains isolated from several Brazilian states. Fluorescence-activated cell-sorter analysis showed that 5F81A4 MAb recognized a 46 kDa protein on the surface of a homologous strain of N. meningitidis (B:4:P1.9). These results, in association with a bactericidal activity assay for 5F81A4, and with experimental passive protection in mice, demonstrated the importance of subtype 9 class 1 proteins of N meningitidis in Brazil. Serotyping is essential for the development of vaccination strategies.

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Study of the new cross reactive monoclonal antibody against Neisseria meningitidis using flow cytometry analysis

Cited by 2 publications

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Expression of class 5 antigens by meningococcal strains obtained from patients in Brazil and evaluation of two new monoclonal antibodies

Expression of class 5 antigens by meningococcal strains obtained from patients in Brazil and evaluation of two new monoclonal antibodies

Production of monoclonal antibody to subtype 9 of Neisseria meningitidis and the distribution of this subtype in Brazil

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